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陈梦,赵丕文,孙丽萍,武虹波,赵笛.羟基红花黄色素A对雌激素效应相关蛋白表达的影响[J].湖南中医药大学学报英文版,2017,37(11):1218-1221.[Click to copy
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| 羟基红花黄色素A对雌激素效应相关蛋白表达的影响 |
| 陈梦,赵丕文,孙丽萍,武虹波,赵笛 |
| (北京中医药大学中医学院, 北京 100029) |
| 摘要: |
| 目的 探讨羟基红花黄色素A（hydroxysafflor yellow A，HSYA）对雌激素效应相关蛋白表达的影响。方法 培养雌激素受体阳性细胞T47D和特异性雌激素受体（estrogen receptor，ER）阴性细胞SK-BR-3，将1×10-7mol/L-1×10-9mol/L三种梯度浓度的HSYA作用于两种细胞，通过蛋白质免疫印迹法检测雌激素受体ERα及ERβ、细胞外调解蛋白激酶1/2（ERK1/2）及p-ERK1/2、孕激素受体（progesterone receptor，PR）、抑癌基因P27等蛋白的表达情况。结果 与空白对照组相比，在T47D细胞中，高浓度的HSYA促进了ERα、ERβ、ERK1/2、p-ERK1/2、PR的表达，抑制了P27的表达（P<0.01）；在SK-BR-3细胞中，HSYA显著抑制了ERK1/2的表达，但对p-ERK1/2的表达影响不明显（P>0.05）。结论 HSYA在细胞内影响雌激素效应相关蛋白的表达情况不同，可能是通过调节ER表达及激活ERK信号通路而发挥雌激素样作用。 |
| 关键词: 羟基红花黄色素A Western Blot T47D细胞 SK-BR-3细胞 |
| DOI：10.3969/j.issn.1674-070X.2017.11.012 |
| Received:December 23, 2016 |
| 基金项目:国家自然科学青年基金（81673764）；北京中医药大学自主科研课题（2017-JYB-JS-001）。 |
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| Influence of Hydroxysafflor Yellow A on Related Protein Expression of Estrogenic Effects |
| CHEN Meng,ZHAO Piwen,SUN Liping,WU Hongbo,ZHAO Di |
| (School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China) |
| Abstract: |
| Objective To explore the effect of hydroxylsafflor yellow A (HSYA) on related protein of estrogenic effect. Methods The positive cells T47D and ER, and negative cells SK-BR-3 of estrogen receptor (ER) were cultured, and 1×10-7 mol/L×10-9 mol/L of HSYA act on this two cells., negative cells SK-BR-3. The expression of protein ERα, ERβ, extracellular regulated protein kinases 1/2 (ERK1/2), p-ERK1/2, Progesterone Receptor (PR), P27 was detected by Western Blot. Results Compared with the blank control group, the high concentration HSYA promoted the expression of Erα, ERβ, ERK1/2, p-ERK1/2, PR, while inhibite the expression of P27 in T47D cells (P<0.01). HSYA showed significant inhibitory effects on ERK1/2, while had no obvious effects on p-ERK1/2 in SK-BR-3 cells (P>0.05). Conclusion HSYA has different influnces on related protein of estrogenic effect in the cells, which possiblly through regulating the expression of ER and activating the ERK signaling pathway. |
| Key words: hydroxysafflor yellow A Western Blot T47D cell SK-BR-3 cell |
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