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刘碧源,申可佳,申奏秦旋,付灵梅,熊桀.护卵汤对慢性应激超排卵小鼠卵巢TGF-β1,P-Smad3及FSHR mRNA表达的影响[J].湖南中医药大学学报英文版,2017,37(11):1192-1195.[Click to copy
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| 护卵汤对慢性应激超排卵小鼠卵巢TGF-β1,P-Smad3及FSHR mRNA表达的影响 |
| 刘碧源,申可佳,申奏秦旋,付灵梅,熊桀 |
| (湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察护卵汤对慢性应激超排卵小鼠卵巢转化生长因子-β1（transforming growth factor beta 1，TGF-β1）、信号蛋白P-Smad3及卵泡刺激素受体（follicle-stimulating hormone receptor，FSHR）mRNA表达的影响。方法 建立慢性应激小鼠模型，造模成功后，随机分为模型组、护卵汤组和生长激素组，另设正常组。在超排卵第3天和注射绒毛膜促性腺激素（human chorionic gonadotropin，HCG）24 h后，检测各组卵巢TGF-β1、P-Smad3及FSHR mRNA表达。结果 与模型组比较，在超排卵第3天，护卵汤组和生长激素组TGF-β1、P-Smad3及FSHR mRNA表达均显著升高（P<0.01或P<0.05）；注射HCG 24 h后生长激素组表达升高更明显（P<0.01）。结论 护卵汤可能通过激活TGF-β/Smads信号通路，发挥促进卵泡发育和改善卵巢功能的作用。 |
| 关键词: 护卵汤 慢性应激 超排卵 卵巢转化生长因子-β1 P-Smad3 卵泡刺激素受体 |
| DOI：10.3969/j.issn.1674-070X.2017.11.006 |
| Received:January 17, 2017 |
| 基金项目:国家自然科学基金项目资助（81303003，81403427）。 |
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| Effects of Huluan Decoction on mRNA Expression of TGF-β1, P-Smad3 and FSHR in Super Ovulation Mice with Chronic Stress |
| LIU Biyuan,SHEN Kejia,SHEN Zouqinxuan,FU Lingmei,XIONG Jie |
| (Affiliated Hospital of Hunan Academy of traditional Chinese Medicine, Changsha, Hunan 410006, China;(Hunan University of Chinese Medicine, Changsha, Hunan 410208, China)) |
| Abstract: |
| Objective To investigate the effects of Huluan decoction on mRNA expression of TGF-β1, P-Smad3 and FSHR in super ovulation mice with chronic stress. Methods Chronic stress mice models were established. After modeling, the mice were randomly divided into model group, Huluan decoction group, growth hormone group, and normal group (no movement limit) was also assigned. On the third day of the super ovulation and after 24 hours of injection HCG, ovaries were taken from mice of each group for mRNA analysis of TGF-β1, P-Smad3 and FSHR. Results Compared with the model group, on the third day of the super ovulation, the mRNA expression levels of TGF-1, P-Smad3 and FSHR in growth hormone group and Huluan decoction group were significantly higher than those in the model group (P<0.01 or P<0.05), but growth hormone group increased significantly after 24 hours of the injection HCG (P<0.01). Conclusion Huluan decoction could activate TGF-beta/Smads signaling pathway to promote the follicular development and improve ovarian function in super ovulation mice with chronic stress. |
| Key words: Huluan decoction chronic stress super ovulation TGF-β1 P-Smad3 FSHR |
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