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张亚利,谢玲玲,欧阳娴,樊悦,罗娟,童巧珍,刘峰.辣椒VQ基因家族的鉴定与低温胁迫下的表达分析[J].湖南中医药大学学报英文版,2016,36(7):11-19.[Click to copy
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| 辣椒VQ基因家族的鉴定与低温胁迫下的表达分析 |
| 张亚利,谢玲玲,欧阳娴,樊悦,罗娟,童巧珍,刘峰 |
| (湖南中医药大学药学院, 湖南 长沙 410208;湖南省农业科学院西瓜甜瓜研究所 湖南 长沙 410125;湖南省农业科学院蔬菜研究所 湖南 长沙 410125) |
| 摘要: |
| 目的 从辣椒（Capsicum annuum L.）全基因组中鉴定VQ基因，并进行基因结构、基因进化、染色体定位以及组织表达和低温诱导表达分析。方法 利用辣椒基因组数据库，通过生物信息学方法，鉴定辣椒基因（CaVQ）家族成员；利用MEME、GSDS 2.0、DNAMAN8、MapInspect等软件进行基因结构及染色体定位分析；采用MEGA6.0软件进行系统进化树分析；利用RNA-seq数据及qRT-PCR的方法进行辣椒组织表达模式和低温胁迫分析。结果 辣椒全基因组中含有29个CaVQ基因；CaVQ基因结构相对简单，不均匀地分布在辣椒各染色体上；CaVQ基因家族分为2组（GroupⅠ和GroupⅡ）及6个亚组（GroupⅠa、GroupⅠb、GroupⅠc、GroupⅡa、GroupⅡb和GroupⅡc）；不同组织中CaVQ基因的表达量具有一定差异，低温胁迫可抑制或激活部分CaVQ基因表达量。结论 CaVQ基因家族包括29个成员，分为2组，6个亚组，分布于10条染色体上，其组织表达模式及基因响应具有多样性。 |
| 关键词: 辣椒 VQ基因家族 鉴定 低温胁迫表达分析 |
| DOI：10.3969/j.issn.1674-070X.2016.07.003 |
| Received:March 28, 2016 |
| 基金项目:国家自然科学基金项目（31470105）；湖南省自然科学基金项目（2015JJ2089）。 |
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| Identification and Low Temperature-induced Expression Analysis of VQ Gene Family in Pepper |
| ZHANY Yali,XIE Lingling,OUYANG Xian,FAN Yue,LUO Juan,TONG Qiaozhen,LIU Feng |
| (School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Institute of Watermelon and Melon, Hunan Academy of Agricultural Sciences, Changsha, Hunan 410125, China;Institute of Vegetable, Hunan Academy of Agricultural Sciences, Changsha, Hunan 410125, China) |
| Abstract: |
| Objective To identify VQ genes from pepper genome,and analyze the gene structure,phylogeny,chromosome location,tissue expression pattern and low temperature-induced expression of CaVQ genes.Methods The CaVQ genes were identified by pepper genome database and bioinformatic method.Gene structure and chromosome localization were analyzed by softwares,such as MEME,GSDS 2.0,DNAMAN 8,MapInspect etc.A phylogenetic tree was created by using the MEGA 6.0 program.Tissue expression pattern and expression under low temperature stress of CaVQ genes were analyzed based on RNA-seq database and qRT-PCR method.Results 29 CaVQ genes were identified from pepper.The structure of CaVQ genes were relatively simple,and distributed on different chromosomes unevenly.CaVQ genes were divided into 2 groups (Group I and Group Ⅱ) and 6 subgroups (Group I a,Group I b,Group I c,Group Ⅱ a,Group Ⅱ b and Group Ⅱc).The expression of this gene family had tissue specificity,and the low temperature stress could change the expression of partial CaVQ genes.Conclusion CaVQ gene family consisted of 29 genes.They were devided into 2 groups,6 subgroups and distributed on 10 chromosomes with special response to external stimulates and different expression patterns in different tissues and developmental stages. |
| Key words: pepper VQ gene family identification low temperature-induced expression analysis |
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