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黄正新,黄献平,袁肇凯.养心通脉方对大鼠心肌缺血损伤模型心肌细胞线粒体功能的影响[J].湖南中医药大学学报英文版,2016,36(7):25-27.[Click to copy
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| 养心通脉方对大鼠心肌缺血损伤模型心肌细胞线粒体功能的影响 |
| 黄正新,黄献平,袁肇凯 |
| (海南省农垦三亚医院, 海南 三亚 572000;湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 研究养心通脉方对异丙肾上腺素诱导的大鼠心肌缺血损伤模型心肌细胞线粒体呼吸功能的影响。方法 实验大鼠被随机分为3组（每组15只）：对照组、模型组、养心通脉方组，实验结束后所有动物采用脱颈椎法处死，通过测定呼吸控制率（RCR）、二磷酸腺苷磷/氧（ADP/O）比值及ATP的浓度来评价线粒体的呼吸功能，并对线粒体内胆固醇、磷脂（PL）、甘油三酯（TG）和游离脂肪酸（FFAs）的含量进行测定。结果 模型组中大鼠心肌细胞线粒体内RCR、ADP/O比值及ATP浓度均明显低于对照组（P<0.001），而在养心通脉方组中心肌细胞线粒体内RCR、ADP/O比值及ATP浓度均接近于对照组（P>0.05）。与对照组相比，模型组中胆固醇、TG和FFAs的水平均明显升高（P<0.001），同时磷脂（PL）水平明显降低（P<0.001）。但在养心通脉方组中心肌细胞线粒体内胆固醇、TG、FFAs以及PL的水平均与对照组无明显差异（P>0.05）。结论 养心通脉方对异丙肾上腺素诱导的大鼠心肌缺血损伤模型心肌细胞线粒体的呼吸功能及其膜完整性具有保护作用。 |
| 关键词: 养心通脉方 心肌缺血 心肌细胞 线粒体功能 呼吸控制率 二磷酸腺苷磷/氧比值 |
| DOI：10.3969/j.issn.1674-070X.2016.07.005 |
| Received:January 19, 2016 |
| 基金项目:国家自然科学基金项目（81273670）；湖南省教育科学“十二五”规划课题资助（XJK011BGD011）；中医诊断学国家重点学科开放项目（2013ZYZD16）。 |
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| Effect of Yangxin Tongmai Formula on Mitochondrial Function of Cardiomyocyte in Myocardial Ischemia Injury Rat Models |
| HUANG Zhengxin,HUANG Xianping,YUAN Zhaokai |
| (Hainan Province Nongken Sanya Hospital, Sanya, Hainan 527000, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To evaluate the effect of Yangxin Tongmai Fang on mitochondrial respiration of cardiomyocyte in the isoproterenol-induced myocardial ischemia injury rats model.Methods The experimental rats were divided into three groups:control group,model group,Yangxin Tongmai Fang group,15 rats in each group.After the experimental period,the animals were sacrificed by cervical decapitation.The mitochondrial respiration of cardiomyocyte was evaluated by the levels of respiratory control ratio (RCR),ADP/O ratio and ATP concentration.The mitochondrial cholesterol,phospholipids (PL),triacylglycerol (TG) and free fatty acids (FFAs) were also determined.Results The levels of RCR,ADP/O ratio and ATP concentration in model group were lower than that in control group (P<0.001),the RCR,ADP/O ratio and ATP concentration in Yangxin Tongmai group were close to the control group (P>0.05).Compared with the control group,the levels of cholesterol,TG and FFAs in model group were significantly increased (P<0.001),and the PL content was decreased obviously (P<0.001).But there were no significant difference in the levels of cholesterol,TG,FFAs and PL between Yangxin Tongmai Fang group and control Group (P>0.05).Conclusion Yangxin Tongmai Fang shows protective effect on mitochondrial respiration and its membrane integrity of cardiomyocyte in the isoproterenol-induced myocardial injury rat models. |
| Key words: Yangxin Tongmai Fang myocardial ischemia cardiomyocyte mitochondrial function respiratory control rate ADP/O ratio |
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