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莫晓玮,唐凯锐,王静.黄芩汤对溃疡性结肠炎湿热证小鼠肠道菌群的影响[J].湖南中医药大学学报,2022,42(6):917-922[点击复制] |
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| 黄芩汤对溃疡性结肠炎湿热证小鼠肠道菌群的影响 |
| 莫晓玮,唐凯锐,王静 |
| (暨南大学中医学院, 广东 广州 510632) |
| 摘要: |
| 目的 探讨黄芩汤对溃疡性湿热证小鼠肠道菌群的影响。方法 将C57BL/6雄性小鼠随机分为空白对照（NC）组、模型（DSS）组、黄芩汤（HQD）组，每组7只。除NC组正常饲养外，其余2组均给予高脂高糖饮食，同时暴露在温度32℃、湿度90%环境中，每天持续16 h，连续15 d后，自由饮用2.5%葡聚糖硫酸钠水7 d。HQD组给予黄芩汤（9.1 mg/kg），NC组、DSS组均给予同体积蒸馏水灌胃7 d，期间观察小鼠一般情况，评价其疾病活动指数（disease activity index,DAI）。处死小鼠后，取中段结肠评价小鼠病理改变，取盲肠内容物进行16S rDNA高通量测序。结果 与NC组比较，DSS组小鼠DAI评分显著增高，结肠黏膜结构受损，肠道菌群多样性降低，变形菌（Proteobacteria）、拟杆菌属（Bacteroides）、大肠埃希菌-志贺菌属（Escherichia-Shigella）、副拟杆菌属（Parabacteroides）、瘤胃球菌属（Ruminococcus torques）相对丰度显著提高，厚壁菌门（Firmicutes）、布劳特氏菌属(Blautia)、毛螺菌属(Lachnospiraceae NK4A136 group)相对丰度显著下降；经黄芩汤治疗后，能显著减轻模型小鼠的疾病活动，修复其结肠的病理损伤，逆转肠道菌群的紊乱。结论 黄芩汤可能通过提高菌群多样性及调节溃疡性结肠炎湿热证小鼠菌群丰度，提升肠黏膜的完整性以发挥生物屏障功能，从而发挥治疗作用。 |
| 关键词: 溃疡性结肠炎 湿热证 黄芩汤 肠道菌群 菌群多样性 菌群相对丰度 |
| DOI：10.3969/j.issn.1674－070X.２０22.06.008 |
| 投稿时间：2021-12-16 |
| 基金项目:国家自然科学基金项目(81703957);广东省中医药局科研项目(20181069)。 |
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| Effect of Huangqin Decoction on intestinal flora in mice with damp-heat syndrome of ulcerative colitis |
| MO Xiaowei,TANG Kairui,WANG Jing |
| (College of Traditional Chinese Medicine, Ji'nan University, Guangzhou, Guangdong 510632, China) |
| Abstract: |
| Objective To explore the effect of Huangqin Decoction on the intestinal flora of mice with damp-heat syndrome of ulcerative colitis. Methods C57BL/6 male mice were randomly divided into blank control (NC) group, model (DSS) group and Huangqin Decoction (HQD) group, with seven mice in each group. Except for the normal feeding in the NC group, the other two groups were given a high-fat and high-sugar diet, and were exposed to a temperature of 32℃ and a humidity of 90% for 16 h a day. After 15 consecutive days, they were given free access to 2.5% dextran sulfate sodium water for 7 d. The HQD group was given Huangqin Decoction (9.1 mg/kg), NC group and the DSS group were given the same volume of distilled water for 7 d. During the period, the general condition of the mice was observed and their disease activity index (DAI) was evaluated. After the mice were sacrificed, the middle colon was taken to evaluate the pathological changes of the mice, and the content of the cecum was taken for 16S rDNA high-throughput sequencing. Results Compared with the NC group, the DAI score of the mice in DSS group was significantly increased, the colonic mucosal structure was damaged, and the diversity of intestinal flora was decreased, the relative abundances of Proteobacteria, Bacteroides, Escherichia-Shigella, Parabacteroides, and Ruminococcus torques were significantly increased, and the relative abundances of Firmicutes, Blautia, and Lachnospiraceae NK4A136 group were significantly decreased. The treatment of Huangqin Decoction can significantly reduce the disease activity of mice after modeling, repair the pathological damage of its colon and reverse the disturbance of intestinal flora. Conclusion Huangqin Decoction may play a therapeutic role by increasing the diversity of the flora and regulating the abundance of the flora in mice with damp-heat syndrome of ulcerative colitis, and improving the integrity of the intestinal mucosa to exert the function of biological barrier. |
| Key words: ulcerative colitis damp-heat Huangqin Decoction intestinal flora flora diversity relative abundance of flora |
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