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余娜,马思静,朱克俭.臭牡丹总黄酮对A549细胞上皮间质转化相关蛋白的影响[J].湖南中医药大学学报英文版,2016,36(5):10-13.[Click to copy
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| 臭牡丹总黄酮对A549细胞上皮间质转化相关蛋白的影响 |
| 余娜,马思静,朱克俭 |
| (湖南中医药大学, 湖南 长沙 410208;湖南省中医药研究院, 湖南 长沙 410006) |
| 摘要: |
| 目的 观察臭牡丹总黄酮对A549肺腺癌细胞上皮间质转化（epithelial-mesenchymal transition，EMT）相关蛋白的影响。方法 构建β-catenin真核过表达载体并转染A549细胞，将细胞分为空载组、β-catenin过表达组、空载组+臭牡丹总黄酮组、β-catenin过表达+臭牡丹总黄酮组。MTT法检测不同浓度的臭牡丹总黄酮对A549细胞体外增殖的抑制作用。实时定量PCR检测各组β-catenin mRNA的表达，Western Blot检测各组β-catenin、E-Cardherin 、Vimentin的蛋白水平。结果 MTT显示A549细胞活力随臭牡丹总黄酮浓度的增加受到明显抑制，IC50为2.1 mg/mL。β-catenin 过表达组较空载组的β-catenin、Vimentin表达均明显上调，E-cardherin 表达下调。臭牡丹总黄酮在空载组与β-catenin 过表达组均能明显降低β-catenin mRNA与蛋白水平，增加E-cardherin蛋白表达，略微降低Vimentin蛋白表达。结论 臭牡丹总黄酮对A549肺癌细胞具有一定的体外抑制增殖作用，其抗肿瘤作用的可能机制是通过调控EMT的相关蛋白从而逆转β-catenin诱导的EMT现象而起作用。 |
| 关键词: 臭牡丹总黄酮 上皮间质转化 β-catenin过表达载体 A549细胞 |
| DOI：10.3969/j.issn.1674-070X.2016.05.003 |
| Received:January 25, 2016 |
| 基金项目:湖南省自然科学基金项目（14JJ4066）。 |
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| Effect of the Total Flavonoid of Clerodendron bungei Steud on the Epithelial Mesenchvmal Transition Related Proteins in A549 Cells |
| YU Na,MA Sijing,ZHU Kejian |
| (Hunan University of Chinese medicine, ChangSha, Hunan 410208, China;Hunan Academy of Chinese Medicine, Changsha, Hunan 410006, China) |
| Abstract: |
| Objective To observe the effect of the total flavonoid of Clerodendron bungei Steud (CMD) on the epithelial mesenchvmal transition related proteins in A549 cells. Methods Building the β-catenin overexpression vector and transfected A549 cells. The cells can be divied into empty vector group, β-catenin overexpression group, empty vector group+CMD group, β-catenin overexpression+CMD group. The proliferation inhibition of the total flavonoid of Clerodendron bungei Steud with different concentrations on A549 cells in vitro was detected by MTT method. The expression of β-catenin mRNA was determined with real-time PCR, and the protein expression of β-catenin, E-Cardherin, Vimentin was detected with Western Blot. Results MTT showed that the A549 cells vitality were significantly inhibited with the increasing concentrations of the CMD, the IC50 was 2.1mg/mL. Compared with the empty vector group, the expression of β-catenin and vimentin was significantly higher, the E-cardherin was lower in β-catenin overexpression group. The total flavonoid of Clerodendron bungei Steud can reduce the expression of β-catenin, increase the expression of E-cardherin, and slightly reduce the expression of Vimentin protein in empty vehicle and the β-catenin overexpression groups. Conclusion the total flavonoid of Clerodendron bungei Steud has a certain proliferation inhibition effect on A549 lung cancer cells in vitro. The possible mechanisms is that, it can regulate some related proteins to reverse the EMT phenomenon through β-catenin cell signaling. |
| Key words: the total flavonoid of Clerodendron bungei Steud the epithelial mesenchvmal transition β-catenin expression vector A549 cell |
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