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谢燕燕,卫梅,谢德金,陈剑成,杨德明,荣俊冬,陈礼光,郑郁善.凹叶厚朴用于细胞悬浮培养的愈伤组织的制备研究[J].湖南中医药大学学报英文版,2017,37(4):365-368.[Click to copy
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| 凹叶厚朴用于细胞悬浮培养的愈伤组织的制备研究 |
| 谢燕燕,卫梅,谢德金,陈剑成,杨德明,荣俊冬,陈礼光,郑郁善 |
| (福建农林大学林学院, 福建 福州 350002;福建农林大学园林学院, 福建 福州 350002) |
| 摘要: |
| 目的 研究凹叶厚朴适用于细胞悬浮培养的愈伤组织。方法 通过对基本培养基（B5、MS和1/2MS）、外植体（茎段、顶芽和叶片）、激素浓度及组合进行筛选的方法进行厚朴愈伤组织诱导和继代研究。结果 B5 为最适合厚朴愈伤组织诱导的基本培养基；顶芽是最佳诱导愈伤组织的材料；最适合厚朴愈伤组织诱导的培养基为B5+2.0 mg/L 2，4-D+1.0 mg/L 6-BA；最佳继代培养基为B5+2.0 mg/L NAA+1.0 mg/L 6-BA，愈伤组织生长倍数达到2.87倍。结论 初步筛选出凹叶厚朴用于细胞悬浮培养的愈伤组织，为凹叶厚朴细胞培养奠定基础。 |
| 关键词: 凹叶厚朴 愈伤组织 悬浮培养 |
| DOI：10.3969/j.issn.1674-070X.2017.04.005 |
| Received:September 10, 2016 |
| 基金项目:“十二五”国家科技支撑计划项目（2011BAI01B06）资助；福建省科技创新平台（2008Y2001）资助 |
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| Study on the Preparation of Callus of Magnolia officinalis Using on Cell Suspension Culture |
| XIE Yanyan,WEI Mei,XIE Dejin,CHEN Jiancheng,YANG Deming,RONG Jundong,CHEN Liguang,ZHENG Yushan |
| (College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China) |
| Abstract: |
| Objective To study the callus of Magnolia officinalis suitable for cell suspension culture. Methods Callus induction and subculture study were carried out through the selection of basic mediums (B5, MS and 1/2MS), explants (stems, phyllophores and leaves) and concentrations of hormone. Results The best basic medium for callus induction of Magnolia biloba was B5. Phyllophor is the best explant for callus induction of magnolia biloba. The best medium for callus induction of magnolia biloba was B5+2.0 mg/L 2,4-D+1.0 mg/L 6-BA. The best subculture medium for callus of Magnolia officinalis was B5+2.0 mg/L NAA+1.0 mg/L 6-BA, callus growth rate reached 2.87 times. Conclusion The callus of Magnolia officinalis suitable for cell suspension culture has been preliminary screened, which laying the foundation for cell suspension culture of Magnolia officinalis. |
| Key words: Magnolia officinalis callus suspension culture |
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