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孙晓峰’.尤昭玲2.刘月一卓2.付灵梅2.龚力民3.吴霞.补肾活血方对PC 0小鼠未成熟卵母细胞
体外核成熟的影响[J].湖南中医药大学学报英文版,2011,31(3):27-30.[Click to copy
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| 补肾活血方对PC 0小鼠未成熟卵母细胞
体外核成熟的影响 |
| 孙晓峰’.尤昭玲2.刘月一卓2.付灵梅2.龚力民3.吴霞 |
| (湖南中医药大学基础医学院
;湖南中医药大学中西结合学院
;湖南中医药大学药学院) |
| 摘要: |
| 〔摘要〕目的探讨补肾活血方对PCO小鼠未成熟卵母细胞体外核成熟的影响、方法(1)制备补肾活血方含药
血清;}2)制作PCO小鼠模型;}3)将PCO小鼠生发泡(germinal vesicle , GV)期卵母细胞分别在不同采血时间获取的
补肾活血方含药血清培养液中进行体外培养，观察补肾活血方含药血清对生发泡破裂(germinal vesicle break-
down,GVBD)和第一极体(the first polar body,PBl)排出的时效关系结果(1)2 h和2.5 h药物血清组卵母细胞
GVBD的发生率高于正常血清组和对照组，培养后4 h差异有统计学意义(P<0.01) ; (2)2 h和2.5 h药物血清组卵
母细胞PB1的发生率高于正常血清组和对照组，培养后18 h差异有统计学意义(P<0.01)、结论2-2.5 h补肾活血
方含药血清对PCO小鼠未成熟卵母细胞体外核成熟具有明显促进作用、 |
| 关键词: 补肾活血方 血清 卵母细胞 生发泡破裂 第一极体 小鼠 紫石英 桑寄生 泽泻 |
| DOI： |
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| 基金项目:湖南省科技厅博卜后专项(2009RS3018);湖南省教育厅资助项日(2009C727) |
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| Effects of Bushen Huoxue decoctionnuclear maturation of immature PCOon in-vitrooocytes inmice |
| SUN Xiao-feng, YOU Zhao-ling, LIU Dan-zhuo, FU Ling-mei, GONG Li-min, WU Xia |
| (TCM University of Hunan) |
| Abstract: |
| (Abstract〕 Objective To investigate the effect of Bushen Huoxue decoction on in-vitro nu-
clear maturation of immature PCO oocytes in mice. Methods (1) The serum containing Bushen
Huoxue decoction was prepared. (2) The mice models with polycystic ovary were made. (3) The
immature oocytes isolated from the polycystic ovaries of the mice in the germinal vesicle (GV)
were cultured with the serum containing decoction collected from different time respectively and
the relation of GVBD and PB1 extrusion were detected. Results (1)GVBD after 2h and 2.5h
were higher in the serum containing decoction group than that in the controls and normal
serum groups, and the difference was the most notable after 4th hours (P<0.01). (2) The PB1 in
2 h and 2.5 h in the serum groups were higher than that in the controls and normal group, and
the difference was the most notable after 18th hours. Conclusion The serum containing Bushen
Huoxue decoction after 2一2.5 h is very effective[()promote in-vitro nuclear maturation of im-
mature ooc ytes significantly in PCO mice. |
| Key words: Bushen Huoxue decoction serum oocyte germinal vesicle breakdown PB1 mice fluoritum paraphyton rhizoma alismatis |
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