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彭俊,欧阳云,谭涵宇,李文娟,吴权龙,彭清华.密蒙花滴眼液对去势雄兔干眼症泪腺细胞炎症因子TNF-α、IL-1β的影响[J].湖南中医药大学学报英文版,2017,37(5):469-472.[Click to copy
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| 密蒙花滴眼液对去势雄兔干眼症泪腺细胞炎症因子TNF-α、IL-1β的影响 |
| 彭俊,欧阳云,谭涵宇,李文娟,吴权龙,彭清华 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学, 湖南 长沙 410208;盐城市中医院, 江苏 盐城 224001) |
| 摘要: |
| 目的 观察不同浓度密蒙花滴眼液对去势雄兔角结膜干燥症泪腺细胞炎症因子TNF-α、IL-1β的影响，并探讨其疗效。方法 将42只雄兔随机分为：空白组（A），模型组（B），密蒙花滴眼液低（C）、中（D）、高（E）浓度组，安慰剂组（F），睾酮组（G），每组6只。除A组外各组切除双侧睾丸及附睾。术后第3天开始C-F组给予滴眼液，3次/d；G组注射丙酸睾酮，每3天1次。各组术前和术后第4周各测量一次SIT和BUT。4周后处死兔子，摘取双眼泪腺，采用免疫组化法检测泪腺中TNF-α和IL-1β，并进行统计学分析。结果 （1）SIT与BUT：B、F组前后比较，B组与其他各组及C组与D、E、G组比较，差异均有统计学意义（P<0.01）。（2）治疗后各组TNF-α、IL-1β比较：B、F组与其他各组比较，差异有统计学意义（P<0.01）；C组与D、E、G组比较，差异均有统计学意义（P<0.05）。（3）TNF-α、IL-1β表达结果：A、G组未见表达；B、F组大量表达于细胞膜和细胞浆中，呈棕黄色颗粒；C、D、E组散见表达。结论 密蒙花滴眼液具有与雄激素相似的抑制炎症因子TNF-α和IL-1β表达的作用，但弱于雄激素。密蒙花滴眼液中、高浓度作用无明显差异，但均较低浓度要优。 |
| 关键词: 干眼症 密蒙花滴眼液 去势雄兔 泪腺细胞 炎症因子 |
| DOI：10.3969/j.issn.1674-070X.2017.05.002 |
| Received:March 23, 2016 |
| 基金项目:国家自然科学基金面上资助项目（30772824，81574031）；湖南省高层次卫生人才“225”工程培养项目资助；湖南省科技厅科研基金资助项目（2015SF2016-6）；湖南省发展改革委科研基金资助项目（湘发改投资[2014]658号）；长沙市科技计划重大专项（K1501014-31）；国家中医药管理局中医眼科学重点学科建设项目。 |
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| Effects of Butterflybush Flower Eye Drops on Inflammatory Factors TNF-α and IL-1β in Lacrimal Gland Cells of Castrated Male Rabbits with Xerophthalmia |
| PENG Jun,OUYANG Yun,TAN Hanyu,LI Wenjuan,WU Quanlong,PENG Qinghua |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Yancheng Traditional Chinese Medicine Hospital, Yancheng, Jiangsu 224001, China) |
| Abstract: |
| Objective To observe the effects of butterflybush flower eye drops at different concentrations on inflammatory factors TNF-α and IL-1β in lacrimal gland cells of castrated male rabbits with xerophthalmia. Methods The 42 male rabbits were randomly divided into blank group (A), model group (B), low concentrations butterflybush flower eye drops group (C), medium concentrations butterflybush flower eye drops (D), high concentrations butterflybush flower eye drops (E), placebo group (F) and testosterone group (G), six rabbits in each group. All rabbits of group B to G were cut off bilateral testis and epididymis. The rabbits of group C to F were dropped relevant Eye Drops there times a day. The rabbits of group G were injected testosterone propionate injection every 3 days. The SIT and BUT were determined at 4 weeks before and after operation, respectively. After 4 weeks, the lacrimal gland of all rabbits were killed and clipped. The TNF-α and IL-1β in lacrimal gland cells were tested by immunohistochemical staining method. Results (1) The SIT and BUT were significantly different between group B and F before and after operation (P<0.01). Compared with other groups, SIT and BUT was significantly different in group B before and after operation (P<0.01). Compared with group D, E, G, there was significant difference in group C before and after operation (P<0.01). (2) Comparison of TNF-α and IL-1β after treatment:there was significant difference between group B and F with other groups (P<0.01). Compared with group D, E, G, the indicators in group C was significantly different (P<0.05). (3) The expression of TNF-α and IL-1β were not found in group A and G. In group B and F:TNF-α and IL-1β were expressed in cell membrane and cytoplasm heavyly, showing brownish granules with yellow. In group C, D and E, TNF-α and IL-1β were poorly expressed. Conclusion Butterflybush flower eye drops show a similar effect with androgen on depressing the expression of inflammatory factors TNF-α and IL-1β, but its effect is slightly weaker than the androgen. The depressing effect of medium and high concentration buddleia eye drops is better than the low concentration, while there was no significant difference between medium and high concentration. |
| Key words: xerophthalmia butterflybush flower eye drops castrated male rabbit lacrimal gland cell inflammatory factors |
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