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王建珠,鲍云帆,杨小存,田志刚,唐燕定,郝锋.按揉承山穴对CCI模型大鼠机械足反射阈值及坐骨神经形态学影响研究[J].湖南中医药大学学报,2023,43(1):106-110[点击复制] |
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| 按揉承山穴对CCI模型大鼠机械足反射阈值及坐骨神经形态学影响研究 |
| 王建珠,鲍云帆,杨小存,田志刚,唐燕定,郝锋 |
| (南京中医药大学针灸推拿学院, 江苏 南京 210023;南京医科大学附属儿童医院, 江苏 南京 210093) |
| 摘要: |
| 目的 观察按揉承山穴对坐骨神经慢性压迫损伤（chronic constriction injury of the sciatic nerve,CCI）模型大鼠的机械足反射阈值（paw withdrawal threshold,PWT）及坐骨神经形态学的影响。方法 将24只雄性SD大鼠随机分为空白组（N组）、模型组（M组）和按揉承山穴组（T组）。T组大鼠于CCI造模术后4d开始在其右后肢承山穴进行推拿按揉法操作，10min/次，1次/d，连续干预14d。观察造模前及造模后1d、3d、7d、10d、14d、17d的PWT，实验结束后对各组实验动物取材，将大鼠坐骨神经行HE染色后观察坐骨神经形态学变化。结果 与N组相比，M组大鼠的PWT在造模后1d出现下降（P<0.05），3d、7d、10d、14d、17d持续降低（P<0.01）并逐渐保持稳定，说明CCI模型大鼠在造模术后出现机械痛觉过敏。与造模前相比，T组大鼠的PWT在造模后1d出现明显下降，在3d达到最低（P<0.01），说明CCI模型大鼠造模成功。从第4天起行按揉承山穴干预，至14d，PWT与造模前相比差异有统计学意义（7d，P<0.01；10d、14d，P<0.05）；至17d与造模前差异无统计学意义（P>0.05）。与N组相比，T组大鼠的PWT在CCI造模术后有明显降低（1d、3d、7d，P<0.01；14d，P<0.05），10d及17d的PWT与N组差异无统计学意义（P>0.05）。HE染色发现，T组大鼠的坐骨神经外膜及神经束膜较N组有所增厚，并在周围可见结缔组织包裹且血供丰富，可见髓鞘完整的正常形态神经纤维。结论 按揉承山穴对CCI模型大鼠的PWT有改善作用，其镇痛效应明显；按揉承山穴对坐骨神经的形态学产生了一定的改变，可能与手法的镇痛机制相关。 |
| 关键词: 坐骨神经慢性压迫损伤 按揉法 承山穴 机械足反射阈值 坐骨神经 镇痛 |
| DOI：10.3969/j.issn.1674-070X.2023.01.016 |
| 投稿时间：2022-06-18 |
| 基金项目:江苏省高等学校自然科学研究面上项目（19KJB360013）。 |
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| Effects of pressing and kneading "Chengshan" (BL57) on the paw withdrawal threshold and the sciatic morphology of CCI model rats |
| WANG Jianzhu,BAO Yunfan,YANG Xiaocun,TIAN Zhigang,TANG Yanding,HAO Feng |
| (College of Acupuncture and Tuina, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China;Children's Hospital of Nanjing Medical University, Nanjing, Jiangsu 210093, China) |
| Abstract: |
| Objective To observe the effects of pressing and kneading "Chengshan" (BL57) on the paw withdrawal threshold (PWT) and the sciatic morphology of chronic constriction injury (CCI) of the sciatic nerve model rats. Methods A total of 24 male SD rats were randomly divided into blank group (group N), CCI model group (group M), and CCI model with pressing and kneading "Chengshan" (BL57) group (group T). Group T was treated with pressing and kneading manipulation on "Chengshan" (BL57) from the 4th day after molding, once daily for 10 min, 14d totally. The PWT before modeling, and on the 1st, 3rd, 7th, 10th, 14th and 17th days after modeling were all observed. In addition, samples were collected from each group. The morphologic changes of sciatic nerve were then observed after HE staining. Results Compared with group N, group M showed significant decrease in PWT (on the 1st day after modeling, P<0.05; on the 3rd, 7th, 10th, 14th and 17th days after modeling, P<0.01). The results showed that CCI model rats developed mechanical hyperalgesia after modeling. Compared with that before modeling, the PWT values of group T decreased significantly on the 1st day after modeling, and reached the lowest on the 3rd day after modeling (P<0.01). It identified the success of CCI modeling. Groups before and after modelling showed significant difference in PWT due to pressing and kneading "Chengshan" (BL57) from the 4th day to the 14th day after modeling (on the 7th day after modeling, P<0.01; on the 10th and 14th days after modeling, P<0.05); no significant difference was shown on the 17th day (P>0.05). Compared with group N, the PWT of rats in group T decreased significantly after modeling (on the 1st, 3rd and 7th days after modeling, P<0.01; on the 14th day after modeling, P<0.05); compared with group N, there are no significant differences in PWT on the 10th and 17th day (P>0.05). HE staining showed that the sciatic nerve adventitia and nerve fascicular membrane of rats in Group T were thicker than those in the group N, the connective tissues were wrapped around and blood supply was abundant, and the normal shape nerve fibers were shown. Conclusion Pressing and kneading manipulations on "Chengshan" (BL57) can improve the PWT of CCI model rats, and it has the apparent analgesic effects. Moreover, pressing and kneading "Chengshan" (BL57) has changed the morphology of sciatic nerve, which may be related to the analgesic mechanism of the manipulation. |
| Key words: chronic constriction injury of the sciatic nerve pressing and kneading manipulation "Chengshan" (BL57) paw withdrawal threshold sciatic nerve analgesia |
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