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李迎秋,张国民,朱义刚,刘洋,肖海林,黎啸风,夏珊,周晋,赵爱明,何清湖.血府逐瘀汤对动脉粥样硬化家兔主动脉组织单核细胞趋化因子-1表达的影响[J].湖南中医药大学学报英文版,2017,37(12):1312-1315.[Click to copy
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| 血府逐瘀汤对动脉粥样硬化家兔主动脉组织单核细胞趋化因子-1表达的影响 |
| 李迎秋,张国民,朱义刚,刘洋,肖海林,黎啸风,夏珊,周晋,赵爱明,何清湖 |
| (湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察血府逐瘀汤对动脉粥样硬化家兔主动脉组织单核细胞趋化因子-1（monocyte chemotactic protein-1，MCP-1）表达的影响，并探讨血府逐瘀汤抗动脉粥样硬化的机制。方法 将24只清洁级日本雄性大耳家兔，随机分为3组，每组8只：空白组给予普通饲料；模型组先给予高胆固醇高脂饲料100 g/d后给予普通饲料；血府逐瘀汤组在模型组喂养的基础上，灌胃给予血府逐瘀汤，每天1次。喂养8周后，采血测定家兔血脂全套（TG、TC、LDL）；应用光学显微镜观察兔主动脉切片的病理改变，测定其主动脉粥样硬化斑块灰度及斑块面积；利用免疫组织化学方法测定主动脉MCP-1蛋白的表达。结果 主动脉图像分析血府逐瘀汤组家兔胸主动脉斑块灰度及斑块面积分别低于模型组（P<0.01）；与空白组及血府逐瘀汤组相比，模型组家兔血清TC，TG，LDL均明显升高（P<0.05）。血府逐瘀汤组家兔血清TC，LDL的水平却明显高于空白组（P<0.01）。模型组家兔动脉粥样硬化家兔主动脉MCP-1蛋白表达平均灰度值明显高于血府逐瘀汤组和空白组（P<0.01）。结论 血府逐瘀汤可以降低动脉粥样硬化家兔主动脉的MCP-1蛋白表达水平；血府逐瘀汤抗动脉粥样硬化的机制可能与其降低MCP-1蛋白表达水平有关。 |
| 关键词: 血府逐瘀汤 单核细胞趋化因子-l 动脉粥样硬化 兔 |
| DOI：10.3969/j.issn.1674-070X.2017.12.004 |
| Received:January 10, 2017 |
| 基金项目:国家自然科学基金面上项目（81373641）；2012年度湖南省大学生研究性学习和创新性实验计划项目（2012181）；2013年度湖南省中医药科研计划项目（2013161）；2014年湖南省科学技术厅课题（2014SK3050）。 |
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| Effects of Xuefu Zhuyu Decoction on the Expression of Monocyte Chemoattractant Protein-1 in Aortic Tissue of Atherosclerosis Rabbits |
| LI Yingqiu,ZHANG Guomin,ZHU Yigang,LIU Yang,XIAO Hailin,LI Xiaofeng,XIAN Shan,ZHOU Jin,ZHAO Aiming,HE Qinghu |
| (Hunan University of Chinese Medine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To observe the effect of Xuefu Zhuyu decoction on the expression of monocyte chemoattractant protein-1 (MCP-1) in aortic tissue of atherosclerosis rabbits. Methods Twenty-four male rabbits were randomly divided into three groups, 8 rats in each group. The control group was fed with normal diet, the model group was given with 100g/d high fat and cholesterol diet before feeding normal diet; and Xuefu Zhuyu decoction group was administrated with Xuefu Zhuyu decoction, once daily. The concentrations of serum total cholesterol (TC), total triglyceride(TG), low density lipoprotein (LDL) were measured after eight weeks for feed. The pathology changes of the pathological section, degree and atherosclerotic plaque mean gray value and plaque area were observed by optical microscope. The expression of MCP-1 in atherosclerosis rabbits was detected by immunochemistry. Results The aortic atherosclerotic plaque mean gray value and plaque area of Xuefu Zhuyu decoction group were lower than those of the model group (P<0.01). Compared with the blank group and Xuefu Zhuyu decoction group, the serum TC, TG and LDL of the model group increased obviously (P<0.05). The level of TC and LDL in Xuefu Zhuyu decoction was significantly higher thabn those in blank group (P<0.01). The average gray value of the expression of MCP-1 in the model group was significantly higher than those in the Xuefu Zhuyu decoction group and blank group (P<0.01). Conclusion Xuefu Zhuyu decoction could decrease the expression of MCP-1 in aortic tissue of atherosclerosis rabbits. Its mechanism may be related with the lower MCP-1. |
| Key words: Xuefu Zhuyu decoction monocyte chemoattractant protein-1 atherosclerosis rabbits |
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