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谢芳,吴官保,姚红艳,冯帅华,杨届.补肾活血汤干预衰老大鼠退变椎间盘模型中经典WNT信号通路β-catenin蛋白含量的研究[J].湖南中医药大学学报英文版,2018,38(3):275-279.[Click to copy
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| 补肾活血汤干预衰老大鼠退变椎间盘模型中经典WNT信号通路β-catenin蛋白含量的研究 |
| 谢芳,吴官保,姚红艳,冯帅华,杨届 |
| (湖南省中医药研究院附属医院, 湖南 长沙 410208;湖南中医药大学, 湖南 长沙 410006) |
| 摘要: |
| 目的 观察补肾活血汤对衰老大鼠退变椎间盘组织形态结构及β-catenin蛋白含量的影响。方法 选取三月龄健康SD大鼠80只，雌雄各半，随机分为空白组、假模型组、模型组、中药低剂量组以及中药高剂量组，每组16只，雌雄各半。除空白组和假模型组外，其余各组注射D-半乳糖建立大鼠衰老动物模型，空白对照组、假模型组与模型组每日生理盐水灌胃，中药低剂量组及中药高剂量组补肾活血汤每日灌胃，持续12周。分别于造模后、灌胃4周后、灌胃8周后、灌胃12周后每组随机抽取雌雄大鼠各2只处死取材，光镜下观察椎间盘组织形态学变化并运用蛋白质印迹法（Western Blot）检测β-catenin蛋白表达差异。结果 与模型组比较，经补肾活血汤干预后，中药低剂量组及中药高剂量组大鼠椎间盘组织形态学评分降低（P<0.05），β-catenin蛋白表达水平降低（P<0.05）。结论 补肾活血汤能有效抑制经典Wnt/β-catenin信号通路中关键蛋白分子β-catenin蛋白的表达，从而起到延缓椎间盘退变的作用。 |
| 关键词: 补肾活血汤 腰椎间盘退变 β-catenin蛋白 |
| DOI：10.3969/j.issn.1674-070X.2018.03.011 |
| Received:April 20, 2017 |
| 基金项目:湖南省中医药管理局资助项目（201221）。 |
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| Effects of Bushen Huoxue Decoction on β-catenin Protein Level of WNT Signal Pathway in Lumbar Intervertebral Disc Degeneration Aging Model Rats |
| XIE Fang,WU Guanbao,YAO Hongyan,FENG Shuaihua,YANG Jie |
| (The Affiliated Hospital of Hunan Academy of Chinese Medicine, Changsha, Hunan 410006, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effect of Bushen Huoxue decoction on the structural and histopathological changes and β-catenin protein in lumbar intervertebral disc degeneration aging model rats. Methods Eighty three-month old SD rats (male-female half and half) were randomly divided into blank control group, sham-model group, model group, TCM low-dose group and TCM high-dose group, 16 rats in each group. Except the blank group and sham-model group, the aging model rats in other groups were established by injecting D-galactose. The rats in blank control group,sham-model group and model group were daily given normal saline by gavage. TCM low-dose and high-dose groups were daily treated with Bushen Huoxue decoction for 12 weeks by gavage. Two male rats and two female rats in each group were randomly executed at after modelling, after 4 weeks of gavage, after 8 weeks of gavage and after 12 weeks of gavage, and samples were selected from dead rats. The histomorphological change of model group was observed by light microscope, and the expression of β-catenin protein was detected by Western Blot method. Results Compared with the model group, the histomorphology score in TCM low-dose and high-dose groups treated with Bushen Huoxue decoction decreased (P<0.05), and the expression of β-catenin protein decreased (P<0.05). Conclusion Buyang Huanwu decoction shows inhibition effect on the expression of key β-catenin protein in the classical Wnt/β-catenin signal pathway, and thus could relieve the degeneration of intervertebral disc. |
| Key words: Bushen Huoxue decoction lumbar intervertebral disc degeneration β-catenin protein |
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