| 引用本文: |
刘恃君, 陆玉爱, 侯师艺, 戈玉莹, 夏伯候, 谢明霞, 李亚梅.补血益母丸对气血双虚小鼠的毛发代谢组学研究[J].湖南中医药大学学报,2024,44(6):982-990[点击复制] |
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| 补血益母丸对气血双虚小鼠的毛发代谢组学研究 |
| 刘恃君,陆玉爱,侯师艺,戈玉莹,夏伯候,谢明霞,李亚梅 |
| (湖南中医药大学药学院/湘产大宗药材品质评价湖南省重点实验室, 湖南 长沙 410208) |
| 摘要: |
| 目的 探讨补血益母丸对气血双虚小鼠的毛发代谢组学研究。方法 采用腹腔注射环磷酰胺（cyclophosphamide，CTX）及眼眶放血，建立气血双虚小鼠模型后，将动物分为模型组、补血益母丸低剂量组和补血益母丸高剂量组，另设正常组，并连续10 d对小鼠进行灌胃补血益母丸1.82、7.28 g·kg-1干预。通过气相色谱飞行时间质谱（gas chromatography time-of-flight mass spectrometry,GC-TOF-MS）技术对各实验组小鼠的毛发进行分析，采用主成分分析（principal component analysis, PCA）和正交偏最小二乘法判别分析（orthogonal partial least-squares discriminant analysis, OPLS-DA）寻找并发现补血益母丸作用于气血双虚模型小鼠的差异代谢物。结果 毛发中有5个差异性代谢物与CTX致免疫低下型气血双虚证相关，主要涉及酪氨酸代谢（tyrosine metabolism）和初级胆汁酸生物合成（primary bile acid biosynthesis）；经补血益母丸治疗后代谢轮廓趋近正常组，有11个代谢物涉及3条代谢通路，推测补血益母丸对气血双虚证的治疗作用可能通过调控氨基酸代谢有关。结论 本研究从毛发代谢组学角度为中医气血双虚病症及补血益母丸的干预提供了氨基酸代谢、胆汁酸代谢机制层面的解释，部分揭示了补血益母丸益气补血作用机制，为其临床运用提供了一定理论依据。 |
| 关键词: 代谢组学 补血益母丸 气血双虚 气质联用 毛发代谢 |
| DOI：10.3969/j.issn.1674-070X.2024.06.008 |
| 投稿时间：2023-11-09 |
| 基金项目:湖南省自然科学基金项目（2022JJ50008）；湖南省卫生健康委员会2023年度科研计划课题（B202313057639）；湖南省教育厅科学研究项目（23A0307，23B0383）；湖南中医药大学重点学科中药学科（校行发规字〔2023〕2号）；2023年湖南中医药大学研究生创新项目（2023CX153）。 |
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| Hair metabolomics study on Buxue Yimu Pill treating mice with qi-blood deficiency |
| LIU Shijun, LU Yu'ai, HOU Shiyi, GE Yuying, XIA Bohou, XIE Mingxia, LI Yamei |
| (College of Pharmacy/Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province, Hunan University of Chinese Medicine, Changsha 410208, China) |
| Abstract: |
| Objective To study Buxue Yimu Pill (BXYMP) treating mice with qi-blood deficiency based on hair metabolomics. Methods A mouse model of qi-blood deficiency was established by intraperitoneal injection of cyclophosphamide (CTX) and orbital bloodletting. After modeling, the animals were divided into model group, as well as low- and high-dose BXYMP groups which were intragastrically treated with BXYMP at doses of 1.82 and 7.28 g·kg-1 respectively for 10 consecutive days. Additionally, a normal group was set up for comparison. Gas chromatography time-of-flight mass spectrometry (GC-TOF-MS) was used to analyze the hair of mice from each experimental group. Principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) were employed to discover and identify the differential metabolites in the mice with qi-blood deficiency after the treatment of BXYMP. Results Five differential metabolites in the hair were associated with CTX-induced immunodeficiency of qi-blood deficiency pattern, mainly involving tyrosine metabolism and primary bile acid biosynthesis; after treatment with BXYMP, the metabolic profile approached that of the normal group, with 11 metabolites involved in three metabolic pathways. It was speculated that the therapeutic effect of BXYMP on qi-blood deficiency pattern may be related to the regulation of amino acid metabolism. Conclusion This study explains the TCM pattern of qi-blood deficiency and the intervention of BXYMP on it in ways of the mechanisms of amino acid metabolism and bile acid metabolism from the perspective of hair metabolomics. It partially reveals the mechanism of action of BXYMP in supplementing qi and tonifying blood, providing a certain theoretical basis for its clinical application. |
| Key words: metabolomics Buxue Yimu Pill qi-blood deficiency gas chromatography time-of-flight mass spectrometry hair metabolism |
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