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陈青扬,黄惠勇,刘卜菡,曾光,潘继兴.加味生脉补心丹对防治2型糖尿病大鼠心肌损害的影响[J].湖南中医药大学学报英文版,2016,36(3):5-8.[Click to copy
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| 加味生脉补心丹对防治2型糖尿病大鼠心肌损害的影响 |
| 陈青扬,黄惠勇,刘卜菡,曾光,潘继兴 |
| (湖南中医药大学中医诊断, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察加味生脉补心丹对2型糖尿病大鼠模型血糖及心肌组织结构的影响，初步探讨加味生脉补心丹对糖尿病心肌纤维化和肥大的防治作用。方法 40只SD大鼠随机分为空白组7只，实验组33只，通过高糖高脂饲料+小剂量STZ构建2型糖尿病大鼠模型。将成模后的实验组大鼠随机分为模型组、补心丹（BXD）组和罗格列酮（RSG）组各11只，BXD组以浓缩后加味生脉补心丹汤剂灌胃；RSG组按照罗格列酮钠溶液灌胃。给药8周后测各组大鼠空腹血糖，并处死取材，光镜观察心肌组织变化，Masson染色观察纤维沉积情况，免疫组化法观察Ⅰ、Ⅲ型胶原蛋白和沉默信息调节因子3（Sirt3）蛋白的表达。结果 成模时各实验组空腹血糖（FPG）均较空白组升高（P<0.05），造模成功。治疗完成时，RSG组、BXD组FPG均较模型组下降（P<0.05），RSG组FPG较BXD组更低（P<0.05）。模型组心肌组织破坏严重，胶原纤维含量增多，RSG组和BXD组心肌组织破坏程度较轻，胶原纤维含量较少。模型组Ⅰ、Ⅲ型胶原和Sirt3的表达明显高于其他组（P<0.05）。BXD组Ⅲ型胶原的表达明显高于空白组（P<0.05）。RSG组Sirt3的表达显著高于BXD组（P<0.05）。结论 加味生脉补心丹能够明显降低2型糖尿病大鼠的血糖，并能够明显减轻心肌纤维化、肥大的程度，延缓糖尿病所致心肌病的进程。 |
| 关键词: 加味生脉补心丹 2型糖尿病 Ⅰ型胶原蛋白 Ⅲ型胶原蛋白 沉默信息调节因子3 心肌损害 太子参 黄芪 |
| DOI：10.3969/j.issn.1674-070X.2016.03.002 |
| Received:December 17, 2015 |
| 基金项目:国家自然科学基金（81373551）；教育部博士点博士导师基金（2013432311001）；湖南省科技厅重点项目（S2014S2032）；中医诊断国家重点学科开放基金（2013ZYZD04，2013ZYZD27，2014ZYZD05，2014ZYZD28） |
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| Therapeutic Effect of Modified Shengmai Buxin Pills on the Myocardial Damage in Type 2 Diabetes Rats |
| CHEN Qingyang,HUANG Huiyong,LIU Bohan,ZENG Guang,PAN Jixing |
| (Institute of TCM Diagnosis, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effect of modified Shengmai Buxin pills on the FPG and myocardial structure in type 2 diabetes rats, and preliminary investigate the therapeutic effect on myocardial fibrosis and hypertrophy. Methods 40 SD rats were randomly distributed into the blank group with 7 rats and experimental group with 33 rats. The type 2 diabetes models were established by feeding the animals with high-fat and high-sucrose diet. The establisged model rats in experimental group were randomly divided into the model group, Buxindan group (BXD) and rosiglitazone group (RSG) group, 11 rats in each group. The BXD group was given concentrated modified Shengmai Buxindan decoction by gavage and the RSG group was with rosiglitazone sodium. After 8 weeks, FPG of rats was measured and excuted for heart sample. The changes of myocardial tissue were observed. The fibrous deposition was observed by masson method. The expression of collagen I/Ⅲ and silent information regulator-3 (Sirt3) was determined by immunohistochemical method. Results The FPG of experimental group was higher than normal group (P<0.05). The FPG of RSG group and BXD group was higher than model group (P<0.05). The FPG of RSG group was lower than BXD group (P<0.05). The myocardial structure of model group was severely damaged and collagen fiber content increased obviously. The damage of the myocardial structure was slighter and the content of collagen was lower in RSG group and BXD group. Compared with the model group, the expression level of collagen I/Ⅲ and SIRT3 were decreased significantly (P<0.05) in the other groups. Compared with the normal group, the expression level of collagen Ⅲ was increased significantly (P<0.05) in the BXD group. The expression level of Sirt3 in RSG group was increased significantly than the RSG group (P<0.05). Conclusion The modified Shengmai Buxin pills can significantly decrease the level of blood sugar in type 2 diabetes rats. It can alleviate the degree of myocardial fibrosis and hypertrophy, and delay the progression of diabetic cardiomyopathy. |
| Key words: modified Shengmai Buxin pills type 2 diabetes collagen I collagen III silent information regulator-3 myocardial damage Radix Pseudostellariae Astragalus membranaceus |
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