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石金敏,徐梦菲,周灿,邹青伟,张晓燕.不同组织器官中华蛇根草绿原酸含量的比较研究[J].湖南中医药大学学报英文版,2016,36(4):28-30.[Click to copy
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| 不同组织器官中华蛇根草绿原酸含量的比较研究 |
| 石金敏,徐梦菲,周灿,邹青伟,张晓燕 |
| (湖北医药学院附属人民医院药学部, 湖北 十堰 442000;武汉大学第二临床学院, 湖北 武汉 430000) |
| 摘要: |
| 目的 比较中华蛇根草不同组织器官中绿原酸的含量。方法 选用Inertsil C18色谱柱，以甲醇-0.02%磷酸溶液（20：80）为流动相，流速为1.0 mL/min，检测波长为325 nm，柱温40℃。结果 绿原酸在0.027~1.700 μg（r=0.999 9）范围内线性关系良好。2月花期采收的中华蛇根草茎、叶、花中绿原酸的含量分别为3.39、0.77、22.79 mg/g。结论 中华蛇根草不同组织器官绿原酸含量差异较大，其中花含量最高。该方法能准确、快速地进行定性定量检测，可用于中华蛇根草中绿原酸含量的测定。 |
| 关键词: 中华蛇根草 绿原酸 高效液相色谱法 |
| DOI：10.3969/j.issn.1674-070X.2016.04.007 |
| Received:September 09, 2015 |
| 基金项目:湖北省高等学校创新能力提升计划“武当山区道地药材产业化关键技术湖北协同创新中心”鄂教科函[2012]56号。 |
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| Comparative Study on the Content of Chlorogenic Acid in Ophiorrhiza chinensis from Different Organs |
| SHI Jinmin,XU Mengfei,ZHOU Can,ZOU Qingwei,ZHANG Xiaoyan |
| (Department of Pharmacy, Renmin Hospital Affiliated to Hubei University of Medicine, Shiyan, HuBei 442000, China;Zhongnan Hospital of Wuhan University, Wuhan, HuBei 430000, China) |
| Abstract: |
| Objective To determine the content of chlorogenic acid in different organs of Ophiorrhiza chinensis. Methods The contents of chlorogenic acid was determined with chromatographic column Inertsil C18, And its mobile phase was methanol:0.02% phosphoric acid solution (20:80), the flow rate was 1.0 mL/min, detection wavelength was 325 nm and the column temperature was 40℃. Results There was a good linear relationship of chlorogenic acid between 0.027 and 1.700 μg (r=0.999 9). The content of chlorogenic acid of Japanese snakeroot which were harvested during February flowering period, in stems, leaves, flowers were 3.39, 0.77, 22.79 mg/g, respectively. Conclusion The contents of chlorogenic acid, which are also different in different organs of the Ophiorrhiza chinensis, the highest of which is the flower. The method is accurate and quick in qualitative identification and quantitative assay, which can be used for the quality control of Ophiorrhiza Chinensis. |
| Key words: HPLC Ophiorrhiza chinensis chlorogenic acid |
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