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李凤娟； 周日宝； 刘湘丹； 刘笑蓉； 王朝晖； 孙梦姗；.不同采收期菝葜中黄酮类成分含量动态变化的研究[J].湖南中医药大学学报英文版,2012,32(11):38-41.[Click to copy
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| 不同采收期菝葜中黄酮类成分含量动态变化的研究 |
| 李凤娟；周日宝；刘湘丹；刘笑蓉；王朝晖；孙梦姗； |
| (湖南中医药大学) |
| 摘要: |
| 目的以黄酮类含量为指标,确定菝葜根茎药材的最佳采收期。方法以芦丁为对照品,采用紫外-可见分光光度法测定不同采收期菝葜根茎中总黄酮含量,并采用HPLC测定其中落新妇苷和黄杞苷的含量。结果 11月中旬以前总黄酮含量明显低于11月中旬以后;10~11月初随着生育期延长菝葜根茎中总黄酮含量略有下降,11月份以后菝葜根茎中总黄酮含量不断积累,至12月底菝葜进入休眠期后菝葜根茎中总黄酮的含量最高,达3.0%±0.1%。菝葜根茎药材中落新妇苷和黄杞苷的总含量大于0.10%,符合2010版《中华人民共和国药典》的规定,在12月底含量达到最大值。结论菝葜药材的最佳采收期应为菝葜地上部位落叶枯萎,根茎进入越冬休眠期后采收。 |
| 关键词: 菝葜 黄酮类成分 含量测定 紫外-可见分光光度法 HPLC 不同采收期 |
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| 基金项目:湖南省教育厅高校创新平台开放基金(09k061);湖南省中医药管理局(2009010) |
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| Research on dynamic changes of flavonoids contentsin Smilax China L． from different harvest time |
| LI Feng-juan,ZHOU Ri-bao,LIU Xiang-dan,LIU Xiao-rong,WANG Zhao-hui,SUN Meng-shan |
| (TCM University of Hunan) |
| Abstract: |
| 〔Abstract〕Objective To determine the optimum harvest time of Smilax China L using the
flavonoids content as an index． Methods The content of total flavonoids in Smilax china L．
was determined by ultraviolet-visible spectrophotometry with rutin as standard control，and astilbin
and engeletin in Smilax China L． were determined using HPLC method. Results The total
flavonoids content of Smilax China L. harvested before the mid-November is markedly lower than
that of harvested after the mid -November. As the growth period extending，the total
flavonoids content in the rhizome of Smilax China L. gradually reduced from October to the begin
ning of November，but the flavonoids content constantly accumulated after November and reached
the peak when the rhizome of Smilax China L．entered hibernation at the end of December，which is
3.0%±0.1%. The total content of astilbin and engeletin in the rhizome of Smilax China L． was more
than 0.10 %，which complied with the quality standards in the 2010 edition of the China pharma
copoeia，and the content reached the peak at the end of December. Conclusion The Smilax Chi
na L． may be collected at the time that Smilax China L. leaves has fallen and the
above-ground has withered，and the rhizome has entered winter hibernation. |
| Key words: Smilax China L． flavonoids quantitative determination ultraviolet-visible spectrophotometry HPLC different harvest time |
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