| 引用本文: |
马琳莎,吴玉冰,范晓川,李菁,王焱炜,黄晓峰.枸杞多糖对脂多糖刺激下牙周膜干细胞氧化应激及炎症因子表达影响的初探[J].湖南中医药大学学报,2022,42(5):767-771[点击复制] |
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| 枸杞多糖对脂多糖刺激下牙周膜干细胞氧化应激及炎症因子表达影响的初探 |
| 马琳莎,吴玉冰,范晓川,李菁,王焱炜,黄晓峰 |
| (首都医科大学附属北京友谊医院口腔科, 北京 100050;湖南中医药大学发展规划与医院管理处, 湖南 长沙 410208) |
| 摘要: |
| 目的 探索枸杞多糖（Lycium barbarum polysaccharide）改善牙龈卟啉单胞菌来源脂多糖（lipopolysaccharide, LPS）刺激下牙周膜干细胞的氧化应激水平及炎症因子的表达情况。方法 体外培养人牙周膜干细胞，分为对照组（不加LPS刺激，不加枸杞多糖处理）、炎症组（10 μg /mL LPS）、实验组（分为炎症+50 μg/mL枸杞多糖组、炎症+100 μg/mL枸杞多糖组、炎症+200 μg/mL枸杞多糖组3个亚组，均采用10 μg/mL LPS刺激，之后分别添加50、100、200 μg/mL枸杞多糖处理）。CCK-8检测细胞活性，荧光探针检测活性氧水平，RT-PCR及Western blot检测炎症因子表达。结果 牙周膜干细胞在10 μg/mL LPS刺激下，增殖活力下降，活性氧水平及炎症因子表达上调；枸杞多糖能够提升LPS刺激下的牙周膜干细胞增殖活性，且100 μg/mL与200 μg/mL（P<0.01）枸杞多糖较50 μg/mL（P<0.05）枸杞多糖提升更明显；50 μg/mL枸杞多糖能下调LPS刺激下IL-1β基因表达（P<0.05）；100 μg/mL与200 μg/mL枸杞多糖能下调LPS刺激下活性氧水平（P<0.05），以及TNF、IL-1β基因（P<0.01）和蛋白表达水平（P<0.05）。结论 枸杞多糖能够改善LPS刺激下牙周膜干细胞的活性，减轻活性氧水平及炎症因子表达，保护牙周膜干细胞抗氧化潜能。 |
| 关键词: 枸杞多糖 脂多糖 牙周膜干细胞 氧化应激 炎症因子 |
| DOI：10.3969/j.issn.1674-070X.2022.05.013 |
| 投稿时间：2021-12-07 |
| 基金项目:首都医科大学附属北京友谊医院人才项目“种子计划”（YYZZ201938）。 |
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| Effect of Lycium barbarum polysaccharide on oxidative stress and inflammatory factors expression of periodontal ligament stem cells under lipopolysaccharide stimulation |
| MA Linsha,WU Yubing,FAN Xiaochuan,LI Jing,WANG Yanwei,HUANG Xiaofeng |
| (Department of Stomatology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China;Division of Development Planning and Hospital Management, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To explore the influence of Lycium barbarum polysaccharide (LBP) on the oxidative stress and inflammatory factors expression of periodontal ligament stem cells stimulated by lipopolysaccharide (LPS) derived from porphyromonas gingivalis. Methods Periodontal ligament stem cells were obtained and cultured in vitro, and divided into control group (no LPS stimulation, no LBP treatment), inflammatory group (10 μg/mL LPS) and experimental group (divided into three subgroups of inflammation+50 μg/mL LBP group, inflammation+100 μg/mL LBP group, inflammation+200 μg/mL LBP group, all were treated with 10 μ g/mL LPS stimulation, followed by 50, 100, 200 μg/mL LBP treatment). CCK-8 was conducted to evaluate cell viability. The fluorescent probe was used to detect reactive oxygen species. The inflammatory factors expression was measured by RT-PCR and Western blot. Results The viability of periodontal ligament stem cells was decreased and the expression of reactive oxygen species and inflammatory factors increased under 10 μg/mL LPS stimulation; LBP could enhance the proliferation activity of LPS-stimulated periodontal ligament stem cells, and 100 μg/mL and 200 μg/mL (P<0.01) LBP increased more obviously than 50 μg/mL (P<0.05) LBP; 50 μg/mL LBP could down-regulate IL-1β gene expression under LPS stimulation (P<0.05); 100 μg/mL and 200 μg/mL LBP could down-regulate the level oreactive oxygen species (P<0.05), TNF, IL-1β gene (P<0.01) and protein expression (P<0.05) under LPS stimulation. Conclusion LBP can improve the viability of LPS-stimulated periodontal ligament stem cells and alleviated the reactive oxygen species level and inflammatory factors expression, and protect the antioxidant potential of periodontal ligament stem cells. |
| Key words: Lycium barbarum polysaccharide lipopolysaccharide periodontal ligament stem cell oxidative stress inflammatory factors |
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