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李玲,卢芳国,陈伶利,向琴,高强,魏科,胡珏,宁毅.小檗碱体外抗耐甲氧西林金黄色葡萄球菌活性的研究[J].湖南中医药大学学报英文版,2018,38(3):254-256.[Click to copy
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| 小檗碱体外抗耐甲氧西林金黄色葡萄球菌活性的研究 |
| 李玲,卢芳国,陈伶利,向琴,高强,魏科,胡珏,宁毅 |
| (湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 研究小檗碱体外抗耐甲氧西林金黄色葡萄球菌（MRSA）活性。方法 运用琼脂扩散法、液体稀释法测定并分析小檗碱对MRSA的抑菌环大小、最低抑菌浓度（MIC）和最低杀菌浓度（MBC）；运用96孔酶标板结晶紫法测定并分析小檗碱对MRSA的最小生物膜清除浓度（MBEC）。结果 与DMSO比较，小檗碱与氯霉素（阳性对照药物）可显著增加MRSA的抑菌环半径（P<0.01），小檗碱对MRSA的MIC为0.2 mg/mL，MBC为0.4 mg/mL；小檗碱对MRSA的MBEC为0.2 mg/mL。结论 小檗碱对MRSA有比较明显的抗菌、抑菌作用，其效应机制可能与其抑制细菌生物膜的形成有关。 |
| 关键词: 小檗碱 耐甲氧西林金黄色葡萄球菌 生物膜 |
| DOI：10.3969/j.issn.1674-070X.2018.03.006 |
| Received:December 04, 2017 |
| 基金项目:国家自然科学基金资助项目（81473468，81503445，81774126，81703919）；湖南省教育厅创新平台开放基金项目（17K067）；湖南省自然科学基金（2016JJ2095，2016JJ3098）；湖南省教育厅优秀青年基金（15B169）；湖南省高校科技创新团队《感染性疾病中医药防治研究》资助项目（15）；湖南省省级精品课程《免疫学基础与病原生物学》资助项目（48）；湖南中医药大学重点学科《基础医学》、《病原生物学》资助项目（1）。 |
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| Effect of Berberine on the Activity of Methicillin-Resistant Staphylococcus aureus in Vitro |
| LI Ling,LU Fangguo,CHEN Lingli,XIANG Qin,GAO Qiang,WEI Ke,HU Jue,NING Yi |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To study berberine on the activity of methicillin-resistant Staphylococcus aureus (MRSA) in vitro. Methods The size of antibacterial annulus, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of MRSA intervened by berberine were measured by Agar diffusion test and broth dilution test. The crystal violet staining method measured on 96 well Elisa-plate was applied to analyze minimal biofilm eradication concentration (MBEC). Results Compare with DMSO, berberine and chloramphenicol (positive control drug) could significantly increase the antibacterial annulus radius of MRSA (P<0.01). The MIC, MBC and MBEC of berberine against MRSA were 0.2 mg/mL, 0.4 mg/mL and 0.2 mg/mL, respectively. Conclusion Berberine shows obvious inhibition effect on MRSA, and its mechanism may be related to the inhibition of bacterial biofilm formation. |
| Key words: berberine methicillin-resistant Staphylococcus aureus biofilm |
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