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秦汉,喻京生,贺莉,王颢筱,高健,姚琬华.祛风活血丸对实验性自身免疫性葡萄膜炎大鼠JAK2 mRNA和STAT3 mRNA表达的影响[J].湖南中医药大学学报英文版,2018,38(3):245-249.[Click to copy
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| 祛风活血丸对实验性自身免疫性葡萄膜炎大鼠JAK2 mRNA和STAT3 mRNA表达的影响 |
| 秦汉,喻京生,贺莉,王颢筱,高健,姚琬华 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410007) |
| 摘要: |
| 目的 观察祛风活血丸对实验性自身免疫性葡萄膜炎大鼠视网膜JAK2及STAT3 mRNA表达的影响。方法 将80只（160眼）雄性Lewis大鼠根据随机数字表法随机抽取16只作为空白组（A），对其余64只大鼠进行实验性自身免疫性葡萄膜炎模型制作。造模成功后，根据随机数字表法将其随机分为模型组（B）、祛风活血丸常规剂量组（C）、祛风活血丸两倍剂量组（D）、熊胆开明片组（E）。干预14 d后用qPCR法检测大鼠视网膜组织中JAK2 mRNA和STAT3 mRNA的相对表达量。结果 JAK2 mRNA：与B组比较，C组、E组表达降低，差异具有统计学意义（P<0.05），D组表达显著降低，差异具有统计学意义（P<0.01）；STAT3 mRNA：与B组比较，C、D、E组表达均降低，差异具有统计学意义（P<0.05）。结论 祛风活血丸能降低实验性自身免疫性葡萄膜炎大鼠JAK2、STAT3 mRNA的表达，抑制JAK2/STAT3信号通路，达到抗炎作用。 |
| 关键词: 实验性自身免疫性葡萄膜炎 祛风活血丸 JAK2/STAT3信号通路 JAK2 mRNA STAT3 mRNA |
| DOI：10.3969/j.issn.1674-070X.2018.03.004 |
| Received:December 17, 2017 |
| 基金项目:国家自然科学基金项目（81273808）；湖南省中医药科研计划项目（201718）；湖南省教育厅科学研究项目（17A164）；湖南省研究生科研创新项目（CX2017B435）；湖南中医药大学研究生创新课题项目（2017CX11）。 |
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| Effects of Qufeng Huoxue Pill on JAK2 mRNA and STAT3 mRNA Expression in Experimental Autoimmune Uveitis Rats |
| QIN Han,YU Jingsheng,HE Li,WANG Haoxiao,GAO Jian,YAO Wanhua |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To observe the effect of Qufeng Huoxue pill on expression of JAK2 mRNA and STAT3 mRNA of retinal ganglion cells in experimental autoimmune uveitis rats. Methods According to the random number table method, 16 rats from 80 male Lewis rats (160 eyes) were randomly selected as the blank group (group A), and the other 64 rats were built for experimental autoimmune uveitis models. After the success of modeling, the rats were randomly divided into model group (group B), conventional dose of Qufeng Huoxue pill group (group C), double doses of Qufeng Huoxue pill group (group D), Xiongdan Kaiming tablet group (group E). After 14 days of administration, the relative expression of JAK2 mRNA and STAT3 mRNA in retinal tissue of rat was detected by Real-time fluorescence quantitative PCR. Results JAK2 mRNA:compared with group B, the expression of group C and group E decreased obviously, the difference was with statistical significance (P<0.05), the expression of group D decreased significantly (P<0.01). STAT3 mRNA:compared with group B, the expression of group C, D and E decreased significantly, there was statistical significance (P<0.05). Conclusion Qufeng Huoxue pill could reduce the expression of JAK2 mRNA and STAT3 mRNA in experimental autoimmune uveitis rats and inhibit the JAK2/STAT3 signaling pathway to achieve anti-inflammatory effect. |
| Key words: experimental autoimmune uveitis Qufeng Huoxue pill JAK2/STAT3 signal pathway JAK2 mRNA STAT3 mRNA |
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