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周竞颖,来奕恬,丁攀婷,张国山,刘密.针灸对克罗恩病模型大鼠天枢穴局部皮肤中缓激肽、CGRP和NPY的影响[J].湖南中医药大学学报,2023,43(1):100-105[点击复制] |
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| 针灸对克罗恩病模型大鼠天枢穴局部皮肤中缓激肽、CGRP和NPY的影响 |
| 周竞颖,来奕恬,丁攀婷,张国山,刘密 |
| (湖南中医药大学研究生院, 湖南 长沙 410208;湖南中医药大学针灸推拿与康复学院, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察艾灸与针刺对克罗恩病模型大鼠天枢穴局部皮肤中缓激肽（bradykinin,BK）、降钙素基因相关肽（calcitonin gene related peptid,CGRP）、神经肽Y（neuropeptide Y,NPY）含量的影响。方法 将50只SD大鼠随机分为正常组、模型组、艾灸组、针刺组和西药组，每组10只。除正常组外，其余各组应用2,4,6-三硝基苯磺酸（2,4,6-trinitrobenzenesulfonic acid,TNBS）灌肠的方法制备克罗恩病肠纤维化大鼠模型。模型制备成功后，分别采用艾灸和针刺的方法干预艾灸组和针刺组大鼠双侧天枢穴和上巨虚穴，每次15min；西药组大鼠给予美沙拉嗪肠溶片溶液灌胃。上述干预均1次/d，连续干预7d。干预结束后，肉眼和HE染色观察各组大鼠结肠病理形态学变化；剪取各组大鼠天枢穴局部皮肤，ELISA法检测BK的蛋白表达，Western blot法检测CGRP、NPY的蛋白表达。结果 与正常组相比，模型组大鼠肠壁可见典型鹅卵石样改变、黏膜充血水肿等病理形态学损伤，天枢穴局部皮肤中BK含量显著升高（P<0.01），CGRP相对表达量显著降低（P<0.05），NPY相对表达量差异无统计学意义（P>0.05）。与模型组相比，各治疗组大鼠肠黏膜受损情况明显较轻，天枢穴局部皮肤中BK含量均显著降低（P<0.01），CGRP相对表达量均显著升高（P<0.01或P<0.05），NPY相对表达量差异无统计学意义（P>0.05）。针刺组、艾灸组和西药组3组之间相比，天枢穴局部皮肤中BK、CGRP和NPY的差异均无统计学意义（P>0.05）。结论 克罗恩病大鼠天枢穴局部皮肤中BK、CGRP的变化可能是肠腑病变在体表的客观反映，艾灸、针刺对克罗恩病大鼠天枢穴局部皮肤中BK、CGRP具有调节作用。 |
| 关键词: 克罗恩病 针灸 缓激肽 降钙素基因相关肽 神经肽Y 天枢 上巨虚 |
| DOI：10.3969/j.issn.1674-070X.2023.01.015 |
| 投稿时间：2022-03-17 |
| 基金项目:国家自然科学基金项目（81603705，81774438）；中国博士后科学基金项目（2107M612567）；湖南省自然科学基金项目（2021JJ30513，2017JJ3245）；湖南省教育厅科学研究项目（20B444）。 |
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| Effects of acupuncture and moxibustion on bradykinin, CGRP and NPY in the local skin of "Tianshu" (ST25) in Crohn's disease model rats |
| ZHOU Jingying,LAI Yitian,DING Panting,ZHANG Guoshan,LIU Mi |
| (Graduate School of Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;College of Acupuncture & Tuina and Rehabilitation of Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effects of acupuncture and moxibustion on the content of bradykinin (BK), calcitonin gene-related peptide (CGRP) and neuropeptide Y (NPY) in the local skin of "Tianshu" (ST25) in Crohn's disease model rats. Methods Fifty SD rats were randomly divided into normal group, model group, moxibustion group, acupuncture group and western medicine group, with 10 rats in each group. Except for the normal group, the rats in the other groups were treated with 2,4,6-trinitrobenzenesulfonic acid (TNBS) enema to prepare intestinal fibrosis rat model of Crohn's disease. Moxibustion and acupuncture were respectively used to intervene the bilateral "Tianshu" (ST25) and "Shangjuxu" (ST37) of the moxibustion group and acupuncture group, for 15 min each time. Meanwhile, the western medicine group was given mesalazine enteric-coated tablet solution by gavage. The above interventions were performed once a day for 7 consecutive days. After the intervention, the pathological changes of colon tissue in rats of each group were observed by the naked eye and HE staining. The local skin of "Tianshu" (ST25) in rats of each group was cut, then the protein expression of bradykinin was detected by ELISA method, and the protein expression levels of CGRP and NPY were detected by Western blot. Results Compared with the normal group, the model group showed pathological damages of the intestinal wall, such as typical cobblestone-like changes, mucosal congestion and edema. Moreover, for the local skin of "Tianshu" (ST25) in the model group, the content of BK (P<0.01) was significantly higher, the relative expression of CGRP (P<0.05) was significantly lower, and the relative expression of NPY was not significant different (P>0.05). Compared with the model group, in each treatment group, the intestinal mucosal damage was apparently lighter, the content of BK was significantly reduced (P<0.01), the relative expression of CGRP significantly increased (P<0.01 or P<0.05), and there was no statistical difference (P>0.05) in the relative expression of NPY in the local skin of "Tianshu" (ST25). There was no statistical difference (P>0.05) in the content of BK, CGRP and NPY in the local skin of "Tianshu" (ST25) among the acupuncture group, moxibustion group and western medicine group. Conclusion The changes of BK and CGRP in the local skin of "Tianshu" (ST25) in Crohn's disease rats may be the objective manifestation of intestinal lesions. Acupuncture and moxibustion may regulate BK and CGRP in the local skin of "Tianshu" (ST25) in rats with Crohn's disease. |
| Key words: Crohn's disease acupuncture and moxibustion bradykinin calcitonin gene-related peptide neuropeptide Y "Tianshu" (ST25) "Shangjuxu" (ST37) |
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