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谭元生，雍苏南，唐莹，张稳.中医不同治法对大鼠心肌缺血再灌注
损伤后细胞凋亡的影响[J].湖南中医药大学学报英文版,2011,31(1):18-21.[Click to copy
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| 中医不同治法对大鼠心肌缺血再灌注
损伤后细胞凋亡的影响 |
| 谭元生，雍苏南，唐莹，张稳 |
| (湖南中医药大学第一附属医院) |
| 摘要: |
| 〔摘要〕目的观察中医不同治法补气、活血、化痰对大鼠心肌缺血再灌注(( I/R)诱导细胞凋亡及凋亡相关基因
Bcl-2,Bax和氧自由基含量的影响、方法建立大鼠动脉粥样硬化在体心肌缺血再灌注模型，采用原位末端TUNEL
法检测心肌细胞凋亡指数(AI)，链酶亲和素一生物素一酶复合物(}BAB(7)免疫组化法检测凋亡相关基因Bcl-2,Bax蛋
白表达，常规方法检测丙二醇(MDA)含量、超氧化物歧化酶(80D)活性、结果与I/R组比较，中医补气、活血、化痰
法均能降低AI，有效清除氧自由基，差异有统计学意义(P<0.01);与I/R组比较，其中活血法和化痰法均能上调
Bcl-2表达、卜调Bax表达，差异有统计学意义(P<0.01)，最终达到保护I/R心肌的目的〔结论中医不同治法补
气、活血、化痰可能通过不同途径预防I/R心肌细胞发生凋亡.从而对I/R心肌具有保护作用、 |
| 关键词: 〔关键词〕心肌缺血再灌注损伤 补气法 活血法 化痰法 保元汤 血府逐疲汤 瓜萎燕白半夏汤 基因表达 氧自由基 大鼠 |
| DOI： |
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| 基金项目:湖南省教育厅科研项日(06C624) |
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| Effect of different treatments in TCM onmyocyte apoptosisin myocardial ischemia reperfusion in rats |
| TAN Yuan-sheng，YONG Su-nan，TANG Ying，Zhang Wen |
| (First Affiliated Hospital, TCM University of Hunan) |
| Abstract: |
| (Abstract〕 Objective To observe the effect of different treatments in TCM on myocyte apop
tosis and expression of Bcl-2 and Bax and the content of oxygen free radicals in myocardial is
chemia reperfusion in rats. Method The rats models in vivo of atherosclerosis myocardial ischemia
reperfusion were stablished, the apoptosis index (AI) was detected withTUNEL method in situ
end method, the apoptosis related genes Bcl-2Chain and Bax protein expression with avidin-bi
otin -enzyme complex (SABC) immunohistochemical method, and the activity of superox
ide dismutase (SOD) and the content of malondialdehyde (MDA) with routine method. Results
Compared with I/R group, all the treatments in TCM could reduce AI and remove effectively the
oxygen free radicals (P<0.01)，the activating blood and resolving phlegm methods could二、luce
the content of MDA and increase the activity of SOD and decrease Bax expression (P<0.01)，
and then, protect 1/R cardiac muscle. Conclusion The different treatments in TCM can restrain
the apoptosis of I/R cardiac muscle cells and[()protect consequently the I/R cardiac muscle. |
| Key words: ( Key words } apoptosis benef iting Qi therapy activating blood therapy resolving phlegm
therapy Baoyuan decoction Xuefu Zhuyu decoction Gualou Xiebai Banxia decoction gene expres-
sion oxygen free radicals rats |
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