| 引用本文: |
汤小康, 肖可, 王攀, 邱礼国, 周长征, 唐成剑.寒湿阻滞型腰痛大鼠模型构建及独活寄生汤干预效果的实验研究[J].湖南中医药大学学报,2024,44(6):976-981[点击复制] |
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| 寒湿阻滞型腰痛大鼠模型构建及独活寄生汤干预效果的实验研究 |
| 汤小康,肖可,王攀,邱礼国,周长征,唐成剑 |
| (湖南中医药大学第一附属医院, 湖南 长沙 410007) |
| 摘要: |
| 目的 构建大鼠寒湿阻滞型腰痛动物模型，评估独活寄生汤对该模型的干预效果。方法 24只雄性SD大鼠适应性喂养7 d后，随机分为空白组、模型组和独活寄生汤组。空白组仅腹腔注射戊巴比妥钠麻醉，模型组、独活寄生汤组在腹腔注射戊巴比妥钠麻醉下行“腰椎后柱结构部分切除术”，并在寒湿环境下饲养6周。造模2周后，空白组、模型组予等体积蒸馏水灌胃，独活寄生汤组予独活寄生汤18.6 g/（kg·d）灌胃，均连续灌胃4周。比较各组大鼠一般情况、自发性疼痛行为学评分和神经功能评分，CT三维成像评估脊柱生理曲度形态改变情况，椎间盘组织病理切片评估椎间盘髓核病变程度。结果 造模后1周，与空白组比较，模型组、独活寄生汤组开始出现少动、皮毛无光泽、嗜睡蜷缩、反应迟钝、大便稀溏表现，体质量减轻（P<0.05），自发性疼痛行为学评分和神经功能评分升高（P<0.05）；给药后3周，与模型组比较，独活寄生汤组疼痛行为学评分和神经功能评分降低（P<0.05）。给药后4周，与模型组比较，独活寄生汤组大鼠脊柱CT三维成像形态退变不明显，椎间盘组织病理切片显示，独活寄生汤组椎间盘髓核病变不明显。结论 “腰椎后柱结构部分切除术+寒湿环境饲养”可成功构建SD大鼠寒湿阻滞型腰痛动物模型，独活寄生汤对该模型有较好的干预效果。 |
| 关键词: 腰痛 寒湿阻滞 独活寄生汤 动物模型 腰椎间盘退变 |
| DOI：10.3969/j.issn.1674-070X.2024.06.007 |
| 投稿时间：2023-12-11 |
| 基金项目:湖南省自然科学基金青年基金项目（2022JJ40333）；湖南省卫生健康委员会科研计划项目（202104071103）；湖南省中医药科研计划项目（2021251）；湖南中医药大学校级科研基金项目（ZYYDX201728）。 |
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| Experimental study on construction of rat model of lower back pain due to cold dampness stagnation and intervention effects of Duhuo Jisheng Decoction |
| TANG Xiaokang, XIAO Ke, WANG Pan, QIU Liguo, ZHOU Changzheng, TANG Chengjian |
| (The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To construct the rat model of lower back pain due to cold dampness stagnation, and to evaluate the intervention effects of Duhuo Jisheng Decoction (DHJSD) on this model. Methods After 7 d of adaptive feeding, 24 male SD rats were randomized into blank group, model group, and DHJSD group. The blank group was only anesthetized by intraperitoneal injection of pentobarbital sodium, while the model group and DHJSD group were anesthetized by intraperitoneal injection of pentobarbital sodium for "partial resection of posterior column structure of the lumbar spine" and were fed in a cold and humid environment for six weeks. After two weeks of modeling, the blank group and model group were given an equal volume of distilled water by gavage, while the DHJSD group was administered DHJSD 18.6 g/(kg·d) by gavage for four consecutive weeks. The general condition, spontaneous pain behavior score, and neurological function score of rats in each group were compared. CT three-dimensional imaging was used to evaluate the morphological changes of the spinal physiological curvature, and the pathological section of intervertebral disc tissue was used to evaluate the severity of intervertebral disc nucleus pulposus lesions. Results After one week of modeling, compared with the blank group, the model group and the DHJSD group began to exhibit less movement, dull fur, drowsiness and curling up, slow response, loose stool, weight loss (P<0.05), and increased spontaneous pain behavior score and neurological function score (P<0.05). After three weeks of administration, compared with the model group, the pain behavior score and neurological function score of the DHJSD group decreased (P<0.05). After four weeks of administration, compared with the model group, the spinal CT three-dimensional imaging showed no significant morphological degeneration in the DHJSD group, and the pathological section of intervertebral disc tissue also showed no obvious intervertebral disc nucleus pulposus lesions in the DHJSD group. Conclusion The method of "partial resection of posterior column structure of the lumbar spine+feeding in a cold and humid environment" can successfully construct the SD rat model of low back pain due to cold dampness stagnation, and DHJSD has good intervention effects on this model. |
| Key words: lower back pain cold dampness stagnation Duhuo Jisheng Decoction animal model intervertebral disc degeneration |
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