| 引用本文: |
周志华,李耀伟,王志琪,李跃辉,何苗,丛梦静.槲皮素配伍芹菜素对顺铂诱导人胃上皮细胞损伤作用的研究[J].湖南中医药大学学报,2022,42(5):772-778[点击复制] |
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| 槲皮素配伍芹菜素对顺铂诱导人胃上皮细胞损伤作用的研究 |
| 周志华,李耀伟,王志琪,李跃辉,何苗,丛梦静 |
| (湖南中医药大学药学院, 湖南 长沙 410208;湖南省中药饮片标准化及功能工程技术研究中心, 湖南 长沙 410208;湖南省中医药研究院, 湖南 长沙 410006) |
| 摘要: |
| 目的 研究槲皮素配伍芹菜素抗顺铂诱导人胃上皮细胞（GES-1）损伤作用。方法 运用分子对接技术将槲皮素和芹菜素与肿瘤坏死因子-α（tumour necrosis factor-α, TNF-α）、白介素-1β（interleukin-1β, IL-1β）蛋白对接。采用细胞实验方法，将GES-1细胞随机分为空白组、顺铂组、槲皮素+芹菜素组（40 μmol/L槲皮素配伍4 μmol/L芹菜素干预顺铂诱导GES-1细胞损伤）。噻唑蓝（methyl thiazolyl tetrazolium, MTT）法测定细胞存活率；Hoechst 33258染色剂测定细胞凋亡染色；线粒体膜电位探针（JC-1）测定细胞线粒体膜电位；活性氧探针（DCFH-DA）测定细胞内活性氧（reactive oxygen species, ROS）含量；微板法测定谷胱甘肽（glutathione, GSH）含量；硫微量操作法测定丙二醛（malondialdehyde, MDA）含量；钼酸铵法测定过氧化氢酶（catalase, CAT）含量；WST-1法测定超氧化物歧化酶（superoxide dismutase, SOD）含量；ELISA法测定TNF-α、IL-1β表达。结果 与空白组比较，顺铂组能升高GES-1细胞凋亡荧光度值、ROS和MDA含量、TNF-α和IL-1表达，降低线粒体膜电位、GSH、CAT、SOD含量（P<0.05或P<0.01）；与顺铂组比较，槲皮素+芹菜素组能降低顺铂诱导的GES-1细胞凋亡荧光度值、ROS和MDA含量、TNF-α和IL-1表达，升高线粒体膜电位、CAT、GSH、SOD含量（P<0.05或P<0.01）。结论 顺铂能诱导GES-1细胞损伤，槲皮素配伍芹菜素能拮抗顺铂诱导GES-1细胞损伤，其作用与抑制GES-1细胞氧化应激、炎症反应和凋亡有关。 |
| 关键词: 槲皮素 芹菜素 顺铂 GES-1细胞 凋亡 氧化应激 炎症反应 |
| DOI：10.3969/j.issn.1674-070X.2022.05.014 |
| 投稿时间：2021-12-27 |
| 基金项目:国家自然科学基金面上项目（81473617）；国家自然科学基金项目（81503492）；湖南省自然基金科药联合项目（2020JJ9013）；湖南省中医药管理局重点项目（201921）；国家级大学生创新项目（201910541019）。 |
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| Effect of quercetin and apigenin on cisplatin-induced injury of human gastric epithelial cells |
| ZHOU Zhihua,LI Yaowei,WANG Zhiqi,LI Yuehui,HE Miao,CONG Mengjing |
| (School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Research Center of Standardization and Functional Engineering of Traditional Chinese Medicine in Hunan Province, Changsha, Hunan 410208, China;Hunan Academy of Chinese Medicine, Changsha, Hunan 410006, China) |
| Abstract: |
| Objective To study the effect of quercetin and apigenin on human gastric epithelial (GES-1) cell injury induced by cisplatin. Methods Molecular docking technology was used to dock the quercetin and apigenin with tumour necrosis factor-α (TNF-α), interleukin-1β (IL-1β) proteins. Cell experiment method was used, GES-1 cells were randomly divided into blank group, cisplatin group and quercetin+apigenin group (40 μmol/L quercetin and 4 μmol/L apigenin interfered with cisplatin-induced GES-1 cell damage). Cell viability was determined by methyl thiazolyl tetrazolium (MTT) method; Hoechst 33258 was used to determine cell apoptosis staining; 5,5'6,6'-tetrachloro-1,1',3,3'-tetraethyl-imidacarbocyanine iodide (JC-1) was used to detect intracellular mitochondrial membrane potential; reactive oxygen species (ROS) were determined with 2,7-dichlorodidi-hydrofluorescein diacetate (DCFH-DA); glutathione (GSH) content was measured by microplate method; malondialone (MDA) content was measured by sulfur trace manipulation method; catalase (CAT) content was measured by ammonium molybdate method; WST-1 method was used to determine superoxide dismutase (SOD) content; the expression levels of TNF-α and IL-1β were determined by ELISA. Results Compared with blank group, cisplatin group increased fluorescence value of GES-1 cell apoptosis, ROS and MDA content, TNF-α and IL-1β expression, and decreased mitochondrial membrane potential, GSH, CAT and SOD content (P<0.05 or P<0.01); compared with the cisplatin group, quercetin+apigenin group decreased cisplatin-induced fluorescence value of GES-1 cell apoptosis, ROS and MDA content, TNF-α and IL-1β expression, and increased mitochondrial membrane potential, CAT, GSH and SOD content (P<0.05 or P<0.01). Conclusion Cisplatin can induce GES-1 cell injury, quercetin combined with apigenin can antagonize cisplatin-induced cell injury, which may be related to inhibiting cell oxidative stress, inflammatory response and apoptosis. |
| Key words: quercetin apigenin cisplatin GES-1 cells apoptosis oxidative stress inflammatory response |
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