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李迎秋,蒋艳菲,张叔琦,赵雪君,曹卉,杨雪,卓海燕,张国民.壮骨止痛胶囊含药血清对成骨细胞CDK4和p21表达的影响[J].湖南中医药大学学报英文版,2017,37(10):1078-1081.[Click to copy
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| 壮骨止痛胶囊含药血清对成骨细胞CDK4和p21表达的影响 |
| 李迎秋,蒋艳菲,张叔琦,赵雪君,曹卉,杨雪,卓海燕,张国民 |
| (湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察壮骨止痛胶囊含药血清对成骨细胞CDK4和p21表达的影响，探讨壮骨止痛胶囊治疗骨质疏松症的机制。方法 40只SD雌性大鼠随机分为模型组、阳性对照组、壮骨止痛胶囊组、假手术组，每组10只。模型组、阳性对照组以及壮骨止痛胶囊组的SD大鼠制作骨质疏松大鼠模型，假手术组SD大鼠作为对照。不同组SD大鼠喂养不同药物，假手术组和模型组大鼠灌胃生理盐水，阳性对照组灌胃尼尔雌醇，壮骨止痛胶囊组灌胃壮骨止痛胶囊药物，连续喂养13周之后，处死大鼠，提取各组大鼠含药血清备用。另取5只新生SD小鼠颅骨的成骨细胞，应用上述各组含药血清进行细胞培养，连续培养3代后，采用碱性磷酸酶染色和茜素红染色法鉴定成骨细胞，细胞免疫组化法检测各组含药血清培养的成骨细胞CDK4蛋白和p21蛋白的表达。结果 CDK4和p21蛋白阳性表达主要在成骨细胞的细胞核。与假手术组比较，模型组CDK4表达轻度降低，p21表达轻度升高（P>0.05）；与模型组比较，阳性对照组和壮骨止痛胶囊组CDK4表达均升高，p21表达均下降（P<0.01）；与阳性对照组比较，壮骨止痛胶囊组CDK4表达升高，p21表达下降（P<0.01）。结论 壮骨止痛胶囊可通过提高成骨细胞CDK4的表达，抑制p21的表达，从而增加成骨细胞G1期调节蛋白的表达，推动增殖周期的完成，达到治疗骨质疏松症的目的。 |
| 关键词: CDK4 p21 壮骨止痛胶囊 骨质疏松症 |
| DOI：10.3969/j.issn.1674-070X.2017.010.007 |
| Received:October 12, 2016 |
| 基金项目:2014年湖南省教育厅科学研究优秀青年项目（14B130）；2015年度湖南省大学生研究性学习和创新性实验计划项目（2015217，2015220）；2016年度湖南省中医药科研计划重点项目（201612）。 |
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| Effect of Zhuanggu Zhitong Capsule Medicated Serum on the CDK4 and p21 Expression of Osteoblasts |
| LI Yingqiu,JIANG Yanfei,ZHANG Shuqi,ZHAO Xuejun,CAO Hui,YANG Xue,ZHUO Haiyan,ZHANG Guomin |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To obseve the effect of Zhuanggu Zhitong capsule medicated serum on the CDK4 and p21 expression of osteoblasts, and investigate its mechanism of action on osteoporosis. Methods Forty female SD rats were randomly divided into model group, positive control group, Zhuanggu Zhitong capsule group and sham-operation group, 10 rats in each group. The SD osteoblasts model rats excluding rats in sham-operation group were built, and shamp-operation group as the control. Rats in sham-operation and model groups were given normal saline by gavage, rats in the positive control group were administrated with nylestriol, and rats in Zhuanggu Zhitong capsule group were received with Zhuanggu Zhitong capsule. After continuous feeding for 13 weeks, the rats were executed, and then the medicated serum from all rats were prepared. Osteoblasts which obtained from five newborn SD neonatal rats were cultured with the medicated serum to the third generation. The osteoblasts were identified by the alkaline phosphatase dye and the alizarin red dye. The expression of CDK4 protein and p21 protein was detected by immunohistochemistry. Results The positive expressions of CDK4 and p21 were mainly in the nucleus of osteoblasts. In comparison with the sham-operation group, the expression of CDK4 in the model group decreased slightly, and the expression of p21 increased slightly (P>0.05). Compared with the model group, CDK4 expression increased and p21 expression decreased in the positive control group and Zhuanggu Zhitong capsule group (P<0.01). The CDK4 expressions in Zhuanggu Zhitong capsule group were higher than those in the positive control group, while the p21 expression in Zhuanggu Zhitong capsule group significantly lower than those in the positive control group (P<0.01). Conclusion Zhuanggu Zhitong capsule could improve CDK4 expression of osteoblasts, and inhibit the expression of p21, thereby increase the expression of osteoblast G1 phase regulatory protein, promote the proliferation cycle, and then achieve the purpose of treatment of osteoporosis. |
| Key words: CDK4 p21 Zhuanggu Zhitong capsule osteoporosis |
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