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陈林,崔培梧,鲁耀邦,廖彦,张志丽,张书航,黄绍国,易刚强.茉莉酸甲酯对茯苓三萜生物合成的调控研究[J].湖南中医药大学学报英文版,2017,37(6):606-610.[Click to copy
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| 茉莉酸甲酯对茯苓三萜生物合成的调控研究 |
| 陈林,崔培梧,鲁耀邦,廖彦,张志丽,张书航,黄绍国,易刚强 |
| (湖南中医药大学药学院, 湖南 长沙 412008;湖南中医药大学中药药性与药效实验室, 湖南 长沙 410208) |
| 摘要: |
| 目的 探索茉莉酸甲酯（methyl jasmonate，MeJA）对茯苓三萜生物合成的调控效应及其机制。方法 从MeJA添加量、添加时间角度考察MeJA对茯苓三萜生物合成的调控效应，确定最佳调控策略，在此基础上采用荧光定量PCR（Real time PCR，RT-PCR）技术分析MeJA对茯苓甲羟戊酸途径法尼基焦磷酸合成酶基因（fps）和鲨烯合酶基因（sqs）基因表达水平的影响，揭示其调控机制。结果 MeJA可显著促进茯苓三萜的生物合成，最佳添加策略为发酵4 d添加150 μmol/L的MeJA，此时茯苓三萜得率可达20.95 mg/L，为空白组的1.55倍、吐温-80组的1.32倍；RT-PCR结果显示MeJA可使fps、sqs基因表达水平显著上调，分别为吐温-80对照组的1.17倍、788.70倍。结论 MeJA通过显著上调sqs基因表达实现茯苓三萜生物合成的过程强化，为一种有效的促进茯苓三萜合成的外源调控因子。 |
| 关键词: 茯苓 三萜 生物合成 发酵调控 茉莉酸甲酯 |
| DOI：10.3969/j.issn.1674-070X.2017.06.007 |
| Received:December 29, 2016 |
| 基金项目:湖南省发改委科研计划（湘发改高技〔2014〕1199号）；湖南省中医药科研计划重点项目（201621）；湖南省教育厅科研计划（16C1219）；湖南省中药粉体与创新药物省部共建国家重点实验室培育基地开放基金（ZYFT201404）；湖南中医药大学研究性学习和创新性实验计划（201616）；湖湘中药资源保护与利用协同创新中心开放基金（00175-12）；湖南中医药大学生物工程重点学科共同资助。 |
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| Study on Regulation of Triterpenoids Biosynthesis in Wolfiporia cocos Submerged Fermentation System by Methyl Jasmonate |
| CHEN Lin,CUI Peiwu,LU Yaobang,LIAO Yan,ZHANG Zhili,ZHANG Shuhang,HUANG Shaoguo,YI Gangqiang |
| (College of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;TCM Potency & Efficacy Laboratory, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To explore the regulation effects and its molecular mechanism of methyl jasmonate (MeJA) on triterpenoid biosynthesis in submerged fermentation system of WolfiPoria cocos. Methods The effects of addition amount and feeding time of MeJA on Poria cocos triterpenoid biosynthesis were discussed, respectively. Besides, to illustrate regulation mechanism of MeJA, real time PCR (RT-PCR) was employed to analysis the expression level of fps and sqs in mevalonate (MVA) pathway of WolfiPoria cocos. Results The optimal regulation strategy was adding 150 μmol/L MeJA on 4th day. Under this condition, the maximum content can reach up to 20.95 mg/L, which was 1.55 times of blank group, 1.32 times of Tween-80 group. RT-PCR results showed that fps and sqs gene expression level both increased significantly, which were 1.17 times, 788.70 times of that in Tween-80 group, respectively. Conclusion MeJA, as an effective exogenous regulatory factor for increasing triterpenoid biosynthesis level of WolfiPoria cocos, could efficiently improve triterpenoid biosynthesis level in WolfiPoria cocos submerged fermentation system. |
| Key words: WolfiPoria cocos triterpenoids biosynthesis regulation of fermentation methyl jasmonate |
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