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王小妹,陈颂,陈滢宇,胡燕,张晓宇,宁兴旺,朱惠斌.中药复方提取物HNA-1对SIV慢性感染恒河猴胸腺输出及胸腺细胞发育的影响[J].湖南中医药大学学报英文版,2016,36(4):6-10.[Click to copy
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| 中药复方提取物HNA-1对SIV慢性感染恒河猴胸腺输出及胸腺细胞发育的影响 |
| 王小妹,陈颂,陈滢宇,胡燕,张晓宇,宁兴旺,朱惠斌 |
| (湖南中医药大学第一附属医院医学检验中心, 湖南 长沙 410007;广州中医药大学热带医学研究所, 广东 广州 510405;湖南省第二人民医院手足外科, 湖南 长沙 410007) |
| 摘要: |
| 目的 探讨中药复方提取物湘A-1号方（HNA-1，湖南省艾滋病防治小组推荐免费使用的两个中药复方之一的提取物）治疗HIV-1感染及艾滋病（Acquired Immune Deficiency Syndrome，AIDS）的疗效机制。方法 用8只已感染猴免疫缺陷病毒SIVmac239 16～21个月的中国恒河猴，治疗组采用2个月的HNA-1治疗；对照组给予等体积生理盐水灌胃。在预设时间点采集外周血提取DNA，采用定量PCR检测T细胞受体重排删除环（T cell receptor excision circles，TRECs）来评价胸腺输出功能；并用酶消化法分离胸腺细胞，利用流式细胞术检测胸腺内CD34+前体细胞、CD4+T细胞发育各阶段的比例改变。结果 与模型对照组比较，HNA-1治疗组胸腺细胞输出明显增加（P<0.05）；且胸腺内CD34+Lin-细胞比例明显增加（P<0.05），胸腺双阳性T细胞（Double positive，DP）Ⅱ阶段比例减少，而DPⅢ和DPⅣ阶段比例均增加。结论 HNA-1对艾滋病的疗效可能与其能显著增加胸腺输出有关；其作用机制可能涉及增加胸腺内前体细胞数量、以及促进胸腺细胞细胞从DPⅡ阶段发育至DP Ⅲ等。 |
| 关键词: 湘A-号方 CD4+T细胞 胸腺输出 胸腺细胞发育 |
| DOI：10.3969/j.issn.1674-070X.2016.04.002 |
| Received:November 03, 2015 |
| 基金项目:国家自然科学基金资助项目（81274169）；湖南省高等学校科学研究重点项目（12A102）；湖南省教育厅项目（13C688）；湖南省高校科技创新团队“感染性疾病中医药防治研究”资助项目（NO：15）。 |
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| Effects of HNA-1 Extracted from a Chinese Medicine Compound on the Thymic Output and Thymocyte Development in Rhesus Macaques with SIV Chronic Infection |
| WANG Xiaomei,CHEN Song,CHEN Yingyu,HU Yan,ZHANG Xiaoyu,NING Xingwang,ZHU Huibin |
| (The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;The Research Institute of Tropical Medicine of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510403, China;Department of Surgery, the second People's Hospital of Hunan Province, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To investigate the effect of Chinese herbal extract HNA-1 on HIV-1 infection and AIDS. Methods Eight Chinese rhesus macaques had been infected by SIVmac239 for 16 to 21 months were enrolled into this study, the treatment group received HNA-1, control group received normal saline for consecutively 2 months, respectively. In predesigned time-points, peripheral blood were drawn for DNA extraction, and thereby thymic output was evaluated by T-cell receptor excision circle (TRECs) quantification. The thymocyte cells were seperated by enzyme digestion, the proportions of CD34+ cells and thymocytes in various development stages were analyzed by flow cytometry. Results Compared with control group, the thymic output function obviously was elevated in HNA-1 group (P<0.05). And the proportion of CD34+Lin- cells was significantly increased (P<0.05). More interestingly, the thymocytes in double positive(DP) stageⅡdecreased dramatically, while thymocytes in stage DP Ⅲ and stage DP Ⅳ increased. Conclusion Enhanced thymic output function may contribute to the effect of HNA-1 on AIDS treatment with underlying mechanisms involving increased thymic progenitor cells and promoted development of thymocytes from stage DPⅡ to stage DP Ⅲ. |
| Key words: HNA-1 CD4+T cells thymic output thymocyte development |
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