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周兴,周青,何清湖.雄蚕益肾方对迟发性性腺功能低下症大鼠睾丸组织病理变化与超微结构的影响[J].湖南中医药大学学报英文版,2017,37(6):586-590.[Click to copy
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| 雄蚕益肾方对迟发性性腺功能低下症大鼠睾丸组织病理变化与超微结构的影响 |
| 周兴,周青,何清湖 |
| (湖南中医药大学第一附属医院, 湖南 长沙 410007;湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 探讨雄蚕益肾方对迟发性性腺功能低下症（late-onset hypogonadism，LOH）大鼠睾丸组织病理变化和超微结构的影响。方法 采用"退役种鼠（40周龄）+复合情志刺激+孤养"方法，建立LOH大鼠模型，随机分为模型组、睾酮组、雄蚕益肾方组（雄蚕组），另设立正常对照组（8周龄），药物干预4周，末次给药后24 h处死动物，检测血清睾酮水平、睾丸指数，分别以光学显微镜和透射电子显微镜观察睾丸组织的病理变化与超微结构。结果 模型组、睾酮组、雄蚕组之间睾丸指数比较，差异均无统计学意义（P>0.05），LOH大鼠睾丸组织出现曲细精管结构紊乱，精子大量减少，睾丸间质变少等病理变化以及线粒体数量明显减少，大量线粒体水肿等超微结构改变，雄蚕组病理变化和超微结构均得到改善。结论 LOH大鼠可出现睾丸组织器官水平和细胞水平的病理变化及超微结构改变，雄蚕益肾方可能能通过改善其病理变化和睾丸间质细胞超微结构而发挥作用。 |
| 关键词: 雄蚕益肾方 迟发性性腺功能低下症 病理变化 超微结构 |
| DOI：10.3969/j.issn.1674-070X.2017.06.002 |
| Received:August 08, 2016 |
| 基金项目:国家自然科学基金（81202706，81673984）；湖南省教育厅优秀青年项目（16B200）。 |
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| Effects of Xiongcan Yishen Prescription on Pathological Changes and Ultrastructure of Testicular Tissue in the Late-Onset Hypogonadism Rats |
| ZHOU Xing,ZHOU Qing,HE Qinghu |
| (The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To study the effects of Xiongcan Yishen formula on the pathological changes and the ultrastructure of the testicular tissue in the late-onset hypogonadism (LOH) rats. Methods The LOH rat models were established by using retired mated rats (40 weeks) which fed separately and given various emotional stimulation. The rats were randomly divided into model group, testosterone group, Xiongcan group, and establishment of control group (8 weeks), the medicine intervention was for 4 weeks. The rats were sacrificed 24 hours later after last medication. The serum testosterone and testicle mass index was detected, the ultrastructure and pathological changes of the testicular tissue were observed by optical microscope and transmission electron microscope. Results The rat testicular indexes between model group, testosterone group, Xiongcan group were significantly different (P>0.05). The LOH model rats have the following pathological changes such as structure disturbance of seminiferous tubules, spermatogenesis loss, testis interstitial loss, and the ultrastructural changes such as mitochondria decreases and obvious edema of mitochondria. The pathological changes and the ultrastructures in Xiongcan group were improved. Conclusion LOH rats appear the pathological changes and the ultrastructural changes of the testicular tissue at the organic level and the cellular level. Xiongcan Yishen prescription could ameliorating its pathological changes and the ultrastructural changes of leydig cells. |
| Key words: Xiongcan Yishen prescription late-onset hypogonadism pathological changes ultrastructure |
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