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万月一，蔡萍，张水寒*.土鳖虫超微粉体中尿U-I咤、次黄嘿吟的
含量测定[J].湖南中医药大学学报英文版,2011,31(9):36-37.[Click to copy
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| 土鳖虫超微粉体中尿U-I咤、次黄嘿吟的
含量测定 |
| 万月一，蔡萍，张水寒* |
| ((湖南省中医药研究院，湖南省中药粉体与创新药物省部共建重点实验室，
湖南省教育厅中药粉体技术创新团队，湖南长沙410013)) |
| 摘要: |
| 〔摘要〕目的建立HPLC同时测定上鳖虫超微粉体中核葺类成分尿崛陡、次黄嚓吟含量的方法〔方法色谱柱为
Hypersil C,H BDS柱(4.6 mmx250 mm,5 }e,m)，流动相0.05 mol/L磷酸氢二按水溶液，检测波长为254 nm，流速为
0.4 mL/min，柱温为25℃结果尿崛陡、次黄嚓吟分别在0.044-0.22 },g,0.042-0.21 },g范围内线性关系良好，平均
回收率分别为95.930Ic,97.320Ic, RSD分别为2.90Ic , 3.0%结论建立的方法简单、重复性好、准确可靠，可用于上鳖虫
中尿uw u,}、次黄n,}吟的含量测定、 |
| 关键词: 上鳖虫 超微粉体 含量测定 尿嘧啶；次黄嘌呤 |
| DOI： |
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| 基金项目:一国家公益性行业科研}1项(200807054) |
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| Determination of uraci and hypoxanthine contents in ultramicro power of Eupolyphaga |
| WAN Dan, CAI Ping, ZHANG Shui-han |
| (( Hunan Provincial
t7()n
and Ministry Cc)一〔constructive I}ey Laboratory of Altramicro Powder and Innova-
Drug, TCM Academy of Hunan, Changsha, Hunan 410013, China)) |
| Abstract: |
| (Abstract〕 Objective To develop a method for determination of
urac i
ultramicro
Hypersil
power of Eupolyphaga. Methods Chromatographic conditions
、、ere
and hypoxanthine in
as follows: column
BDS
C,8 (4.6
phosphate
mm X
250
[卜e
mm
5 N.,m), mobile
phase
、、as
0.05
mol/L. Two
ammom一
un
hydrogen
solution;
detective wavelength
、、as
254 nm, column temperature
、、as
250C and the flow rate
was 0.4 mL/min.
Results The
urac i
and hypoxanthine
、、ere
showed good
7，in
linearity
96.76%,
( r =0.999
with RSD
r=0.999 6)over the tested ranges. the
2.8% and 3.4%. Conclusion The method
average recoveries were 95.95%，
is simple, repeatable and accurate,
and can be used[()determine the nucleoside composition in ultramicro power of Eupolyphaga. |
| Key words: Eupolyphaga ultramicropower con[en[
determine uraci hypoxanthine |
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