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刘平安,戴瑜娉,孟小莎,柳诗雨,高凡,张国民.基于TGF-β-Smad通路探讨壮骨止痛方对绝经后骨质疏松症的治疗[J].湖南中医药大学学报英文版,2016,36(7):31-34.[Click to copy
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| 基于TGF-β-Smad通路探讨壮骨止痛方对绝经后骨质疏松症的治疗 |
| 刘平安,戴瑜娉,孟小莎,柳诗雨,高凡,张国民 |
| (湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 探究壮骨止痛方能否通过TGF-β-Smad通路治疗绝经后骨质疏松症，并探索其作用机制。方法 60只雌性大鼠随机分为空白对照组、假手术组、模型组和壮骨止痛方组并提取相应含药血清，提取培养原代成骨细胞并添加相应的含药血清继续培养，先后进行碱性磷酸酶、茜素红染色、免疫组化等一系列的指标检测。结果 见大量被染成了蓝紫色的成骨细胞以及橘红色钙化结节；TGF-β、Smad蛋白在壮骨止痛方组中表达水平明显高于空白对照组和假手术组，模型组中的蛋白表达水平最低，具有统计学意义（P<0.05）。结论 壮骨止痛方可以通过TGF-β-Smad信号转导通路治疗绝经后骨质疏松症并可大量提高该通路的表达水平。 |
| 关键词: 绝经后骨质疏松症 TGF-β-Smad 壮骨止痛方 |
| DOI：10.3969/j.issn.1674-070X.2016.07.007 |
| Received:March 24, 2016 |
| 基金项目:2014年湖南省教育厅科学研究优秀青年项目（14B130）；2015年度湖南省大学生研究性学习和创新性实验计划项目（2015217，2015220）；2016年度湖南省中医药科研计划重点项目（201612）。 |
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| Effect of Zhuanggu Zhitong Decoction on the Treatment of Postmenopausal Osteoporosis based on TGF-β-Smad Pathway |
| LIU Pingan,DAI Yuping,MENG Xiaosha,LIU Shiyu,GAO Fan,ZHANG Guomin |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To explore the Zhuanggu Zhitong prescription on postmenopausal osteoporosis by TGF-β-Smad pathway,and to explore its mechanism.Methods Sixty female rats were randomly divided into blank control group,sham operation group,model group and Zhuanggu Zhitong Fang group and extract the corresponding serum,the primary into bone cells were extracted and cultured,and added with the corresponding serum to culture.A series of indexes on the cells,such as alkaline phosphatase,alizarin red staining and immunohistochemical staining were determined.Results A lot of cells were dyed blue purple and with orange red nodules of calcification.The TGF-β and Smad protein expression in Zhuanggu Zhitong Fang group was significantly higher than those in the blank control group and sham operation group.The protein expression level in model group was the lowest,with statistical significance (P<0.05).Conclusion Zhuanggu Zhitong prescription can treat postmenopausal osteoporosis by TGF-β-Smad signaling transduction pathway and show a significant increase in the expression level of the pathway. |
| Key words: postmenopausal osteoporosis TGF-β-Smad Zhuanggu Zhitong decoction |
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