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吴琨鹏,易健,彭千元,汤艳,黄昕,刘柏炎.六味地黄汤对快速衰老小鼠认知功能及Wnt3a、GSK-3β表达的影响[J].湖南中医药大学学报英文版,2016,36(3):9-13.[Click to copy
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| 六味地黄汤对快速衰老小鼠认知功能及Wnt3a、GSK-3β表达的影响 |
| 吴琨鹏,易健,彭千元,汤艳,黄昕,刘柏炎 |
| (湖南中医药大学, 湖南 长沙 410208;益阳医学高等专科学校, 湖南 益阳 413000) |
| 摘要: |
| 目的 研究六味地黄汤对D-半乳糖（D-gal）致快速衰老小鼠认知功能及Wnt3a、GSK-3β表达的影响。方法 以500 mg/（kg·d）的D-半乳糖颈部皮下注射复制快速衰老小鼠模型，将动物随机分为正常组，模型组，六味地黄（简称六味）高、中、低剂量组，VitE组，给予相应干预，历时8周。观察小鼠的体质量变化，采用Morris水迷宫实验评价小鼠的认知功能，免疫组化法、RT-qPCR法检测Wnt3a、GSK-3β的表达。结果 六味地黄汤对快速衰老小鼠体质量及水迷宫实验的影响：模型组与正常组比较，差异具有显著统计学意义（P<0.01）；各用药组与模型组比较，差异具有显著统计学意义（P<0.01）；六味高剂量组与其他用药组比较，差异具有统计学意义（P<0.05）；六味地黄汤对快速衰老小鼠认知功能及Wnt3a、GSK-3β表达的影响：模型组与正常组比较，差异具有显著统计学意义（P<0.01），各用药组与模型组比较，差异具有显著统计学意义（P<0.01），六味高剂量组与其他用药组比较，差异具有显著统计学意义（P<0.01）。结论 六味地黄汤可以有效改善D-半乳糖致快速衰老小鼠的学习记忆障碍；增加衰老小鼠Wnt3a的表达，激活Wnt信号通路，下调GSK-3β的表达可能是六味地黄汤延缓衰老健脑益智的作用机制。 |
| 关键词: 六味地黄汤 认知功能 Wnt3a GSK-3β 抗衰老 熟地黄 山药 山茱萸 |
| DOI：10.3969/j.issn.1674-070X.2016.03.003 |
| Received:September 11, 2015 |
| 基金项目:国家自然科学基金项目（81202694），湖南省自然科学基金项目（2015JJ2105）。 |
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| Effects of Liuwei Dihuang Decoction on Recognition and the Expression of Wnt3a and GSK-3β in Senescence Accelerated Mice |
| WU Kunpeng,YI Jian,PENG Qianyuan,TANG Yan,LIU Baiyan |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Yiyang Medical College, Yiyang, Hunan 413000, China) |
| Abstract: |
| Objective To study the effect of Liuwei Dihuang Decoction (LWDHD) on recognition, Wnt3a and GSK-3β in D-galactose induced senescence accelerated mice. Methods The senescence accelerated mice were injected 500 mg/(kg·d) of D-galactose subcutaneously copy rapid, the animals were randomly divided into normal group, model group, high dose, medium dose and low dose of LWDHD groups and VitE group, which were given different processing. After gavage 10 mL/(Kg·d) for eight weeks, the mice body quality change was changed, cognitive function in mice was evaluated by line Morris water maze experiment, the expression of Wnt3a and GSK-3β were detected by immunohistochemical and RT-qPCR methods. Results Compared with normal group and other groups, the weight gain of body mass in model group was lesser (P<0.05), the incubation period was longer and cross platform times was more (P<0.05); Compared with normal group, the diffrences of the body quality increases, the incubation period and cross platform number in high dose of LWDHD group were not statistically significant (P>0.05); Compared with VitE group, the body quality and the ability of learning and memory in low dose of LWDHD group have no statistical significance (P>0.05); The high dose of LWDHD group and other groups were statistically significant (P<0.05). The result showed high consistency by immunohistochemical method and RT-qPCR detection. Compared with the model group, the low expression of Wnt3a, high activity of GSK-3β in high dose of LWDHD group, the difference was statistically significant (P<0.01); Compared with the medium and low dose of LWDHD groups and VitE group, the high expression of Wnt3a and low activity of GSK-3β in the high dose of LWDHD group were statistically significant (P<0.01). Conclusion Liuwei Dihuang Decoction can improve the learning and memory dioders in D-galactose induced senescence accelerated mice. The mechanism of Liuwei Dihuang Decoction in anti-aging and promoting intelligence could be increasing the Wnt3a expression in aging mice, activation of Wnt signaling pathways, and down-regulating GSK-3β expression. |
| Key words: Liuwei Dihuang Decoction cognitive function Wnt3a GSK-3β anti-aging Radix Rehmanniae Preparata Rhizoma Dioscoreae Fructus Corni |
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