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谢文彬, 谭若彤, 杨尚林, 谢玮琪, 任晋湘, 郭舟, 胡新颖, 李铁浪.基于p38 MAPK/PGC-1α信号通路探讨点按脾俞穴对慢性疲劳综合征大鼠骨骼肌重塑的影响[J].湖南中医药大学学报,2025,45(11):2106-2115[点击复制] |
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| 基于p38 MAPK/PGC-1α信号通路探讨点按脾俞穴对慢性疲劳综合征大鼠骨骼肌重塑的影响 |
| 谢文彬,谭若彤,杨尚林,谢玮琪,任晋湘,郭舟,胡新颖,李铁浪 |
| (湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察点按脾俞穴对慢性疲劳综合征（CFS）大鼠骨骼肌纤维类型及p38丝裂原活化蛋白激酶（p38 MAPK）/过氧化物酶体增殖物激活受体γ共激活因子1α(PGC-1α)信号表达的影响，探讨点按脾俞穴重塑CFS大鼠骨骼肌的作用机制。方法 将32只SD大鼠随机分为空白组8只、造模组24只。使用多因素复合刺激法建立CFS模型，造模周期为21 d。造模结束后，再将造模组大鼠随机分为模型组、点按脾俞组和人参皂苷组，每组8只。空白组、模型组予以捆绑固定和生理盐水灌胃1 mL/d；点按脾俞组予以捆绑固定、生理盐水灌胃1 mL/d和双侧脾俞穴点按；人参皂苷组大鼠给予捆绑固定及人参皂苷水溶液灌胃1 mL/d。均1次/d，共干预14 d。实验期间，进行实验大鼠一般情况半定量评分评估、体质量测量、力竭游泳实验、旷场实验；干预结束后，三磷酸腺苷（ATP）酶钙钴法染色检测大鼠竖脊肌中肌纤维类型，透射电镜观察竖脊肌超微结构，ELISA检测竖脊肌中ATP含量，实时荧光定量PCR法检测竖脊肌中PGC-1α mRNA表达，Western blot检测竖脊肌中p38 MAPK、PGC-1α蛋白表达水平。结果 干预结束后，与空白组比较，模型组大鼠一般情况半定量评分显著升高（P<0.01），体质量、力竭游泳时间、旷场实验运动总路程和进入中央区次数显著降低（P<0.01），竖脊肌肌原纤维排列紊乱，线粒体数量减少，Ⅰ型肌纤维比例减少，Ⅱ型肌纤维比例增加，ATP含量、PGC-1α mRNA表达、p38MAPK和PGC-1α蛋白相对表达量均显著降低（P<0.01）。与模型组比较，点按脾俞组和人参皂苷组大鼠一般情况半定量评分降低（P<0.05），体质量、力竭游泳时间、旷场实验运动总路程和进入中央区次数升高（P<0.05,P<0.01），竖脊肌肌原纤维排列更加整齐，线粒体数量增多，Ⅰ型肌纤维比例增加，Ⅱ型肌纤维比例减少，ATP含量、PGC-1α mRNA表达、p38 MAPK和PGC-1α蛋白相对表达量均升高（P<0.05,P<0.01）。与人参皂苷组比较，点按脾俞组大鼠一般情况半定量评分、体质量、力竭游泳时间、旷场实验运动总路程和进入中央区次数差异无统计学意义（P>0.05），竖脊肌电镜表现接近，Ⅰ型、Ⅱ型肌纤维比例接近，ATP含量、p38 MAPK和PGC-1α蛋白相对表达量差异无统计学意义（P>0.05）,PGC-1α mRNA表达显著升高（P<0.01）。结论 点按脾俞穴能有效改善CFS大鼠疲劳状态、运动能力以及焦虑状态，并促进骨骼肌肌纤维的重塑，其机制可能与激活p38 MAPK/PGC-1α信号通路有关。 |
| 关键词: 慢性疲劳综合征 点按法 脾俞穴 过氧化物酶体增殖物激活受体γ共激活因子1α 骨骼肌重塑 |
| DOI：10.3969/j.issn.1674-070X.2025.11.011 |
| 投稿时间：2025-07-05 |
| 基金项目:湖南省自然科学基金项目(2023JJ30454); 湖南中医药大学本科生科研创新基金项目(2023BKS090); 湖南省大学生创新训练项目(S202410541038)。 |
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| Exploring the effect of acupressure at Pishu（BL20） on skeletal muscle remodeling in rats with chronic fatigue syndrome based on the p38MAPK/PGC-1α signaling pathway |
| XIE Wenbin, TAN Ruotong, YANG Shanglin, XIE Weiqi, REN Jinxiang, GUO Zhou, HU Xinying, LI Tielang |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effects of acupressure at Pishu（BL20） on skeletal muscle fiber types and the expression of p38 mitogen-activated protein kinase（p38 MAPK）/peroxisome proliferator-activated receptor gamma coactivator 1 alpha（PGC-1α） signaling in rats with chronic fatigue syndrome（CFS）, and to explore the mechanism by which acupressure at Pishu（BL20）remodels skeletal muscle in CFS rats. Methods Thirty-two SD rats were randomly divided into a blank group（n=8） and a modeling group（n=24）. The CFS model was established in the modeling group using a multifactorial compound stimulation method for a modeling period of 21 days. After modeling, rats in the modeling group were further randomly divided into a model group, a Pishu（BL20） acupressure group, and a ginsenoside group, with 8 rats in each group. The blank group and model group received restraint fixation and intragastric administration of physiological saline at 1 m L/day. The Pishu（BL20） acupressure group received restraint fixation, intragastric administration of physiological saline at 1 mL/day, and bilateral acupressure at Pishu（BL20）. The ginsenoside group received restraint fixation and intragastric administration of ginsenoside aqueous solution at 1 m L/day. All