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Quote : 刘余,肖新云,唐标,巩一丁,尹抗抗.长期食用黄豆对小鼠肠道微生物的影响研究[J].湖南中医药大学学报英文版,2017,37(1):26-28.[Click to copy ]
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长期食用黄豆对小鼠肠道微生物的影响研究
刘余,肖新云,唐标,巩一丁,尹抗抗
(湖南中医药大学, 湖南 长沙 410208)
摘要:
    目的 探究长期食用黄豆对小鼠肠道微生物的影响,为日常膳食营养提供依据。方法 先将小鼠按照年龄分为1月龄(幼年)、6月龄(中年)、9月龄(老年)组,每组再按照随机数字表分为对照组、1:1组、1:2组和1:4组,对照组给予标准饲料喂养,1:1组、1:2组和1:4组分别将基础饲料与黄豆按1:1、1:2和1:4关系配比进行饲养。喂养4个月后,采集小鼠盲肠段内容物进行微生物分析。结果 1月龄小鼠食用不同比例的黄豆后,与对照组比较,肠道细菌总数、乳酸菌数、双歧杆菌数和大肠杆菌数均下降明显(P<0.01);6月龄小鼠1:2组的肠道细菌总数、乳酸菌数、双岐杆菌数和大肠杆菌数远远高于其余各组(P<0.01);9月龄小鼠食用不同比例的黄豆后,肠道细菌总数下降,且食用较高比例的黄豆,肠道乳酸菌数、双岐杆菌数和大肠杆菌数与对照组比较,下降明显(P<0.01或P<0.05)。结论 不同年龄段食用不同比例黄豆对肠道微生物的影响不同,6月龄食用1:2黄豆最佳。
关键词:  黄豆  肠道微生物  乳酸菌  双歧杆菌  大肠杆菌  细菌总数
DOI:10.3969/j.issn.1674-070X.2017.01.006
Received:June 02, 2016  
基金项目:湖南省教育厅项目基金(13C678),2013年湖南省大学生研究性学习和创新性实验计划项目基金。
Effect of Long-Term Consumption of Soybean on the Intestinal Microbiota of Mice
LIU Yu,XIAO Xinyun,TANG Biao,GONG Yiding,YIN Kangkang
(Hunan University of Chinese Medicine, Changsha, Hunan 410208, China)
Abstract:
    Objective To explore the impact of long-term consumption of soybean on the intestinal microbial, and to provide a basis for the daily diet. Methods The mice were divided into three groups of 1 month old (infancy), 6 months old (middle aged) and 9 months old (old age) according to the age, and each group was randomly divided into control group, 1:1 group, 1:2 group and 1:4 group. The control group was fed a standard diet, 1:1, 1:2 and 1:4 group were fed by 1:1, 1:2 and 1:4 ratio of basic feed and soybean, respectively. After feeding for 4 months, the content of the microorganism in cecum was collected and analyzed. Results After feeding different proportion of soybean in 1 month old mice, the total number of bacteria, Lactobacillus, Bifidobacterium and E. coli decreased significantly (P<0.01); In 6 months old mice, the number of intestinal bacteria, Lactobacillus, Bifidobacterium and E. coli of 1:2 group was far higher than that of the other groups; After eating different proportions of soybeans of 9 months old mice, the total number of intestinal bacteria decreased, and with the higher proportion of soybean, the number of Lactobacillus, Bifidobacterium and E. coli decreased significantly (P<0.01, P<0.05). Conclusion The effects of different proportions of soybean on the intestinal microflora was different in different age groups, the proportion of 1:2 soybean is the best in 6 months old mice.
Key words:  soybean  intestinal microflora  Lactobacillus  Bifidobacterium  Escherichia coli  total number of bacteria
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