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沈慧,刘文,姚小磊,杨志波.竹黄颗粒介导IL-22/miR-330调控角质形成细胞增殖的机制研究[J].湖南中医药大学学报英文版,2017,37(7):708-714.[Click to copy
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| 竹黄颗粒介导IL-22/miR-330调控角质形成细胞增殖的机制研究 |
| 沈慧,刘文,姚小磊,杨志波 |
| (湖南中医药大学第二附属医院, 湖南 长沙 410005;广西中医药大学附属瑞康医院, 广西 南宁 530011) |
| 摘要: |
| 目的 研究竹黄颗粒干预IL-22/miR-330调控角质形成细胞增殖的分子机制。方法 IL-22处理HaCaT和HKCs细胞，应用QPCR技术检测细胞中miR-330的表达变化；之后慢病毒感染HaCaT和HKCs细胞，实现miR-330的过表达和干扰，通过CCK8和BrdU技术检测细胞增殖情况；采用CCK8、Western blot、QPCR、ELISA实验分别检测不同浓度竹黄颗粒处理HaCaT、HKCs后细胞增殖能力，IL-22蛋白表达水平，miR-330表达水平及细胞分泌IL-22含量的变化。结果 IL-22处理后的HaCaT和HKCs细胞中miR-330的表达水平显著下调（P<0.01），而细胞增殖能力显著提升（P<0.01）；过表达miR-330后HaCaT和HKCs细胞增殖能力显著下降（P<0.05），干扰miR-330后HaCaT和HKCs细胞增殖能力显著上升（P<0.05）；竹黄颗粒处理HaCaT、HKCs后，细胞增殖能力下降（P<0.01），IL-22蛋白表达水平显著下降（P<0.01），miR-330表达水平显著上升（P<0.01），细胞分泌IL-22含量显著下降（P<0.01）。结论 竹黄颗粒可以通过抑制IL-22的表达，促进miR-330的表达，从而抑制角质形成细胞增殖。 |
| 关键词: 银屑病 竹黄颗粒 IL-22 miR-330 角质形成细胞 |
| DOI：10.3969/j.issn.1674-070X.2017.07.004 |
| Received:May 11, 2017 |
| 基金项目:国家自然科学基金面上资助项目（81573985）。 |
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| Mechanism of Zhuhuang Granule Intervenes IL-22/miR-330 in Regulating Keratinocyte Proliferation |
| SHEN Hui,LIU Wen,YAO Xiaolei,YANG Zhibo |
| (The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China;Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning, Guangxi 530011, China) |
| Abstract: |
| Objective To study the molecular mechanism of IL-22/miR-330 intervened by Zhuhuang granule in regulating the proliferation of keratinocytes. Methods The expression of miR-330 in HaCaT and HKCs cells after IL-22 intervention was detected by QPCR technique. Overexpression and interference of miR-330 in HaCaT and HKCs cells were achieved by lentivirus infection, their cell proliferation was detected by CCK8 and BrdU. The cell proliferation ability, the expression of IL-22 protein, the expression level of miR-330 and the secretion of IL-22 in HaCaT and HKCs were determined by CCK8, Western blot, QPCR and ELISA, respectively. Results The expression of miR-330 in HaCaT and HKCs cells significantly decreased after treatment with IL-22 (P<0.01), and the cell proliferation ability of HaCaT and HKCs cells was significantly improved (P<0.01). The cell proliferation ability of HaCaT and HKCs cells significantly decreased after miR-330 overexpression (P<0.05). After interference of miR-330 expression, cell proliferation ability of HaCaT and HKCs had a significant increase (P<0.05). Cell proliferation ability of HaCaT and HKCs treated by different concentrations of Zhuhuang granule decreased (P<0.01). The expression of IL-22 protein decreased significantly (P<0.01), the expression level of miR-330 increased significantly (P<0.01), and the content of IL-22 decreased significantly (P<0.01). Conclusion Zhuhuang granule could inhibit the expression of IL-22, promote the expression of miR-330 and inhibit the proliferation of keratinocytes. |
| Key words: psoriasis Zhuhuang granule IL-22 miR-330 keratinocytes |
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