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罗佩,侯丽莹,邓丽玲,赵娜,全春梅,杨正望,余曦明.补肾化瘀方对PCOS大鼠子宫内膜整合素αvβ3及LIF表达的影响[J].湖南中医药大学学报英文版,2017,37(3):254-258.[Click to copy
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| 补肾化瘀方对PCOS大鼠子宫内膜整合素αvβ3及LIF表达的影响 |
| 罗佩,侯丽莹,邓丽玲,赵娜,全春梅,杨正望,余曦明 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第一附属医院妇产科, 湖南 长沙 410007) |
| 摘要: |
| 目的 观察补肾化瘀方对多囊卵巢综合征（PCOS）大鼠子宫内膜整合素αvβ3及白血病抑制因子（LIF）的影响。方法 100只SD大鼠随机分为25只为空白对照组，75只为模型制备组，予颈背部皮下注射脱氢表雄酮（dehydroepiandrostexone，DHEA）溶液建立PCOS大鼠模型，判定造模成功后随机分为模型对照组、达英-35组、补肾化瘀方组，分别用蒸馏水、达英-35及补肾化瘀方对大鼠灌胃给药，连续灌胃21 d，停药7 d为1周期，3个周期后停药，按雌雄鼠1：1合笼，并于妊娠第4天处死大鼠，采用免疫组化法检测PCOS大鼠子宫内膜整合素αvβ3及LIF的表达。结果 （1）与空白对照组比较，模型对照组、达英-35组、补肾化瘀方组整合素αvβ3表达均较低（P<0.05）；与模型对照组比较，补肾化瘀方组整合素αvβ3较高（P<0.05），达英-35组整合素αvβ3较低（P>0.05）。（2）与空白对照组比较，模型对照组和达英-35组LIF较低（P<0.05）；与模型对照组比较，补肾化瘀方组LIF较高（P<0.05）。结论 补肾化瘀方可提高PCOS大鼠子宫内膜整合素αvβ3及LIF的表达，这可能是其改善子宫内膜容受性的机制之一。 |
| 关键词: 多囊卵巢综合征 子宫内膜容受性 整合素 白血病抑制因子 补肾化瘀 |
| DOI：10.3969/j.issn.1674-070X.2017.03.006 |
| Received:July 05, 2016 |
| 基金项目:湖南省科技厅计划一般项目（2014TT2007）。 |
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| Effects of Bushen Huayu Precription on the Expression of Endometrial Integrin αvβ3 and LIF in PCOS Rats |
| LUO Pei,HOU Liying,DENG Liling,ZHAO Na,QUAN Chunmei,YANG Zhengwang,YU Ximing |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Department of Gynecology and Obestrics, the First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To explore the influence of Bushen Huayu prescription on endometrial integrin-αvβ3 and leukaemia inhibitory factor(LIF) in polycystic ovary syndrome(PCOS) rats. Methods One hundred SD rats were randomly devided into two groups of 25 rats in control group and 75 rats in model group. PCOS rat modules were to built by injecting dehydroepiandrostexone(DHEA) solution in the back of the neck subcutaneous of rats. After modules built successfully, the selected rats were randomly divided into model group, diane-35 group and Bushen Huayu prescription treatment group, given by gavage with distilled water, diane-35 and Bushen Huayu prescription separately for 21 d. Then stopped 7 d for a cycle. Drug withdraw after 3 discontinuous cycles, female rats were cohabited with male rats according to 1:1. The rats were executed after pregnancy for 4 d. The expressions of endometrial integrin-αvβ3 and LIF were detecting using immunohistomchemical method in PCOS rats. Results (1) Compared with the control group, the expressions of the integrin-αvβ3 were lower in model group, diane-35 group and Bushen huayu prescription group (P<0.05). Compared with the module group, the expressions of the integrin-αvβ3 were higher in Bushen Huayu prescription group (P<0.05) and were lower in diane-35 group (P>0.05). (2) Compared with the control group, the expressions of the LIF were lower in model group, diane-35 group (P<0.05). Compared with the module group, the expressions of the LIF were higher in Bushen Huayu prescription group (P<0.05). Conclusion Bushen Huayu prescription can increase the expressions of endometrium integrin-αvβ3 and LIF in PCOS rats, that may be one of the mechanisms of improving the receptivity of endometrium. |
| Key words: polycystic ovary syndrome endometrial receptivity integrin leukemia inhibitory factor tonifying kidney and removing blood stasis Bushen Huayu prescription |
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