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周红玲;石雕;王智颖;刘阳;丁楠;.高效液相色谱法测定复方咳喘颗粒中盐酸麻黄碱的含量[J].湖南中医药大学学报,2014,34(8):21-23[Click to copy
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| 高效液相色谱法测定复方咳喘颗粒中盐酸麻黄碱的含量 |
| 周红玲;石雕;王智颖;刘阳;丁楠; |
| (湖南中医药高等专科学校附属第一医院) |
| 摘要: |
| 目的建立高效液相色谱法测定复方咳喘颗粒中盐酸麻黄碱含量的方法。方法采用色谱柱为Agilent Extend-C18柱(4.6 mm×250 mm,5μm);流动相为乙腈-0.1%磷酸溶液(含0.1%三乙胺)(4∶96):流速为1 mL/min;柱温为25℃;检测波长为260 nm;进样量为10μL。结果盐酸麻黄碱在0.24~2.4μg内线性关系良好,回归方程Y=4 118X-36.3,r=0.999 8(n=6),平均加样回收率为98.2%,RSD为0.5%(n=6)。结论本法快速简便、重复性好、结果准确,可用于测定复方咳喘颗粒中盐酸麻黄碱的含量。 更多还原 |
| 关键词: 复方咳喘颗粒 盐酸麻黄碱 高效液相色谱法 含量测定 |
| DOI： |
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| 基金项目:湖南中医药管理局资助项目(2013144) |
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| Determination Ephedrine Hydrochloride in Compound Cough Granules by HPLC |
| ZHOU Hongling;SHI Diao;WANG Zhiying;LIU Yang;DING Nan |
| (The First Affiliated Hospital of Hunan Traditional Chinese Medical College) |
| Abstract: |
| [Abstract) Objective To establish a HPLC method for determination of ephedrine hydrochloride
in compound cough granules. Methods Agilent Extend-C18 column (5 N m, 4.6 mmx 250 mm) was
used and the mobile phase was acetonitrile-0.1% phosphoric acid (4:96). The flow rate was
1.0 mL/min, the column temperature was 25 0C and the wavelength was 260 nm. The injection
volume was 10 N L. Results The ephedrine hydrochloride was liner over the range of
0.24-2.49 N g. The regression equation Y} 118.1X-36.278, correlation coefficient }=0.999 8(n=6).
The average recovery was 98.2% with RSD=0.5%(n=6). Conclusions The method is rapid simple,
reproducible, accurate, and can be used for the content determination of ephedrine hydrochloride
in compound cough granules. |
| Key words: compound cough granules phedrine hydrochloride HPLC content determination |
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