| 引用本文: |
王语晴,郭婉琴,刘欣欣,肖洪彬,牛雯颖,李凤金.丹参注射液对高脂血症模型小鼠脂质代谢的影响[J].湖南中医药大学学报,2022,42(6):911-916[点击复制] |
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| 丹参注射液对高脂血症模型小鼠脂质代谢的影响 |
| 王语晴,郭婉琴,刘欣欣,肖洪彬,牛雯颖,李凤金 |
| (黑龙江中医药大学, 黑龙江 哈尔滨 150040;黑龙江省中医药科学院, 黑龙江 哈尔滨 150040) |
| 摘要: |
| 目的 明确丹参注射液对高脂乳剂诱导的高脂血症模型小鼠脂质代谢的影响。方法 高脂乳剂灌胃法建立高脂血症小鼠模型，造模成功后随机分为模型组、非特组（非诺贝特26 mg/kg）、丹高组（高剂量丹参注射液5 mL/kg）、丹低组（低剂量丹参注射液2.5 mL/kg），每组10只，另取10只小鼠作为空白组。模型组及各给药组予高脂乳剂继续灌胃，同时各给药组予相应药物，模型组和空白组予生理盐水，各组均连续干预21 d。检测血清甘油三酯（triglyceride,TG）、胆固醇（cholesterol,CHO）、高密度脂蛋白胆固醇（high density lipoprotein-cholesterol,HDL-C）、低密度脂蛋白胆固醇（low density lipoprotein-cholesterol,LDL-C）含量；分离肝脏，称重，计算肝脏系数；检测肝组织中超氧化物歧化酶（superoxide dismutase,SOD）、谷胱甘肽过氧化物酶（glutathione peroxidase,GSH-Px）、丙二醛（malondialdehyde,MDA）活性变化；ELISA法检测血清中3-羟基3-甲基戊二酸单酰辅酶A（3-hydro2xy-3-methylglutaryl coenzyme A,HMG-CoA）、卵磷脂胆固醇酰基转移酶（lecithin cholesterol acyltransferase,LCAT）、脂蛋白脂肪酶（lipoprtein lipase,LPL）及肝组织中肝脂酶（hepatic lipase,HL）、胆固醇7α-羟化酶（cholesterol 7α-hydroxylase,CYP7A1）含量。结果 与空白组比较，模型组TG、CHO、LDL-C、MDA、HMG-CoA含量均明显升高（P＜0.05，P＜0.01），LCAT、LPL、SOD、GSH-Px、HL、CYP7A1含量均明显降低（P＜0.05，P＜0.01），肝脏湿质量、肝脏系数均明显增大（P＜0.01）。与模型组比较，各给药组CHO、LDL-C含量均明显降低（P＜0.05，P＜0.01），LCAT、CYP7A1含量均明显升高（P＜0.05）；非特组TG含量明显降低（P＜0.05），LCAT、CYP7A1含量均明显升高（P＜0.05）；非特组及丹低组SOD、GSH-Px含量均明显升高（P＜0.05），MDA含量均明显降低（P＜0.05）；丹高组HMG-CoA含量明显降低（P＜0.05）。结论 丹参注射液能改善高脂乳剂诱导的小鼠血脂代谢紊乱，减轻氧化应激反应及脂质过氧化损伤，对胆固醇代谢的相关酶表达产生影响，以达到降低血脂的目的。 |
| 关键词: 高脂血症 脂质代谢 丹参注射液 非诺贝特 脂质过氧化 |
| DOI：10.3969/j.issn.1674－070X.２０22.06.007 |
| 投稿时间：2022-04-10 |
| 基金项目:国家自然科学基金项目(82074326);黑龙江省自然科学基金资助项目(LH2019H093)。 |
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| Effect of Danshen Injection on lipid metabolism in hyperlipidemia model mice |
| WANG Yuqing,GUO Wanqin,LIU Xinxin,XIAO Hongbin,NIU Wenying,LI Fengjin |
| (Heilongjiang University of Chinese Medicine, Haerbin, Heilongjiang 150040, China;Heilongjiang Academy of Traditional Chinese Medicine, Haerbin, Heilongjiang 150040, China) |
| Abstract: |
| Objective To clarify the effect of Danshen Injection on lipid metabolism in hyperlipidemia model mice induced by hyperlipidemia emulsion. Methods The hyperlipidemia mouse model was established by intragastric administration of high-fat emulsion, and the successful mice were randomly divided into model group, fenofibrate group (fenofibrate 26 mg/kg), Danshen Injection high-dose group (5 mL/kg) and Danshen Injection low-dose group (2.5 mL/kg). There were 10 mice in each group, and another 10 mice were selected as blank group. The model group and each administration group were given high-fat emulsion for continuous intragastric administration, while each administration group was given corresponding drugs, and the model group and the blank group were given normal saline. Each group was continuously intervened for 21 days. The content levels of serum triglyceride (TG), cholesterol (CHO), high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C) were detected. The liver was separated, weighed and the liver coefficient was calculated. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) in liver were detected. ELISA was used to detect 3-hydro2xy-3-methylglutaryl coenzyme A (HMG-CoA), lecithin cholesterol acyltransferase (LCAT), lipoprtein lipase (LPL) in serum and hepatic lipase (HL), cholesterol 7α-hydroxylase (CYP7A1) in liver. Results Compared with the blank group, the content of TG, CHO, LDL-C, MDA and HMG-CoA in the model group increased significantly (P<0.05, P<0.01), the content of LCAT, LPL, SOD, GSH-Px, HL and CYP7A1 decreased significantly (P<0.05, P<0.01). Compared with the model group, the content of CHO and LDL-C in each treatment group decreased significantly (P<0.05, P<0.01), and the content of LCAT and CYP7A1 increased significantly (P<0.05); the content of TG in fenofibrate group decreased significantly (P<0.05), the content of LCAT and CYP7A1 increased significantly (P<0.05); the content of SOD and GSH-Px in fenofibrate group and Danshen Injection high-dose group increased significantly (P<0.05), and MDA decreased significantly (P<0.05); the content of HMG-CoA in Danshen Injection high-dose group decrease significantly (P<0.05). Conclusion Danshen Injection can improve the disorder of blood lipid metabolism in mice induced by high-fat emulsion, reduce oxidative stress and lipid peroxidation injury, and affect enzymes related to cholesterol metabolism, so as to reduce blood lipid. |
| Key words: hyperlipidemia lipid metabolism Danshen Injection fenofibrate lipid peroxidation |
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