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赵国荣,张衎,何宜荣,艾碧琛,肖碧跃,贺又舜,左亚杰,熊焰,陈兰玲,尹周安.清热解毒化浊片对ETM兔肝组织CD14及TNF-α、IL-1表达的影响[J].湖南中医药大学学报英文版,2016,36(9):26-28.[Click to copy
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| 清热解毒化浊片对ETM兔肝组织CD14及TNF-α、IL-1表达的影响 |
| 赵国荣,张衎,何宜荣,艾碧琛,肖碧跃,贺又舜,左亚杰,熊焰,陈兰玲,尹周安 |
| (湖南中医药大学中医学院, 湖南 长沙 410208;广西中医药大学第一附属医院, 广西 南宁 530023;湖南中医药大学第一附属医院, 湖南 长沙 410007) |
| 摘要: |
| 目的 探讨清热解毒化浊片对内毒素血症（Endotoxemia，ETM）模型兔肝功能、肝组织CD14和血清肿瘤坏死因子-α（TNF-α）、白细胞介素-1（IL-1）的影响。方法 将21只新西兰大耳白兔随机分为正常组、模型组、清热解毒化浊片组（清化组），每组7只。将模型组及清化组兔沿耳缘静脉注射大肠杆菌内毒素（LPS，10 μg/kg），于造模后2 h干预，灌胃后2 h心脏采血检测家兔的肝功能、TNF-α和IL-α的变化，采肝脏组织检测CD14及观察肝脏病理形态。结果（1）与模型组比较，清化组ALT、AST上升（P<0.05），肝组织CD14表达及血清TNF-α、IL-1下降（P<0.05）；（2）模型组肝细胞排列结构紊乱，有炎性细胞浸润，且出现空泡样改变，清化组及正常组改变不明显。结论 清热解毒化浊片能下调ETM模型兔CDl4、TNF-α和IL-1的表达，具有多靶点、多效应的干预作用，推测其有阻断早期LPS诱导信号通路的作用。 |
| 关键词: 内毒素血症 清热解毒化浊片 CD14 肿瘤坏死因子-α 白细胞介素-1 茵陈 黄芩 滑石 |
| DOI：10.3969/j.issn.1674-070X.2016.09.006 |
| Received:December 06, 2015 |
| 基金项目:湖南省教育厅重点项目资助（12A101）；湖南中医药大学中医临床基础重点学科资助;湖南中医药大学校级青年基金（校行科字[2013]4号）资助 |
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| Effect of Qingre Jiedu Huazhuo Tablets on Expression of CD14 in Liver Tissue, TNF-α and IL-1 of ETM Rabbits |
| ZHAO Guorong,ZHANG Kan,HE Yirong,AI bichen,XIAO Biyue,HE Youshun,ZUO Yajie,XIONG Yan,Chen Lanling,YIN Zhouan |
| (College of TCM, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, Guangxi 530023, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To investigate the effect of Qingre Jiedu Huazhuo (QJH) tablets on the expression of CD14,TNF-α and IL-1 in liver tissue of ETM rabbits.Methods The 21 New Zealand big-eared rabbits were randomly divided into normal group,model group,QJH tablets group,7 in each group.The ear edge of model group and QJH group was given intravenous injection of 10 μg/kg endotoxin.After 2 hours of modeling,the rabbites were given intervention.Then,the liver function,TNF-α and IL-1 were tested.The CD14 in liver tissue was determined and the pathological morphology was observed.Results (1) Compared with the model group,the ALT and AST of QJH tablets group were increased (P<0.05).The CD14 expression in liver tissue and serum TNF-α and IL-1 were decreased (P<0.05).(2) The liver cell array structure of model group was disorder with inflammatory cells infiltration and a cavity change,but the changes in QJH tablets group and blank group were not obvious.Conclusion Qingre Jiedu Huazhuo tablets with multi-point target and munti-effects can decrease CDl4,TNF-α and IL-1 in ETN rabbits,suggesting the tablets have an effect on the signaling pathway induced by LPS in the early days. |
| Key words: endotoxemia Qingre Jiedu Huazhuo tablets CD14 TNF-a IL-1 oriental wormwood radix scutellariae talc |
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