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陈娉婷,周小青,曹泽标,陈昱文,刘旺华,李花.丹龙醒脑方对脑缺血再灌注大鼠海马区Notch信号通路相关蛋白表达的影响[J].湖南中医药大学学报英文版,2016,36(1):10-14.[Click to copy
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| 丹龙醒脑方对脑缺血再灌注大鼠海马区Notch信号通路相关蛋白表达的影响 |
| 陈娉婷,周小青,曹泽标,陈昱文,刘旺华,李花 |
| (湖南中医药大学中医诊断学科, 湖南 长沙 410208;中医诊断学湖南省重点实验室, 湖南 长沙 410007;湖南中医药大学中医诊断学科, 湖南 长沙 410208;数字中医药协同创新中心, 湖南 长沙 410208) |
| 摘要: |
| 目的 探讨丹龙醒脑方对脑缺血再灌注模型大鼠海马区Notch信号通路相关蛋白Notch1、Notch2及Hes1表达的影响。方法 将80只雄性SD大鼠随机分为假手术组、模型组、丹龙醒脑方小剂量组（丹小组7.4 g/kg）、大剂量组（丹大组14.8 g/kg），线栓法建立局灶性脑缺血再灌注大鼠模型，再灌注7 d取缺血侧海马组织。采用免疫组织化学法、免疫印迹法检测大鼠海马区Notch1、Notch2及Hes1蛋白表达。结果 与假手术组比较，各组Notch1、Notch2及Hes1蛋白表达显著升高（P<0.05，P<0.01）；与模型组比较，丹小组、丹大组Notch1、Notch2及Hes1蛋白表达显著升高（P<0.05，P<0.01）。结论 丹龙醒脑方能通过上调海马区Notch1、Notch2及Hes1蛋白表达水平，调节Notch信号转导通路，这可能是其促进脑缺血损伤后神经功能修复的机制之一。 |
| 关键词: 丹龙醒脑方 脑缺血 海马区 Notch信号通路 Notch1 Notch2 Hes1 丹参 三七 地龙 |
| DOI：10.3969/j.issn.1674-070X.2016.01.002 |
| Received:September 25, 2015 |
| 基金项目:国家自然科学基金（81373702，81473567，81202632）；教育部博士点基金（20124323120003）；湖南省自然科学基金（13JJ3097）；湖南省教育厅科研项目（14B134，15K092）；湖南中医药大学研究生创新课题（2015CX15）。 |
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| Effects of Danlong Xingnao Recipe on Protein Expressions Related to Notch Signaling Pathway in Hipppcampus of Cerebral Ischemia Reperfusion Injury Model Rats |
| CHEN Pingting,ZHOU Xiaoqing,CAO Zebiao,CHEN Yuwen,LIU Wanghua,LI Hua |
| (Institute of Diagnostics of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The Key Laboratory of Diagnostics of Chinese Medicine in Hunan Province, Changsha, Hunan 410007, China;Institute of Diagnostics of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Collaborative Center for Research and Innovation of Digital Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To investigate the effects of Danlong Xingnao recipe (DLXNR) on the expressions of Notch1, Notch2 and Hes1 protein related to the Notch signaling pathway in hippcampus of cerebral ischemia-reperfusion injury model rats. Methods 80 male SD rats were randomly divided into sham-operation group, model group, Danlong Xingnao recipe small-dose group (Dan-small group 7.4 g/kg) and Danlong Xingnao recipe high-dose group (Dan-high group 14.8 g/kg). The local cerebral ischemia/reperfusion rat model was established by intraluminal thread occlusion of the middle cerebral artery. After reperfusion for 7d, the ischemia hippocampus tissues were taken out. The Notch1, Notch2 and Hes1 protein expressions in hippocampal were determined respectively by immunohistochemicstry and Western Blot method. Results Compared with the sham-operation group, the expression of Notch1, Notch2 and Hes1 protein in other groups were increased obviously (P<0.05, P<0.01). Compared with the model group, the expression of Notch1, Notch2 and Hes1 protein in Dan-small group and Dan-high group were increased obviously (P<0.05, P<0.01). Conclusion Danlong Xingnao recipe can upregulate the expressions of Norch1, Notch2 and Hes1 protein, regulate the Notch signal transduction pathway. It may be one of the mechanisms to promote the repair of brain injury. |
| Key words: Danlong Xingnao recipe brain ischemia hippcampus Notch signaling pathway Notch1 Notch2 Hes1 Salvia miltiorrhiza Panax notoginseng lumbricus |
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