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廖俞强,邓奕辉,颜佳博,李钰佳,李定祥.滋阴活血解毒方对AGEs诱导VEC损伤黏附分子及凝血相关因子表达的影响[J].湖南中医药大学学报英文版,2017,37(5):485-488.[Click to copy
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| 滋阴活血解毒方对AGEs诱导VEC损伤黏附分子及凝血相关因子表达的影响 |
| 廖俞强,邓奕辉,颜佳博,李钰佳,李定祥 |
| (湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察滋阴活血解毒方对糖基化终末产物（advanced glycation end-producs，AGEs）诱导血管内皮细胞（vascular endothelial cell，VEC）损伤的影响及其黏附分子、凝血相关因子表达的变化，进一步探讨该方对AGEs诱导VEC损伤的保护作用。方法 以人脐静脉内皮细胞（human umbilical vein endothelial cells，HUVEC）作为实验对象，以终浓度（100 mg/L）的AGEs诱导HUVEC损伤。各模型组及给药组细胞分别继续培养12、24、48 h。在显微镜下观察各组细胞形态变化，并以Western-blot法检测各组黏附分子VCAM-1、ICAM-1的表达；以RT-PCR方法检测TF、TM mRNA表达。结果 相对于空白组细胞，模型组细胞出现异常增殖，细胞数量明显增多，多数细胞变形皱缩，胞膜轮廓模糊，细胞内出现粗糙样颗粒变化，VCAM-1、ICAM-1、TF表达明显上调，TM表达下调，且在作用24 h后差异有显著性意义（P<0.01）；相同时间点，给药组较模型组圆缩形细胞减少，能下调VCAM-1、ICAM-1、TF表达，上调TM表达，且随着作用时间的延长更明显（P<0.01）。结论 滋阴活血解毒方能抑制AGEs对VEC的损伤，降低黏附分子的表达，下调表达组织因子mRNA转录，上调血栓调节素mRNA转录。 |
| 关键词: 滋阴活血解毒方 糖基化终末产物 人脐静脉内皮细胞 VCAM-1 ICAM-1 TF TM |
| DOI：10.3969/j.issn.1674-070X.2017.05.006 |
| Received:December 18, 2016 |
| 基金项目:湖南省自然科学基金项目资助（14JJ2113）；湖南省中医药科研基金资助项目（201606）；湖南省教育厅资助项目（16K063）资助；中西医结合基础湖南省重点学科资助。 |
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| Influence of Ziyin Huoxue Jiedu Decoction on Expression of Adhesion Molecules and Blood Coagulation Related Factors in Vascular Endothelial Cells Induced by Advanced Glycation End-Products |
| LIAO Yuqiang,DENG Yihui,YAN Jiabo,LI Yujia,LI Dingxiang |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To investigate the influence of Ziyin Huoxue Jiedu decoction on the expression of adhesion molecules and blood coagulation related factors in the vascular endothelial cells (VEC) induced by advanced glycation end-products (AGEs). Methods The human umbilical vein endothelial cell as the subjects, the human umbilical vein endothelial cells (HUVEC) damage were induced by AGEs with the final concentration (100 mg/L), and the cells were continually cultured for 12, 24, 48 h. The cell morphological changes were observed, the dhesion molecule VCAM-1 and ICAM-1 expression were determined by western-blot. The TF and TM mRNA expression were detected by RT-PCR method. Results Compared with the blank group, the model group cell was abnormal proliferation, cell number increased obviously, most of the cell deformation shrinkage, cell membrane hazy outline, a rough sample are observed in the cell. The VCAM 1, ICAM-1, TF expression significantly raised, TM expression reduced. There was significant diffrence after 24 h(P<0.01). Compared with the model group, the VCAM-1, ICAM-1, TF expression in medicine group down-regulated, TM expression up-regulated, the effect was stronger along with time(P<0.01). Conclusion Ziyin Huoxue Jiedu decocotion could inhibit the damage of VEC induced by AGEs, reduce the expression of adhesion molecules, down-regulate the expression of tissue factor mRNA transcription, up-regulate thrombomodulin mRNA transcription. |
| Key words: Ziyin Huoxue Jiedu decocotion advanced glycation end-products human umbilical vein endothelial cells VCAM-1 ICAM-1 TF TM |
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