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喻雄华,辜转荣,程铁兵,向栋,喻轶.萸芪糖浆调节Th1/Th2平衡改善免疫性重症肌无力大鼠免疫功能的研究[J].湖南中医药大学学报,2023,43(6):961-967[点击复制] |
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| 萸芪糖浆调节Th1/Th2平衡改善免疫性重症肌无力大鼠免疫功能的研究 |
| 喻雄华,辜转荣,程铁兵,向栋,喻轶 |
| (仙桃市中医医院, 湖北 仙桃 433000;湖北中医药大学检验学院, 湖北 武汉 430065) |
| 摘要: |
| 目的 观察萸芪糖浆对重症肌无力大鼠Th1/Th2平衡的调节及改善重症肌无力大鼠免疫应答的影响。方法 取70只大鼠采用乙酰胆碱受体前体蛋白（acetylcholine receptor precursor protein, AchR）多点注射造模，筛选50只自身免疫性重症肌无力大鼠模型，随机分为模型组、泼尼松组、萸芪糖浆低剂量组、萸芪糖浆中剂量组、萸芪糖浆高剂量组，每组10只，另外设置空白组10只。造模完成后，分别给予萸芪糖浆31.84、15.92、7.96 g·（kg·d）-1灌胃，泼尼松组给予泼尼松5.4 g·（kg·d）-1灌胃，空白组、模型组给予同体积生理盐水灌胃。30 d后取材，比较各组大鼠体质量、胸腺指数、脾脏指数；疲劳试验及Lennon 14评分评估各组大鼠肌无力症状；ELISA法检测血清AchR、肿瘤坏死因子-α（tumor necrosis factor-α, TNF-α）及白细胞介素-10（interleukin-10, IL-10）炎性细胞因子水平；流式细胞仪检测T细胞中CD4+/CD8+比值。结果 与空白组比较，模型组Lennon 14评分，血清AchR、TNF-α均升高（P<0.01），体质量、脾脏指数、胸腺指数、IL-10及T细胞中CD4+/CD8+比例均降低（P<0.01）；与模型组比较，各组的脾脏指数、胸腺指数均显著升高（P<0.01），萸芪糖浆中剂量组和萸芪糖浆高剂量组Lennon 14评分，血清AchR、TNF-α均降低（P<0.01），体质量、IL-10及T细胞中CD4+/CD8+比例均升高（P<0.01）；与泼尼松组比较，萸芪糖浆低剂量组体质量、脾脏指数、胸腺指数显著降低（P<0.05，P<0.01），萸芪糖浆中剂量组Lennon 14评分显著下降（P<0.05），萸芪糖浆中、高剂量组TNF-α降低（P<0.01），脾脏指数、胸腺指数、IL-10、T细胞中CD4+/CD8+比值均升高（P<0.01）；与萸芪糖浆低剂量组比较，萸芪糖浆中、高剂量组脾脏指数、胸腺指数、IL-10、T细胞中CD4+/CD8+比值显著升高（P<0.05，P<0.01），Lennon 14评分、TNF-α降低（P<0.05，P<0.01）。结论 萸芪糖浆能有效治疗重症肌无力大鼠肌无力症状，其机制可能与维持Th1/Th2平衡相关。 |
| 关键词: 重症肌无力|Th1/Th2平衡|萸芪糖浆|炎症因子|大鼠 |
| DOI：10.3969/j.issn.1674－070X.２０23.06.001 |
| 投稿时间：2023-01-14 |
| 基金项目:湖北省自然科学基金项目（2021CFB234）；湖北省中医药重点科研项目（ZY2021Z016）；湖北省教育厅2021年度科研计划项目（Q20212002）。 |
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| Regulation of Yuqi Syrup on Th1/Th2 balance to improve the immune function in rats with immune myasthenia gravis |
| YU Xionghua,GU Zhuanrong,CHENG Tiebing,XIANG Dong,YU Yi |
| (Xiantao Hospital of Traditional Chinese Medicine, Xiantao, Hubei 433000, China;School of Laborabory Medicine, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, China) |
| Abstract: |
| Objective To observe Yuqi Syrup's regulation on Th1/Th2 balance and and its effects on the improvement of the immune response in myasthenia gravis rats. Methods A total of 70 rats were modeled by multi-point injections of acetylcholine receptor precursor protein (AchR) and 50 of them (modeled rats with autoimmune myasthenia gravis) were screened and randomly divided into model group, prednisone group, low-dose, medium-dose and high-dose Yuqi Syrup groups, with 10 rats in each group. And another 10 rats were set up as the blank group. After modeling, the rats were given Yuqi Syrup at a dose of 31.84, 15.92, 7.96 g·(kg·d)-1 by gavage respectively. The prednisone group were given prednisone at a dose of 5.4 g·(kg·d)-1 by gavage, while blank group and model group were given the same volume of saline by gavage. After 30 d, the rats' body mass, thymus index, and spleen index of each group were compared, and the fatigue testing and Lennon 14 score were adopted to evaluate the rats' myasthenic symptoms. The levels of serum AchR, tumor necrosis factor-α (TNF-α), and interleukin-10 (IL-10) inflammatory cytokines were determined by ELISA. The flow cytometry was used to examine the CD4+/CD8+ ratio in T cells. Results Compared with blank group, the Lennon 14 score, serum AchR, and TNF-α levels in model group increased (P<0.01), while the body mass, spleen index, thymus index, IL-10, and CD4+/CD8+ ratio of T cells decreased (P<0.01). Compared with model group, the spleen index and thymus index increased significantly in all groups (P<0.01), and the Lennon 14 score, the serum AchR and TNF-α decreased in medium- and high-dose Yuqi Syrup groups (P<0.01), while the body mass, IL-10 and CD4+/CD8+ ratio in T cells increased (P<0.01). Compared with prednisone group, the body mass, spleen index and thymus index decreased significantly in low-dose Yuqi Syrup group (P<0.05, P<0.01), the Lennon 14 score decreased significantly in medium-dose Yuqi Syrup group (P<0.05), and TNF-α decreased in medium- and high-dose Yuqi Syrup groups (P<0.01), while the spleen index, thymus index, IL-10, and CD4+/CD8+ ratio of T cells increased (P<0.01). Compared with low-dose Yuqi Syrup group, the spleen index, thymus index, IL-10, and CD4+/CD8+ ratio of T cells had increased significantly (P<0.05, P<0.01), while the Lennon 14 score and TNF-α decreased (P<0.05, P<0.01) in medium- and high-dose Yuqi Syrup groups. Conclusion Yuqi Syrup can effectively treat the myasthenic symptoms in rats, and its mechanism may be related to maintaining the Th1/Th2 balance. |
| Key words: myasthenia gravis|Th1/Th2 balance|Yuqi Syrup|inflammatory factors|rats |
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