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翁榕安； 胡劲松； 翁诗玉；.水溶性黑柄炭角菌肽的体外抗氧化活性[J].湖南中医药大学学报英文版,2012,32(3):10-13.[Click to copy
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| This paper
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| 水溶性黑柄炭角菌肽的体外抗氧化活性 |
| 翁榕安；胡劲松；翁诗玉； |
| (湖南师范大学) |
| 摘要: |
| 研究水溶性黑柄炭角菌肽的体外抗氧化活性 方法通过测定水溶性黑柄炭角菌肽对邻苯三酚自
氧化产生的超氧阴离子Fenton反应产生的羟自由基DPPH自由基的清除率还原能力及抑制脂质体过氧化全面
考察水溶性黑柄炭角菌肽的体外抗氧化能力 结果水溶性黑柄炭角菌肽能明显降低邻苯三酚自氧化速率对羟自
由基有极强的清除能力对DPPH自由基清除率随浓度增加而增加其IC50 分别为10 0.49 mg/mL和16.3 g/mL
进一步研究表明黑柄炭角菌肽具有一定的还原力且与浓度呈正比例关系同时也能抑制脂质体的过氧化反应 结
论水溶性黑柄炭角菌肽在体外具有明显的抗氧化作用
关键词 |
| 关键词: 黑柄炭角菌 肽 抗氧化 体外试验 |
| DOI： |
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| 基金项目:湖南省自然科学基金资助项目(10JJ3039) |
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| Anti-oxidative activity of water-soluble peptidesfrom Xylaria nigripes in vitro |
| WENG Rong-an,HU Jin-song,WENG Shi-yu |
| (Hunan Normal university, Changsha, Hunan 410081, China)) |
| Abstract: |
| To study anti-oxidative activity of water-soluble peptides from Xylaria
nigripes (XNP) in vitro. MethodsThe anti-oxidative activity of water-soluble peptides from XNP
were studied entirely by measuring scavenging rate of water-soluble peptides from XNP on superoxide anion generated by pyrogallol autoxidation, hydroxyl free radical in Fenton reaction and
DPPH free radical, and their reducing power and inhabiting peroxidation of liposomes ability. Re
sults Water -soluble peptides from XNP could significantly reduce pyrogallol autoxidation
rate, had highly scavenging capacity of hydroxyl free radical, and had dose dependent in scaveng
ing DPPH free radical scavenging. Their IC50 were 10, 0.49 mg/mL and 16.3 ug/mL respectively.
Futher research showed that water-soluble peptides from XNP had certain reducing power increased
as concentration increased, they also could inhibit the peroxidation of liposomes. ConclusionWa
ter-soluble peptides from XNP was a good anti-oxidant in vitro test. |
| Key words: Xylaria nigripes peptides anti-oxidation in vitro test |
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