| 引用本文: |
刘芮祺, 李雨晴, 肖阳, 刘萍, 丰正男, 傅音睿, 王志琪.去甲乌药碱配伍6-姜酚前后对衰竭心肌细胞影响的研究[J].湖南中医药大学学报,2025,45(4):647-653[点击复制] |
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| 去甲乌药碱配伍6-姜酚前后对衰竭心肌细胞影响的研究 |
| 刘芮祺,李雨晴,肖阳,刘萍,丰正男,傅音睿,王志琪 |
| (湖南中医药大学药学院, 湖南 长沙 410208;四川大学华西药学院, 四川 成都 610041) |
| 摘要: |
| 目的 比较去甲乌药碱配伍6-姜酚前后对衰竭心肌细胞的作用。方法 采用过氧化氢（H2O2）法复制衰竭H9c2细胞模型。将细胞分为空白组，模型组，6-姜酚组，5、10、20 μmol/L去甲乌药碱单用组以及分别与20 μmol/L 6-姜酚配伍组，共9组，观察细胞的线粒体形态，检测线粒体膜电位、Ca2+浓度、ATP含量及线粒体膜部分主动转运酶活性。结果 衰竭H9c2细胞复制条件为1 000 μmol/L H2O2干预2 h。与模型组相比，去甲乌药碱单用及与6-姜酚合用均明显改善模型细胞线粒体的微观结构损害。与单用去甲乌药碱比较，配伍6-姜酚对模型细胞具有协同保护作用，模型细胞的线粒体膜更为完整、边界清晰、嵴结构恢复，线粒体膜电位显著升高，细胞质及线粒体钙浓度均大幅降低，同时，ATP含量及Na+-K+-ATP酶、Ca2+-ATP酶、Ca2+-Mg2+-ATP酶活性均显著增强（P＜0.05，P＜0.01）。结论 去甲乌药碱与6-姜酚配伍可显著增强其对衰竭H9c2心肌细胞的保护作用，该作用可能与两者合用协同缓解线粒体钙超载、提高线粒体膜ATP酶活性、增加ATP的产量及促进线粒体功能恢复有关。 |
| 关键词: 去甲乌药碱 6-姜酚 H9c2心肌细胞 能量代谢 线粒体 |
| DOI：10.3969/j.issn.1674-070X.2025.04.009 |
| 投稿时间：2025-01-08 |
| 基金项目:湖南省教育厅重点项目（23A0285）；湖南省自然高校联合基金重点项目（2025JJ90002）；湖南省大学生创新创业项目（S202310541091）；湖南省自然科学基金项目（2021JJ80059）；长沙市自然科学基金项目（kq2014093）。 |
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| Effects of higenamine combined with 6-gingerol on failing cardiomyocytes before and after treatment |
| LIU Ruiqi, LI Yuqing, XIAO Yang, LIU Ping, FENG Zhengnan, FU Yinrui, WANG Zhiqi |
| (School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Huaxi School of Pharmacy, Sichuan University, Chengdu, Sichuan 610041, China) |
| Abstract: |
| Objective To compare the effects of higenamine before and after combination with 6-gingerol on failing cardiomyocytes. Methods A failing H9c2 cell model was established using hydrogen peroxide (H2O2). The cells were divided into nine groups: blank group, model group, 6-gingerol group, higenamine single-use groups (5, 10, 20 μmol/L), and higenamine combined with 6-gingerol (20 μmol/L) groups. The mitochondrial morphology of the cells was observed, and the mitochondrial membrane potential, Ca2+ concentration, ATP content, and mitochondrial membrane-associated active transport enzyme activity were measured. Results The replication conditions for inducing failing H9c2 cells were determined as 1000 μmol/L H2O2 for 2 hours. Compared with the model group, both higenamine monotherapy and its combination with 6-gingerol significantly alleviated mitochondrial ultrastructural damage. Compared with the use of higenamine alone, the combination with 6-gingerol had a synergistic protective effect on the model cells, as evidenced by a more intact mitochondrial membrane, clearer boundaries, and restored cristae structure. Additionally, mitochondrial membrane potential was significantly higher, cytoplasmic and mitochondrial Ca2+ concentrations were markedly lower, and ATP content, along with the activities of Na+-K+-ATPase, Ca2+-ATPase, and Ca2+-Mg2+-ATPase, significantly enhanced (P＜0.05, P＜0.01). Conclusion The combination of higenamine and 6-gingerol can significantly enhance the protective effect on failing H9c2 cardiomyocytes. This effect may be related to their synergistic action in alleviating mitochondrial calcium overload, increasing mitochondrial ATPase activity, promoting ATP production, and restoring mitochondrial function. |
| Key words: higenamine 6-gingerol H9c2 cardiomyocytes energy metabolism mitochondria |
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