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刘晓丹； 邓常清；.黄茂甲营和三七总皂营中主要有效成分
抗PC 12细胞氧化损伤的配伍研究[J].湖南中医药大学学报英文版,2012,32(1):8-12.[Click to copy
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| 黄茂甲营和三七总皂营中主要有效成分
抗PC 12细胞氧化损伤的配伍研究 |
| 刘晓丹；邓常清； |
| (湖南中医药大学分子病理实验室) |
| 摘要: |
| 目的探索黄茂甲葺和三七总皂葺中主要有效成分人参皂葺Rgl、人参皂葺Rbl、三七皂葺R1抗CoCh
诱导的PC12细胞氧化损伤的有效配伍剂量、方法采用CoCh诱导PC12细胞氧化损伤模型，以细胞乳酸脱氢酶
(LDH)漏出率为指标，研究4种有效成分对PC12细胞氧化损伤的有效机制浓度〔在此基础上按UH*(8s)均匀设计实
验，确定4种有效成分的有效配伍剂量〔结果4种有效成分都能抑制CoCI,诱导的PC12细胞LDH的漏出，与损伤组
相比差异有统计学意义(P<0.05 )、且随着药物浓度的增加，对LDH漏出率的抑制作用增强、黄茂甲葺和人参皂葺Rbl
在50 }e,mol儿，人参皂葺Rgl和三七皂葺R1在100 }e,mol儿浓度时LDH漏出抑制率约为80%} UH*(8s)均匀设计实验
多元逐步回归分析得:4种有效成分的8个不同浓度配伍都能抑制CoCI,诱导的PC12细胞LDH的释放，与损伤组相
比差异有统计学意义(P<0.05 )〔人参皂葺Rgl 50 }e,mol儿、黄茂甲葺0.781 25 }e,mol儿、人参皂葺Rbl和三七皂葺R1
各1.562 5 }e,mol/L配伍时抗PC12细胞氧化损伤的效应最强〔结论人参皂葺Rgl 50 }e,mol/L、黄茂甲葺0.781
25 }e,mol儿、人参皂葺Rbl和三七皂葺R1各1.562 5 }e,mol儿配伍组方为抗PC 12细胞氧化损伤的有效配伍剂量〔 |
| 关键词: 黄茂甲葺 人参皂葺R烈 人参皂葺Rbl 三七皂葺R1 配伍 PC12细胞 氧化损伤 均匀设计 |
| DOI： |
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| 基金项目:国家自然科学青年科学基金项日(81102557);教育部2010午度高等学校博卜学科点专项科研基金资助项目(20104323110001);湖
南省高校创新平台开放基金项目(11K050) |
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| Compatibility study of main effective components in PNSand AST on oxidative damage of PC12 |
| LIU Xiao-dan, DENG Chang-qing |
| (TCM University of Hunan) |
| Abstract: |
| (Abstract〕 Objective To investigate the effective compatibility dose of GinsenosideRgl, Gin-
senosideRbl, Astragaloside IV and NotoginsenosideRl against CoClz -induced oxidative injury on
PC12 cells. Methods The Lactate dehydrogenase (LDH) leakage ratio was calculated after LDH
levels in PC 12 cells induced by CoCL2 and cell culture medium were determined. The effec-
tive concentration of four active ingredients of anti一oxidative damage in PC12 cells were determined by LDH leakage rates and then the best dose compatibility of these four active
ingredients was identified according[()the uniform design of U8* (85). Results Compared
with model group，all the eight different concentrations of the four active ingredients could effec-
tively inhibit the release of LDH (P<0.05). Furthermore, the inhibiting rates of the LDH leakage
were increased with the increasing doses. The inhibition of LDH leakage rate in PC12 cells was 80% treated with 50 N.,mol/L Astragaloside}l and GinsenosideRbl, 100 N.,mol/L GinsenosideRgl and
NotoginsenosideRl. It showed that eight different concentrations of compatibility of four active in-
gradients could effectively inhibit the release of LDH and there were statistical significance be-
tween medicine groups and model group (P<0.05) analyzed by multiple regression analysis. There
was best effect of anti一(oxidative damage of PC12 cells induced by CoClz when treated with
50 N.,mol/L GinsenosideRgl, 0.78125 N.,mol/L Astragaloside It1，1.5625 N.,mol/L GinsenosideRbl and
NotoginsenosideRl. Conclusion The effective components compatibility doses of 50 N.,mol/L Gin-
senosideRgl, 0.78125 N.,mol/L Astragaloside It1，1.5625 N.,mol/L GinsenosideRbl and Notogin-
senosideRl are the best effective dose for anti-oxidative damage of PC12 cells induced by CoC12. |
| Key words: Astragaloside It1 GinsenosideRb 1 GinsenosideRgl NotoginsenosideRl effec-
tive compatibility PC 12 cells oxidative damage uniform design |
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