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刘慧萍； 尤昭玲； 张国民； 刘春燕； 王宏宝； 肖艺； 吴霞； 庄乔； 黄珍琦； 雷磊；.补肾活血方对免疫性卵巢早衰小鼠卵泡凋亡相关蛋白的影响[J].湖南中医药大学学报英文版,2012,32(5):28-31.[Click to copy
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| 补肾活血方对免疫性卵巢早衰小鼠卵泡凋亡相关蛋白的影响 |
| 刘慧萍；尤昭玲；张国民；刘春燕；王宏宝；肖艺；吴霞；庄乔；黄珍琦；雷磊； |
| (湖南中医药大学分子病理与药理实验室; 湖南中医药大学湘杏学院) |
| 摘要: |
| 目的研究补肾活血方对卵巢早衰(POF)小鼠卵泡凋亡调控相关基因的表达,为临床中医药预防和治疗卵巢早衰提供思路。方法采用透明带3作抗原,对BALB/c雌性小鼠皮下多点注射,建立免疫性POF模型。用补肾活血方低、中、高剂量进行治疗,设倍美力为阳性对照药,采用ELISA法测定外周血清抗透明带抗体(AzpAb)的含量,进行脏器指数及卵巢病理学观察,免疫组织化学SABC法检测卵泡凋亡调控基因(bcl-2/Bax)表达变化。结果与空白组比较,模型组AzpAb表达明显增高,差异有统计学意义(P<0.01);与模型组比较,各剂量组、倍美力组表达均明显下降,差异有统计学意义(P<0.01);与空白组比较,模型组卵巢指数、胸腺指数均明显下降,脾脏指数明显增加,差异有统计学意义(P<0.01),与模型组比较,高剂组和倍美力组卵巢指数(P<0.01)、胸腺指数(P<0.05)增加,脾脏指数降低(P<0.05);与空白组比较,模型组bcl-2蛋白表达明显下降、Bax蛋白表达显著增加,差异有统计学意义(P<0.01),与模型组比较,各剂量组、倍美力组bcl-2蛋白表达明显增高(P<0.01),Bax蛋白表达显著降低(P<0.01)。结论补肾活血方可能通过调节凋亡调控基因(bcl-2/Bax)的表达,使AzpAb表达明显下降,从而调节免疫反应,抑制抗体集聚,阻止卵泡的过度凋亡,改善卵巢的免疫功能,使有生机卵泡增加,淋巴细胞、浆细胞浸润减少,达到抑制自身免疫损伤、保护卵巢、改善卵巢功能的目的。 |
| 关键词: 补肾活血方 卵巢早衰 自身免疫 卵泡凋亡 抗透明带抗体 小鼠 补骨脂 紫石英 菟丝子 |
| DOI： |
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| 基金项目:国家自然科学基金资助项目(30973771);中国博士后科研基金(2012M511382);湖南省自然科学基金资助项目(11JJ4075);国家教育部高等学校博士学科点专项科研基金(20114323120007);湖南省大学生研究性学习和创新性实验计划项目(2011-480) |
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| Effect of Bushen Huoxue decoction on follicle apoptosis-related protein of immune premature ovarian failure in mice |
| LIU Hui-ping1,YOU Zhao-lin1,ZHANG Guo-min1,LIU Chun-yan1,WANG Hong-bao1, XIAO Yi1,WU Xia1,ZHUANG Qiao2,HUANG Zhen-qi2,LEI Lei1 |
| (TCM University of Hunan) |
| Abstract: |
| Objective To study the effcts of Bushen Huoxue decoction on premature ovarian failure (POF) of follicle apoptosis-related protein expression in mice and provide ideas for clinical prevention and treatment of POF with Chinese medicine. Methods The zona pellucida 3 as the antigen, the BALB/c female mice, were given multi-point injectionin subcutaneous immunization to induce the models of autoimmune POF. Then the mice were treated with Bushen Huoxue decoction in low-dose, mudium-dose, high-dose and Premarin(as control), respectively. Ovarian index, thymus index, spleen index and the morphology of ovary tissue were studied. The follicle apoptosis-related regulatory protein (Bcl-2/Bax) expression was detected by immunohistochemidtry SABC and serum AzpAb content was measured by ELISA. Result Compared with model group, the ovarian index and thymus index were increased (P<0.01,P<0.05), and spleen index was decreasd(P<0.05) in Bushen Huoxue decoction high-dose group and Premarin group; the ovarian index and thymus index were increased and spleen index was decreased in Bushen Huoxue prescription median-dose group, but there were no significants differences. Bcl-2 protein expression in the control group, Bushen Huoxue prescription all dose groups, Premarin group were significantly higher than that of modeling group (all P<0.05). While Bax protein and AzpAb expressions in the control group, Bushen Huoxue decoction in all dose groups, Premarin group were significantly lower than in model group(P<0.05).Conclusion Bushen Huoxue decoction may influence the apoptosis-related regulatory protein (such as Bcl-2 and Bax) expressions, decrease AzpAb expression to improve ovarian function. |
| Key words: premature ovarian failure Bushen Huoxue decoction autoimmunity apoptosis AzpAb mice Psoralea corylifolia Taxillus chinensis Danser Cuscuta chinensis lam |
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