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谭洁，滕久祥，彭芝配*，马薇，殷坚.九香止泻片对湿热型泄泻肠易激综合征
模型大鼠AQP4、PKA 与cAMP 表达的影响[J].湖南中医药大学学报英文版,2012,32(7):23-26.[Click to copy
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| 九香止泻片对湿热型泄泻肠易激综合征
模型大鼠AQP4、PKA 与cAMP 表达的影响 |
| 谭洁，滕久祥，彭芝配*，马薇，殷坚 |
| (湖南中医药大学) |
| 摘要: |
| 目的探讨九香止泻片对湿热型泄泻肠易激综合征（IBS）模型大鼠肠道水通道蛋白4（AQP4）、cAMP 依赖
性蛋白激酶（PKA）与环腺苷酸（cAMP）的影响。方法40 只SD 大鼠，随机分为空白组、模型组、九香止泻片组、盐酸洛
哌丁胺对照组，每组10 只, 除空白组外，其余各组大鼠在造模成功后分别给予不同药物或蒸馏水连续灌胃7 d，腹腔
麻醉取血处死后取结肠组织测定大鼠肠道AQP4、PKA 表达与cAMP 含量。结果与空白组比较， 模型组肠道AQP4、
PKA 蛋白表达均显著降低（P＜0.05），cAMP 含量下降，差异有统计学意义（P＜0.05）。与模型组比较，九香止泻片组和盐
酸洛哌丁胺对照组肠道AQP4、PKA 蛋白表达均显著增强（P＜0.05），cAMP 含量显著增加（P＜0.05）; 九香止泻片组和盐
酸洛哌丁胺对照组比较差异无统计学意义（P＞0.05）。结论九香止泻片可能通过激活cAMP-PKA 信号通路调控湿热
型泄泻IBS 模型大鼠肠道中AQP4 的表达进而改善腹泻症状，是治疗湿热型泄泻IBS 的有效方药。 |
| 关键词: 湿热型泄泻 肠易激综合征 九香止泻片 大鼠 |
| DOI： |
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| 基金项目:湖南省教育厅资助项目（09C732）；湖南省教育厅资助项目(10w021)；长沙市科技计划资助项目（k1009002-31）。 |
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| Effects of JXZXP on AQP4， PKAand cAMP expression of irritable bowel syndrome ratswith damp-heat diarrhea type |
| TAN Jie, TENG Jiu-xiang, PENG Zhi-pei, MA Wei， YIN Jian |
| (TCM University of Hunan) |
| Abstract: |
| Objective To investigate the effects of Jiuxiang Zhixie tablet (JXZXP) on AQP4，
PKA and cAMP expression of irritable bowel syndrome (IBS) rats with damp-heat diarrhea type.
Methods 40 SD rats were randomly divided into 4 groups as follows: blank group, model group,
JXZXP group and loperamide hydrochloride positive group, 10 rats in each group. Other mice except
for mice in blank group were given different drugs or distilled water respectively after modeled
(ig 7 d). After treatment, all mice were killed and taken for colon tissues for observations of AQP4,
PKA expression levels and cAMP content. Results Compared with blank control group, the AQP4,
PKA expression levels and cAMP contents of colon in model group were reduced significantly(all
P <0.05). Compared with model group, the AQP4, PKA expression levels and cAMP contentsof colon in JXZXP group and loperamide hydrochloride group were increased significantly (all P<
0.05), But the differences between JXZXP group and loperamide hydrochloride group were no statistically
significant(P＞0.05). Conclusion JXZXP may be improve the diarrhea symptoms of IBS rats
with damp -heat diarrhea type by activating the expression of AQP4 in the intestinal tract of
the cAMP-PKA signaling pathway and is an effective medicine. |
| Key words: TCM damp-heat diarrhea type irritable bowel syndrome JXZXP rat |
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