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肖小芹,舒圆月,邓桂明,葛金文,杨立平,陈镇,欧阳林旗,向彪.乌药水提液对腹泻型肠易激综合征模型大鼠Ghrelin、MTL、SP、Sec水平的影响[J].湖南中医药大学学报英文版,2017,37(5):477-480.[Click to copy
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| 乌药水提液对腹泻型肠易激综合征模型大鼠Ghrelin、MTL、SP、Sec水平的影响 |
| 肖小芹,舒圆月,邓桂明,葛金文,杨立平,陈镇,欧阳林旗,向彪 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410007;湖南省肿瘤医院, 湖南 长沙 410013) |
| 摘要: |
| 目的 探讨乌药水提液对腹泻型肠易激综合征（diarrhea-predominant irritable bowel syndrome， IBS-D）大鼠血清生长激素释放肽（Ghrelin）、胃动素（Motilin，MTL）、P物质（P substance，SP）、促胰液素（Secretin，Sec）水平的影响。方法 将60只大鼠随机分成6组，采用单只孤养1周+番泻叶煎剂灌胃2周+慢性束缚应激1周造模法建立IBS-D大鼠模型，分别给予乌药水提液低、中、高剂量，匹维溴铵灌胃干预。采用酶联免疫吸附法（ELISA）检测各组大鼠血清Ghrelin、MTL、SP、Sec含量。结果 与空白对照组比较，模型对照组SP、 Ghrelin、MTL水平显著升高，Sec水平降低（P<0.01），提示造模成功；与模型对照组比较，乌药水提液低、中、高剂量组以及阳性对照组血清SP、MTL水平均明显降低，Sec水平均显著升高（P<0.01），乌药水提液低、中、高剂量组以及阳性对照组对Ghrelin影响无显著差异（P>0.05）。结论 乌药水提液可能通过降低大鼠血清SP、MTL水平来改善IBS-D大鼠腹痛、腹泻症状。 |
| 关键词: 腹泻型肠易激综合征 乌药 生长激素释放肽 胃动素 P物质 促胰液素 |
| DOI：10.3969/j.issn.1674-070X.2017.05.004 |
| Received:November 02, 2016 |
| 基金项目:湖南省教育厅重点项目（14A107）；湖南省中医药科研计划项目（201429、2013135）；湖南省教育厅项目（15C1041）；中国博士后基金项目（2016T90752、2014M550420）。 |
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| Effect of Water Extract of Lindera Aggregata on Ghrelin, MTL, SP and Sec Levels in Rats With Diarrhea-Predominant Irritable Bowel Syndrome |
| XIAO Xiaoqin,SHU Yuanyue,DENG Guiming,GE Jinwen,YANG Liping,CHEN Zhen,OUYANG Linqi,XIANG Biao |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Hunan Cancer Hospital, Changsha, Hunan 410013, China) |
| Abstract: |
| Objective To investigate the water extracts of Lindera aggregata on Ghrelin, MTL, SP and Sec levels in rats with diarrhea-predominant irritable bowel syndrome. Methods 60 rats were randomly divided into 6 groups. Using a single solitary 1 weeks, gavage of sennoside for 2 weeks and chronic restraint 1 weeks to establish the rat model of IBS-D. The model rats were administrated with low, medium, high dose of water extracts of Lindera aggregata, and pinaverium bromide. The levels of SP, MTL, Sec and Ghrelin in serum of rats were measured by using enzyme linked immunosorbent assay (ELISA). Results Compared with the blank control group, SP, Ghrelin and MTL levels significantly increased in the model control group, Sec was lower than the control group (P<0.01). Compared with the model group, the plasma levels of SP, MTL in the low, medium, high dose water extracts groups and pinaverium bromide group decreased, the Sec levels significantly increased, with statistical significance (P<0.01), while Ghrelin in the above four groups had no statistical significance (P>0.05). Conclusion The water extracts of Lindera aggregata could improve the abdominal pain and diarrhea of IBS-D model rats by reducing the plasma levels of SP and MTL. |
| Key words: diarrhea-predominant irritable bowel syndrome Lindera aggregata growth hormone releasing peptide motilin substance-p secretin |
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