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刘晓丹,成绍武,范婧莹,宋祯彦,李平,邓常清.人参皂苷Rg1对H2O2诱导的HT22细胞凋亡及胞内Ca2+变化的影响[J].湖南中医药大学学报英文版,2017,37(3):236-239.[Click to copy
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| 人参皂苷Rg1对H2O2诱导的HT22细胞凋亡及胞内Ca2+变化的影响 |
| 刘晓丹,成绍武,范婧莹,宋祯彦,李平,邓常清 |
| (湖南中医药大学 中西医结合心脑疾病防治湖南省重点实验室, 湖南 长沙 410218;湖南中医药大学 中西医结合学院, 湖南 长沙 410218;湖南中医药大学 医学院, 湖南 长沙 410218) |
| 摘要: |
| 目的 研究人参皂苷Rg1对H2O2诱导的HT22细胞凋亡及胞内Ca2+浓度变化的影响。方法 以0、6.25、12.5、25、50、100μg/L人参皂苷Rg1预处理细胞24 h，采用Ca2+荧光染料探针Fluo-3/AM负载细胞1 h后，50 mmol/L H2O2刺激细胞，多功能酶标仪测定荧光强度，激光共聚焦显微镜实时监测[Ca2+]i变化，Hoechst 33258染色检测细胞凋亡。结果 H2O2可诱导细胞内Ca2+浓度增加（P<0.01），并增加细胞凋亡率（P<0.01）。不同浓度人参皂苷Rg1可呈剂量依赖性的抑制H2O2诱导的HT22[Ca2+]i增加（P<0.01），抑制细胞凋亡（P<0.01），以50μg/L的作用为最强（P<0.01）。结论 人参皂苷Rg1可通过降低H2O2诱导的HT22细胞内Ca2+水平的升高，抑制氧化应激引起的细胞凋亡。 |
| 关键词: 人参皂苷Rg1 HT22细胞 细胞凋亡 Ca2+浓度 |
| DOI：10.3969/j.issn.1674-070X.2017.03.002 |
| Received:August 30, 2016 |
| 基金项目:国家自然科学基金项目（81473581），湖南省教育厅项目（14C0859），中西医结合防治心脑疾病的相关基础研究湖南省高校科技创新团队项目（2015CXTD），中西医结合基础湖南省重点学科开放基金项目。 |
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| Effect of Ginsenoside Rg1 on H2O2 Induced Apoptosis and Intracellular Ca2+ Concentration in HT22 Cells |
| LIU Xiaodan,CHENG Shaowu,FAN Jingying,SONG Zhenyan,LI Ping,DENG Changqing |
| (Key Laboratory of Hunan Province of Rebrovascular Disease Prevention and Treatment of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;College of Integrated of Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Medical School, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To investigate the effect of ginsenoside Rg1 on H2O2 induced apoptosis and intracellular Ca2+ concentration in HT22 cells. Methods Ginsenoside Rg1 with 0, 6.25, 12.5, 25, 50 and 100μg/L were used to pre-processing HT22 cells for 24 h, separately. Ca2+ fluorescent probe Fluo-3/AM was used to load cells for 1h. Then using 50 mmol/L H2O2 stimulate cells. The fluorescence intensity was measured for microplate reader,[Ca2+]i changes were monitored by scanning confocal microscopy. Apoptosis were tested by Hoechst 33258 staining. Results The Ca2+ concentration (P<0.01) and apoptosis (P<0.01) induced by H2O2 increased significantly. Different concentrations of ginsenoside Rg1 showed dose dependent inhibition in increasing the HT22[Ca2+]i (P<0.01) and apoptosis (P<0.01) induced by H2O2, and the concentation of 50μg/L shows strongest effect (P<0.01). Conclusion The ginsenoside Rg1 could inhibit apoptosis of oxidative apoptosis by reducing the cellular Ca2+ level induced by H2O2. |
| Key words: ginsenoside Rg1 HT22 cell apoptosis Ca2+ concentration |
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