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单增天,田岳凤,李雷勇,吴爱花,王军.不同灸法对免疫抑制兔脾脏指数及细胞因子的影响[J].湖南中医药大学学报英文版,2016,36(11):83-86.[Click to copy
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| 不同灸法对免疫抑制兔脾脏指数及细胞因子的影响 |
| 单增天,田岳凤,李雷勇,吴爱花,王军 |
| (山西中医学院针灸推拿学院, 山西 太原 030619;山西医科大学第二医院, 山西 太原 030001) |
| 摘要: |
| 目的 观察不同灸法对环磷酰胺所致免疫抑制兔脾脏指数及细胞因子IFN-γ、IL-2及IL-4的影响。方法 将大耳白兔50只随机分为空白组、模型组、隔药饼灸组、艾灸组和假药饼灸组5组。除空白组外，其余4组均腹腔注射环磷酰胺60 mg/kg，造成免疫抑制模型。3个治疗组分别给予隔药饼灸、艾灸、假药饼灸治疗，隔日灸，共灸10次。治疗结束次日动物麻醉，取动物脾脏计算其脾脏指数，采用ELISA法测定匀浆上清液中IFN-γ、IL-2、IL-4含量。结果 环磷酰胺造模后，脾脏指数与空白组比较显著升高，差异具有显著的统计学意义（P<0.01），3个治疗组脾脏指数与模型组比较差异均有显著统计学意义（P<0.01），与空白组比较差异无统计学意义（P>0.05）。与空白组比较，艾灸组IFN-γ、IL-4明显降低，模型组、假药饼组IL-4明显降低，差异具有统计学意义（P<0.05）。与模型组比较，艾灸组IFN-γ明显降低，隔药饼灸组IL-4显著升高，差异具有统计学意义（P<0.05）。结论 不同灸法对免疫抑制兔脾脏指数均有调节作用；隔药饼灸可使因环磷酰胺所致下降的IL-4显著提升。 |
| 关键词: 环磷酰胺 隔药饼灸 艾灸 脾脏指数 细胞因子 |
| DOI：10.3969/j.issn.1674-070X.2016.11.022 |
| Received:June 17, 2016 |
| 基金项目:国家自然科学基金面上项目（81373742）。 |
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| Influence of Different Moxibustion on Spleen Index and Cytokines in Immunosuppressive Model Rabbits |
| SHAN Zengtian,TIAN Yuefeng,LI Leiyong,WU Aihua,WANG Jun |
| (Acupuncture and Massage College of Shanxi University of Traditional Chinese Medicine, Shanxi, Taiyuan 0306191, China;The Second Hospital of Shanxi Medical University, Shanxi, Taiyuan 030001, China) |
| Abstract: |
| Objective To observe the inhibitory effect of different moxibustion methods on rabbit spleen index, the contents of IFN-γ, IL-2 and IL-4 in cyclophosphamide induced immunosuppression model rabbits. Methods The 50 rabbits were randomly divided into five groups, namely black group, model group, herb-cake-separated moxibustion group, moxibustion group, sham-cake-separated moxibustion group. In addition to the black group, the other four groups were injected with cyclophosphamide 60 mg/kg to make immunosuppressive model. Three treatment groups were respectively given herb-cake-separated moxibustion, moxibustion, sham-cake-separated moxibustion treatment every other day, for 10 times. The next day after finished moxibustion, the animals were anesthetized, the spleen index was detected, the IFN-γ, IL-2, IL-4 in supernatant of spleen homogenate were determined by ELISA. Results Compared with the black group, the spleen index of model group was significantly higher, it was statistically significant (P<0.01). The spleen index in the three treatment group was statistically significant comparing with the model group (P<0.01), while the diffrence between the treatment groups and blank group was not statistically significant (P>0.05). Compared with the blank group, the IFN-γ and IL-4 in the moxibustion group were lower significantly, the IL-4 in model group and sham-cake-separated group was reduced, the differences were statistically significant (P<0.05). Compared with the model group, the content of IFN-γ was decreased in moxibustion group, and the content of IL-4 increased in herb-cake-separated moxibustion group was increased, the differences were statistically significant (P<0.05). Conclusion Different moxibustion shows a regulating effect on spleen index in immunosuppressive rabbits. The herb-cake-separated moxibustion could significantly increase the decline content of IL-4 induced by cyclophosphamide. |
| Key words: cyclophosphamide herb-cake-separated moxibustion moxibustion spleen index cytokines |
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