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周兴,周青,赖永金.肾虚肝郁证迟发性性腺功能减退症大鼠模型的建立与评价[J].湖南中医药大学学报英文版,2016,36(3):30-35.[Click to copy
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| 肾虚肝郁证迟发性性腺功能减退症大鼠模型的建立与评价 |
| 周兴,周青,赖永金 |
| (湖南中医药大学第一附属医院, 湖南 长沙 410007;萍乡市中医院, 江西 萍乡 337000) |
| 摘要: |
| 目的 建立“肾虚肝郁证”迟发性性腺功能减退症（Late onset hypogonadism，LOH）动物模型，并对其进行评价。方法 以中医理论“房劳过度伤肾”、“郁久伤肝”为指导，采用“退役种鼠（40周龄）+复合情志刺激+孤养”方法，建立“肾虚肝郁证”LOH动物模型，与正常组（8周龄）比较，以血清总睾酮、悬尾实验、睾丸间质细胞超微结构、睾酮合成相关酶以及Caspase-3 mRNA和蛋白表达为指标。结果 模型组大鼠出现一系列典型的LOH精神、心理、体能改变，与正常组比较，模型组大鼠血清总睾酮水平明显降低，悬尾不动时间显著延长，睾丸组织Caspase-3 mRNA和蛋白表达增强，睾酮合成相关酶类固醇激素合成急性调节蛋白（Steroidogenic Acute Regulatory Protein，StAR）[13]、细胞色素胆固醇侧链裂解酶（Cytochrome P450 side-chain cleavage，P450scc）[14]、3β-羟甾脱氢酶（3beta-hydroxysteroid dehydrogenase，3β-HSD）mRNA和蛋白表达下降，差异均有统计学意义（P<0.01）；模型组睾丸间质变少，睾丸间质细胞线粒体数量减少，出现水肿，线粒体嵴消失。结论 “退役种鼠+复合情志刺激+孤养”方法复制“肾虚肝郁证”LOH模型切实可行，有较高的实用价值。 |
| 关键词: 迟发性性腺功能减退症 动物模型 肾虚肝郁证 |
| DOI：10.3969/j.issn.1674-070X.2016.03.009 |
| Received:December 02, 2015 |
| 基金项目:国家自然科学基金青年科学基金资助项目（81202706）。 |
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| Establishment and Evaluation of Rat Model of Late Onset Hypogonadism of Liver Constraint and Deficiency of Kidney-type |
| ZHOU Xing,ZHOU Qing,LAI Yongjin |
| (The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha Hunan 410007, China;Pingxiang Chinese Medicine Hospital, Pingxiang, Jiangxi 337000, China) |
| Abstract: |
| Objective To establish late onset hypogonadism (LOH) rat models with kidney-asthenia and liver depression syndrome and evaluate their value in research. Methods The rat models with kidney-asthenia and liver depression syndrome were established by using retired mated rat (40 weeks)+various emotional stimulation+ social isolation methods, which is guided by TCM theory "sexual excess injury kidney essence" and "depressed anger damaging the liver". Compared with the control group (8 weeks), the serum testosterone, tail suspension test, Leydig cells ultrastructure, the expression of testosterone synthetase and mRNA and protein of Caspase-3 were observed. Results A series of spirit, psychological health and physical fitness on LOH were observed. Compared with the normal group, serum testosterone levels in the model rats were decreased significantly, TST times increased significantly, the expressions of Caspase-3 were increased, the related testosterone synthetase StAR, P450scc, 3β-HSD were decreased, the differences were statistically significant (P<0.01). Also the testis interstitium was less, the quantity of Leydig cells mitochondrion was reduced and the mitochondrial cristae in the model group was disappeared. Conclusion The LOH rat models with kidney-asthenia and liver depression syndrome can be established by using retired mated rat (40 weeks)+various emotional stimulation+ social isolation methods, which are with high practical values. |
| Key words: late onset hypogonadism models animals kidney-asthenia and liver depression syndrome |
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