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沈 菁1， 彭 艳1， 史冬梅1， 封迎帅2， 侯艳玲1， 林亚平1*.艾灸对幽门螺杆菌胃炎大鼠血清白介素及T淋巴细胞亚群的影响[J].湖南中医药大学学报,2015,35(10):30-32[Click to copy
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| 艾灸对幽门螺杆菌胃炎大鼠血清白介素及T淋巴细胞亚群的影响 |
| 沈菁1，彭艳1，史冬梅1，封迎帅2，侯艳玲1，林亚平1* |
| (1.湖南中医药大学，湖南 长沙，410208;2.湖南省人民医院， 湖南 长沙，410005) |
| 摘要: |
| 目的 观察艾灸对幽门螺杆菌胃炎模型大鼠胃黏膜组织形态学变化、血清IL-6、IL-8、TNF-α、Hp-IgG、CD3+、CD4+、CD8+的影响，探讨艾灸干预HP胃炎胃粘膜损伤的机制。方法 40只SD大鼠随机分为4组，每组10只，即A组（空白组）、B组（Hp胃炎模型组）、C组（艾灸+模型组）、D组（电针+模型组）。除A组外其余各组大鼠均以NaHCO3+消炎痛+Hp灌胃造模。各组经相应的干预后，HE染色光镜下观察大鼠胃黏膜组织形态学变化，Elisa法测定血清中IL-6、IL-8、TNF-α、Hp-IgG的含量，流式细胞术测CD3+、CD4+、CD8+、 CD4+/CD8+值。结果 与A组相比，B组大鼠血清IL-6、IL-8、TNF-α、Hp-IgG、CD8+明显增高（P＜0.01），CD3+、CD4+、CD4+/CD8+明显降低（P＜0.01）。与B组相比，C组血清IL-8、TNF-α、IL-6含量、Hp-IgG、CD8+明显降低（P＜0.05或0.01），CD3+、CD4+、CD4+/CD8+明显升高（P＜0.05），D组各指标无明显变化（P＞0.05）。与D组相比，C组IL-6、IL-8、Hp-IgG明显降低（P＜0.05），CD4+/CD8+明显升高（P＜0.05）。结论 艾灸防治幽门螺杆菌胃炎的作用可能通过两种途径实现，抑制促炎细胞因子IL-6、IL-8、TNF-α的分泌，促进免疫因子CD3+、CD4+产生，抑制CD8+分泌，且艾灸对胃黏膜组织保护作用比电针更明显。 |
| 关键词: 艾灸 幽门螺杆菌胃炎 IL-6 IL-8 TNF-α Hp-IgG CD3+ CD4+ CD8+ |
| DOI： |
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| 基金项目:国家自然科学基金项目(81072867，81403486) |
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| Effects of Moxibustion on Serum Interleukin and T Lymphocyte Subsets of Helicobacter pylori-associated Gastritis Rats |
| SHEN Jing1, PENG Yan1, SHI Dongmei1, FENG Yingshuai2, HOU Yanling1, LIN Yaping1* |
| (1.Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; 2. People's Hospital of Hunan Province, Changsha, Hunan 410005, China) |
| Abstract: |
| Objective To explore the effects of moxibustion on the morphologic changes of gastric mucosa tissues and expression of serum IL-6, IL-8, TNF-α, Hp-IgG, CD3+, CD4+, CD8+, CD4+/CD8+ in Helicobacter pylori (Hp)-associated gastritis model rats，to reveal the mechanisms underlying the protective effect of moxibustion against gastric inflammatory injury. Methods 40 healthy SD rats were randomly divided into 4 groups：the group A (blank group), group B (Hp model group), group C (moxibustion model groups) and group D (electro-acupuncture model group), 10 rats in each group. The rats except for the group A were modeled by oral gavage with NaHCO3+indomethacin+Hp. After the intervention, the morphologic changes of gastric mucosa tissues in rats were observed by HE staining. The contents of serum IL-6, IL-8, TNF-α, Hp-IgG were detected by ELISA, and Contents of CD3+, CD4+, CD8+ and CD4+/CD8+ were detected by flow cytometry. Results Compared with group A, the expression of serum IL-6, IL-8, TNF-α, Hp-IgG, CD8+ in group B were significantly increased (P<0.01), and contents of CD3+, CD4+ and CD4+/CD8+ were decreased obviously (P<0.01). Compared with group B, the expression of serum IL-6, IL-8, TNF-α, Hp-IgG, CD8+ in group C were significantly decreased (P>0.05 or 0.01), contents of CD3+(P<0.05)、CD4+ (P<0.05) and CD4+/CD8+ were increased significantly (P<0.05), no significant difference in group D (P>0.05). Compared with group D, the expression of IL-6, IL-8, Hp-IgG in group C were significantly decreased (P>0.05 or 0.01), CD4+/CD8+ increased significantly (P<0.05). Conclusion The mechanisms of moxibustion in treatment of H. pylori-induced gastric mucosal inflammatory injury were probably through two pathways, Inhibition of proinflammatory cytokine IL-6, IL-8, TNF-αsecretion, and promoting immune factor CD3+、CD4+ secretion to inhibit the secretion of CD8+. The protective effect of moxibustion on gastric mucosa tissues was better than that of electro-acupuncture therapy. |
| Key words: moxibustion, Hp-gastritis, IL-6,IL-8,TNF-α,Hp-IgG, CD3+, CD4+, CD8+ |
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