interventions were performed once daily for 14 days. During the experiment, semiquantitative scoring of general condition, body weight measurement,exhaustive swimming test, and open field test were conducted. After the intervention, calcium-cobalt adenosine triphosphatase（ATPase） staining was used to detect muscle fiber types in the erector spinae of rats. Transmission electron microscopy was employed to observe the ultrastructure of the erector spinae. ELISA was used to measure the ATP contents in the erector spinae.Real-time quantitative polymerase chain reaction（PCR） was applied to detect the messenger ribonucleic acid（m RNA） expression of peroxisome proliferator-activated receptor gamma coactivator 1 alpha（PGC-1α） in the erector spinae. Western blot analysis was performed to assess the protein expression levels of p38 MAPK and PGC-1α in the erector spinae. Results Following the intervention, compared with the blank group, rats in the model group showed significantly higher semiquantitative scores of general condition（P＜0.01）, and significantly lower body weight, exhaustive swimming time, total distance traveled and number of entries into the central area in the open field test（P＜0.01）. In the model group, myofibrils in the erector spinae were disorganized, the number of mitochondria was reduced, the proportion of type I muscle fibers decreased while type II muscle fibers increased, and the ATP content, PGC-1α mRNA expression, as well as the relative expression of p38 MAPK and PGC-1α proteins were all significantly reduced（P＜0.01）. Compared with the model group, both the Pishu（BL20） acupressure group and the ginsenoside group showed decreased semiquantitative scores of general condition（P＜0.05）, and increased body weight, exhaustive swimming time, total distance traveled and number of entries into the central area（P＜0.05, P＜0.01）. In these two groups, the arrangement of myofibrils in the erector spinae became more orderly, the number of mitochondria increased, the proportion of type I muscle fibers increased while type II decreased, and ATP content, PGC-1α mRNA expression, and the relative expression of p38 MAPK and PGC-1α proteins were elevated（P＜0.05, P＜0.01）. Compared with the ginsenoside group, there was no statistically significant difference in semiquantitative score of general condition, body weight, exhaustive swimming time, total distance in the open field test, number of entries into the central area, ultrastructural observation under electron microscopy, proportions of type I and II muscle fibers, ATP content, and relative protein expression levels of p38 MAPK and PGC-1α between the Pishu（BL20） acupressure group and the ginsenoside group（P＞0.05）. However, the Pishu（BL20） acupressure group showed a significantly higher expression of PGC-1α m RNA（P＜0.01）.Conclusion Acupressure at Pishu（BL20） can effectively ameliorate fatigue, exercise capacity, and anxiety in rats with CFS, and promote skeletal muscle fiber remodeling. The underlying mechanism may be related to the activation of the p38 MAPK/PGC-1αsignaling pathway. |
| Key words: chronic fatigue syndrome acupressure Pishu（BL20） peroxisome proliferator-activated receptor gamma coactivator 1-alpha skeletal muscle remodeling |
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